Biodegradation and corrosion behavior of manganese oxidizer Bacillus cereus ACE4 in diesel transporting pipeline (original) (raw)
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Corrosion Science 2007 Rajasekar et al
The degradation problem of petroleum products arises since hydrocarbon acts as an excellent food source for a wide variety of microorganisms. Microbial activity leads to unacceptable level of turbidity, corrosion of pipeline and souring of stored product. The present study emphasizes the role of Bacillus cereus ACE4 on degradation of diesel and its influence on corrosion of API 5LX steel. A demonstrating bacterial strain ACE4 was isolated from corrosion products and 16S rRNA gene sequence analysis showed that it has more than 99% similarity with B. cereus. The biodegradation and corrosion studies revealed that B. cereus degraded the aliphatic protons and aromatic protons in diesel and is capable of oxidizing ferrous/manganese into oxides. This is the first report that discloses the involvement of manganese oxidizer B. cereus ACE4 on biodegradation of diesel and its influence on corrosion in a tropical country pipeline.
This paper reports the microbiologically induced corrosion (MIC) and electrochemical behavior of carbon steel (API 5LX) in the presence of hydrocarbon-degrading bacteria Bacillus cereus ACE4 (a Gram-positive bacterium) and Serratia marcescens ACE2 (a Gram-negative bacterium). Weight loss studies and metallographic analysis of the metal API 5LX exposed to a simulated corrosive environment showed that the bacterium ACE4 caused severe pitting corrosion than that of bacterium ACE2. As part of biodegradation studies, the impact of aryl hydrocarbon hydroxylase (AHH) on diesel degradation was investigated along with reduction of total hydrocarbons. It was clearly observed that, during the biodegradation experiment in the presence of B. cereus ACE4, the content of the total hydrocarbons decreased significantly due to their metabolism induced by AHH enzymes when compared to S. marcescens ACE2. Degraded petroleum hydrocarbons (diesel) act as a good nutrient for bacteria, which in turn increases the proliferation of bacteria on the steel and determines the nature of corrosion. Metal oxides such as MnO 2 and Fe 2 O 3 were found as part of the corrosion products, indicating that the ACE4 bacterium is capable of converting the elements on the carbon steel (API 5LX) to their metal oxides and thus accelerating severe pitting corrosion on the surface of the pipeline networks. Overall, the study provides an insight into the microbiologically influenced corrosion of carbon steel API 5LX by two hydrocarbon-degrading bacteria in diesel fuel/water mixtures.
Biodegradation of corrosion inhibitors and their influence on petroleum product pipeline
Microbiological Research, 2007
The present study enlightens the role of Bacillus cereus ACE4 on biodegradation of commercial corrosion inhibitors (CCI) and the corrosion process on API 5LX steel. Bacillus cereus ACE4, a dominant facultative aerobic species was identified by 16S rDNA sequence analysis, which was isolated from the corrosion products of refined diesel-transporting pipeline in North West India. The effect of CCI on the growth of bacterium and its corrosion inhibition efficiency were investigated. Corrosion inhibition efficiency was studied by rotating cage test and the nature of biodegradation of corrosion inhibitors was also analyzed. This isolate has the capacity to degrade the aromatic and aliphatic hydrocarbon present in the corrosion inhibitors. The degraded products of corrosion inhibitors and bacterial activity determine the electrochemical behavior of API 5LX steel.
Petroleum contamination and its derivate in ecosystem are considered as environmental threat all over the world. Some microorganisms exhibit potential to degrade hydrocarbon in contaminated environments. This study aims at identifying potential diesel oil-degrading bacteria grown on artificial media. Bacteria isolated from Manado Sea port were grown in nutrient agar containing artificial diesel oil plus salt water and diesel oil only, respectively. The growing bacteria were isolated and each of them was grown separately to obtain pure isolate. Three bacterial isolates namely AO2, OA3 and OA4 were identified using 16S rRNA gene as Pseudomonas aeroginosa, Klebsiella oxytoca, and Citrobacter sp, respectively. Keywords: diesel oil, diesel oil-degrading bacteria, Manado Sea Port, 16S rRNA gene IDENTIFIKASI BAKTERI YANG BERPOTENSI SEBAGAI PENDEGRADASI MINYAK DIESEL DI ISOLASI DARI PELABUHAN LAUT MANADO ABSTRAK Kontaminasi minyak bumi dan turunannya dalam ekosistem dianggap sebagai ancaman...
