ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF LAPORTEA INTERRUPTA LINN (original) (raw)
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Antioxidant Activity of Laportea decumana (Roxb) Wedd Ethanol and n-Hexane Extracts
Open Access Macedonian Journal of Medical Sciences, 2022
BACKGROUND: Laportea decumana (Robx) wedd was a traditional medicine from Indonesian, especially in Eastern Indonesia, Ambon-Maluku. Based on a literature search, it was found that Laportea decumana (Robx) wedd has very little discussion, and most of its research comes from Indonesia, which is most likely endemic in Indonesia. This makes it interesting to develop it into herbal medicine whose use is based on evidence-based research so that it is safe when used as complementary nursing therapy. AIM: This study tried to identify the phytochemical composition and investigate the antioxidant activity of Laportea decumana (Robx) wedd extract from Ambon-Maluku. METHODS: Extraction is done by the maceration method with 96% ethanol solvent. Phytochemical tests are performed with thin-layer chromatography (TLC) to look at flavonoids, tannins, triterpenoids, then alkaloids, and saponins using tube tests. This study tested antioxidant activity using the DPPH free radical scavenging method. RESULTS: Laportea decumana (Robx) wedd phytochemical test positively contains alkaloids, flavonoids, tannins, triterpenoids, and saponins. Laportea decumana (Robx) wedd phytochemical test positively contains alkaloids, flavonoids, tannins, triterpenoids, and saponins. Laportea decumana (Robx) Wedd has a powerfull antioxidant ability which is obtained Laportea decumana (Robx) wedd ethanol extract (LDrW-EE) 22.81 μg/ml, Laportea decumana (Robx) wedd n-hexane extract (LDrW-NHE) 44.89 μg/ml and vitamin C 6.03 μg/ml. CONCLUSION: The antioxidant activity of LDrW-EE is better than LDrW-NHE but not better than Vitamin C. Laportea decumana (Roxb) wedd extract can be developed as a complementary nursing therapy because it is included in the category of strong antioxidants.
Human life is depending on plants for various needs including for food and medicine. The indigenous medicine system of every country is using plant and plant products as their basic material for preparation of medicine. Laportea interrupta L. Chew commonly known as 'stinging nettle' is one of the ethnomedicinal plants of India claimed for various medicinal uses like anti-inflammatory, anti-pyretic and antimicrobial agent. However, the plant has not been much explored for its chemical characteristics. The present study has carried out detailed Physico-chemical, Phytochemical and Spectroscopic Characteristics of aqueous and methanolic extracts of Laportea interrupta L. Leaf. Phytochemical study showed the presence of flavonoids, glycosides, saponins and alkaloids. The spectroscopic studies showed the presence of various group of compounds flavonoids, xanthophylls, phycobilins in methanol and aqueous extract predominantly. These study parameters will be highly helpful to take up the new studies on various aspects of its biological activity.
Human life is depending on plants for various needs including for food and medicine. The indigenous medicine system of every country is using plant and plant products as their basic material for preparation of medicine. Laportea interrupta L. Chew commonly known as 'stinging nettle' is one of the ethnomedicinal plants of India claimed for various medicinal uses like anti-inflammatory, anti-pyretic and antimicrobial agent. However, the plant has not been much explored for its chemical characteristics. The present study has carried out detailed Physico-chemical, Phytochemical and Spectroscopic Characteristics of aqueous and methanolic extracts of Laportea interrupta L. Leaf. Phytochemical study showed the presence of flavonoids, glycosides, saponins and alkaloids. The spectroscopic studies showed the presence of various group of compounds flavonoids, xanthophylls, phycobilins in methanol and aqueous extract predominantly. These study parameters will be highly helpful to take up the new studies on various aspects of its biological activity.
2019
Laportea aestuans (Gaud) belonging to the family Urticaceae is an herbaceous weed consumed as vegetable and used in traditional medicine. In continuation of our work on this plant, bioactive constituents were isolated using chromatographic technique and the structures of all isolated constituents were determined by spectroscopic techniques and comparison with literature data. Enzyme inhibition (urease and α-glucosidase inhibition activities), free radical scavenging and insecticidal activities of the crude extracts and isolates were also determined. Isolation and characterization afforded six known constituents among which are 5, 2', 4' trihydroxy 7, 8 dimethoxy flavones (LA4) (29.8± 0.15) and 15-hydroxycryptopleurine (LA6) (18.5± 0.54) that showed significant urease inhibition activity, 4-methoxy-8'-acetyl olivil (LA1) (14.9± 0.43) and 5, 2', 4' trihydroxy 7, 8 dimethoxy flavones (LA4) (14.6± 0.12) which had significant α-glucosidase inhibition activity. 5, 2', 4' trihydroxy 7, 8 dimethoxy flavones (LA4) (15.45±0.01), 3-(4-hydroxyphenyl)-4-(3-methoxy-4-hydroxyphenyl)-3,4dehydroquinolizidine (LA5) (20.27±0.00) and 15-hydroxycryptopleurine (LA6) (28.81±0.03) were the most active in the free radical scavenging assay when activities were compared with thiourea, 1-deoxynojiromycin hydrochloride (DNJ) and butylated hydroxylanisole used as standards in the three assays. This study therefore added more information to the ethnomedicinal importance of L. aestuans used in traditional medicine.
2017
To evaluate thrombolytic, membrane stabilizing & cytotoxic activities of Laportea interrupta Linn. (Family-Urticaceae) the whole plants were extracted with ethanol and were partitioned with petroleum ether, carbon tetrachloride and water for biological investigation. Different partitionates of L. interrapta protected the haemolysis of RBC induced by hypotonic solution and heat as compared to standard Acetyl salicylic acid. In our study ethanol extract of L. interrupta showed highest protection of 81 ± 0.095 by hypnotic solution and in heat 50 ± 0.496 respectively. The pet. ether soluble fraction of L. interrupta displayed highest thrombolytic activity (48 ± 0.207)% where Streptokinase was used as a positive control to the clots. In the brine shrimp lethality bioassay, pet. ether soluble fraction (PESF) showed significant lethality having the LC50 value 7.36 μg/ml. The obtained data provide a support for the use of our plant in traditional medicine and its further investigation. Keyw...
To evaluate thrombolytic, membrane stabilizing & cytotoxic activities of Laportea interrupta Linn. (Family-Urticaceae) the whole plants were extracted with ethanol and were partitioned with petroleum ether, carbon tetrachloride and water for biological investigation. Different partitionates of L. interrapta protected the haemolysis of RBC induced by hypotonic solution and heat as compared to standard Acetyl salicylic acid. In our study ethanol extract of L. interrupta showed highest protection of 81 ± 0.095 by hypnotic solution and in heat 50 ± 0.496 respectively. The pet. ether soluble fraction of L. interrupta displayed highest thrombolytic activity (48 ± 0.207)% where Streptokinase was used as a positive control to the clots. In the brine shrimp lethality bioassay, pet. ether soluble fraction (PESF) showed significant lethality having the LC50 value 7.36 μg/ml. The obtained data provide a support for the use of our plant in traditional medicine and its further investigation.
Journal of Medicinal Plants Studies, 2017
The study evaluated antioxidant potentials of Laportea aestuans and chemopreventive potentials on urinary inflammatory markers using albino rats. The rats were fed with 10%, 30% and 50% Laportea aestuans. Results indicates a reduction in nitric oxide levels compared to control (P<0.05) while concentration of polyphenols in urine were significant with increased percentage of Laportea aestuans supplemented diet. This study also showed reductive potencies of Laportea aestuans in the concentration of protein in urine. Results also showed an increase in the activities of CyP1A1 microsomal enzymes in the test groups compared to control (P<0.05). The activities of Glutathion Stransferase, Quinone oxidoreductase and Thioredoxin reductase increased significantly in all the test samples with increasing percentage of Laportea aestuans compared to control. This is indicative of the potential health benefit of Laportea aestuans in chemoprevention. On this basis, Laportea aestuans have show...