Isolation and characterization of a novel native Bacillus strain capable of degrading diesel fuel
International Journal of Environmental Science & Technology, 2009
The ability of native bacteria to utilize diesel fuel as the sole carbon and energy source was investigated in this research. Ten bacterial strains were isolated from the oil refinery field in Tehran, Iran. Two biodegradation experiments were performed in low and high (500 and 10000 ppm, respectively) concentration of diesel fuel for 15 days. Only two isolates were able to efficiently degrade the petroleum hydrocarbons in the first test and degraded 86.67 % and, 80.60 % of diesel fuel, respectively. The secondary experiment was performed to investigate the toxicity effect of diesel fuel at high concentration (10000 ppm). Only one strain was capable to degrade 85.20 % of diesel fuel at the same time (15 days). Phenotype and phylogeny analysis of this strain was characterized and identified as dieseldegrading bacteria, based on gram staining, biochemical tests, 16S rRNA gene sequence analysis. These results indicate that this new strain was Bacillus sp. and could be considered as Bacillus Cereus with 98 % 16 S rRNA gene sequence similarity. The results indicate that native strains have great potential for in situ remediation of diesel-contaminated soils in oil refinery sites.
Microbiologically influenced corrosion in oil and gas industries: A review
International Journal of Corrosion and Scale Inhibition, 2021
Microbial Corrosion or more commonly known as Microbiologically Influenced Corrosion (MIC) is of significant interest and importance to the oil and gas industry. Microorganisms such as sulphate reducing bacteria (SRB), sulphate oxidizing bacteria (SOB), Metal oxidizing and reducing bacteria are responsible of MIC, with SRB to be considered as major cause of MIC in pipeline systems. Reservoir souring, leakage in storage tanks, and equipment and pipeline failures are some of the key issues faced by this industry due to MIC. With 75% and 50% shares in the oil well failures and internal pipeline corrosion respectively, it has now become a prime area of research in terms of understanding the microbial species, their interactions with metals and other microorganisms, followed by detection, monitoring and control of MIC. This paper/project reviews the current understanding of MIC in oil and gas industry that has been developed through research activities over the recent past. Microorganisms associated with corrosion are introduced, after which the details of mechanisms involved in an MIC occurrence are presented. Conventional and advanced characterization, monitoring and inspections techniques for MIC are discussed in detail. Various methods for prevention and control are presented such as cleaning methods, use of biocides, coatings, proper material selection, and cathodic protection. Excerpts of case studies are included to provide the insights on how research is being conducted in the industry towards MIC occurrences. It is found that MIC is a function of a host of parameters which are common in oil and gas industries, making them extremely vulnerable to this severe form of corrosion.
The present work was conducted to isolate and identify bacteria from oil- 21 contaminated soil to evaluate their role in biodegradation of commercial diesel under laboratory 22 conditions. Diesel fuels are used by different vehicles, diesel generators and especially heavy 23 transport vehicles. Its manufacturing, transportation, utilization and disposal have the threat to 24 pollute the surrounding environment. Biodegradation is one of the biological processes to 25 remediate the pollutants. This is the cheaper and easy method as compared to other methods like 26 direct burning, land foaming and bioventing because diesel smoke has a carcinogenic effect. 27 In the current exploration ordinary bacterial species that degrade diesel were isolated from 28 selected areas of Mansehra. Six sites were selected and a total of 60 samples were collected. 29 These sites were coded according to their names. For the isolation of diesel degrading bacteria 30 soil samples were analyzed. The isolation of bacteria was done on nutrient agar, nutrient broth 31 and mineral salt medium (MSM), and IR spectroscopy was used to observe the degradation of 32 diesel. Twenty samples were found having bacterial growth. Further analysis showed that four 33 samples BHG, SHNK, TAK and KMOR had diesel degrading capability as these showed 34 positive results. The isolated bacteria were identified by morphological and biochemical features, 35 while the degradation of diesel was determined qualitatively by interpreting the intensity of the 36 peaks of IR spectroscopy. The intensity of the peaks in the spectrum of control was compared 37 with the intensity of the peaks in the spectrum of the test samples which indicated degradation of 38 diesel. Three bacterial species, namely, Bacillus cereus, Bacillus subtilis and Pseudomonas 39 aeruginosa were identified using Berge's manual method. The bacterial species were tested for 40 their capability to degrade commercial diesel presented at different concentrations i.e. 1000 ppm 41 and 5000 ppm.
Research Square (Research Square), 2024
This research focused on investigating a case of an Egyptian petroleum company, where their elds faced Microbiologically in uenced corrosion (MIC) problems, even though they had been using corrosion inhibitors and biocides. Therefore, ve samples were collected and used to study their microbial community using 16S metagenomics sequencing. The result displayed many potential corrosive bacteria that represented in families, genera and species. The samples were studied concerning total bacterial counts, acid-producing bacteria (APB), slime-forming bacteria (SFB), sulfate-reducing bacteria (SRB). The results displayed that; total bacterial counts were Nil in all samples except one. All samples did not grow in speci c media for APB. All the samples displayed positive growth results on SFB speci c media. Moreover, only one sample has demonstrated a positive result on SRB speci c media. One sample was selected to study its corrosion activities upon its diver's bacterial community, cultivable on microbially speci c media and its real-eld corrosion severity. The SFB corrosion s activities of SRB were assessed by examining alterations in dissolved sul de, redox potential and metal corrosion rate. Surface morphology was examined using scanning electron microscopy.