2010 Khura et al Antioxidant and Antimicrobial Activity
Centella asiatica, traditionally used in ethno medicines to treat common infections and various disorders, have been studied for their antimicrobial and antioxidant activity. The methanolic extracts of the plant leaves exhibited significant and dose-dependent antioxidant activities in DPPH radical scavenging, ferric ion reducing and phosphomolybdate assays. These leaf extracts showed antimicrobial activity against selected Gram +ve and Gram -ve bacterial strains. A. vasica and L. indica extracts possessed maximum antioxidant and antimicrobial activity, respectively. The activities could be correlated to phenolics and flavonoid content of the leaf extracts which ranged from 30.25 to 91.98 mg GAE g -1 dw leaf extract and 2.67 to 96.45 mg RE g -1 dw leaf extract respectively. The aqueous extracts of plant leaves significantly protected the DNA damage against the oxidative damage caused by hydroxyl radicals.
BMC Chemistry
Background: As possible sources of natural bioactive molecules, the plant essential oils and extracts have been used globally in new antimicrobial compounds, food preservatives, and alternatives to treat infectious disease. Methods: In this research, the antimicrobial activities of chloroformic and methanolic extracts of Sophora flavescens, Rhaponticum repens, Alhagi maurorum, Melia azedarach, Peganum harmala, and Juncus conglomeratus were evaluated against 8 bacteria (S. aureus, B. subtilis, R. toxicus, P. aeruginosa, E. coli, P. syringae, X. campestris, P. viridiflava) and 3 fungi (Pyricularia oryzae, Fusarium oxysporum and Botrytis cinerea), through disc diffusion method. Furthermore, the essential oils of plants with the highest antibacterial activity were analyzed utilizing GC/MS. Moreover, the tested plants were exposed to screening for possible antioxidant effect utilizing DPPH test, guaiacol peroxidas, and catalase enzymes. Besides, the amount of total phenol and flavonoid of these plants was measured. Results: Among the tested plants, methanolic and chloroformic extracts of P. harmala fruits showed the highest antibacterial activity against the tested bacteria. Besides, the investigation of free radical scavenging effects of the tested plants indicated the highest DPPH, protein, guaiacol peroxidase, and catalase in P. harmala, M. azedarach, J. conglomeratus fruits, and J. conglomeratus fruits, respectively. In addition, the phytochemical analysis demonstrated the greatest amounts of total phenolic and flavonoid compositions in J. conglomeratus and P. harmala, respectively. Conclusion: The results indicated that these plants could act as a promising antimicrobial agent, due to their short killing time.
Antioxidant and Free Radical Scavenging Activity of Certain Dietary and Medicinal Plant Extracts
The antioxidant and free radical scavenging activity of the ethanol extracts of Melia dubia Hiern., Albizia lebbeck (L.) Benth., Brassica oleracea L. var. botrytis (broccoli) and Glycyrrhiza glabra L. were evaluated. The total phenolic content of the extracts was found to be higher in A. lebbeck (33.6%) and lower in broccoli (16.9%). The antioxidant activity of the extract was evaluated through different chemical assays and in vitro methods such as reducing power, DPPH x , ABTS x+ and x OH radical scavenging activities, peroxidation inhibition in linoleic acid emulsion system and antihemolytic activity. The increasing concentrations of the extract exhibited nonlinear increases in the reducing power. Extract of A. lebbeck was found to have more hydrogen donating ability. Similar line of dose dependant activity has been observed in DPPH x and x OH radical scavenging systems. Further, A. lebbeck extracts exhibited strong antioxidant activity equivalent to trolox (6423.04 μmol g-1) in ABTS radical cation scavenging system. All the extracts exhibited notable activity against linoleic acid emulsion system (35.5 to 40.2%) and antihemolytic activity (29.8 to 46.6%).