The application of novel mussel-inspired compounds in dentistry (original) (raw)
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The application of mussel-inspired molecule in dentin bonding
Journal of Dentistry, 2020
The application of mussel-inspired molecule in dentin bonding Kang Li (Conceptualization) (Methodology) (Investigation) (Formal analysis) (Writing-original draft), Yuhong Sun (Investigation) (Formal analysis), James Kit Hon Tsoi (Supervision) (Writingreview and editing), Cynthia Kar Yung Yiu (Supervision) (Writingreview and editing) (Funding acquisition)
Biomedical and Clinical Importance of Mussel-Inspired Polymers and Materials
Marine Drugs, 2015
The substance secreted by mussels, also known as nature's glue, is a type of liquid protein that hardens rapidly into a solid water-resistant adhesive material. While in seawater or saline conditions, mussels can adhere to all types of surfaces, sustaining its bonds via mussel adhesive proteins (MAPs), a group of proteins containing 3,4-dihydroxyphenylalanine (DOPA) and catecholic amino acid. Several aspects of this adhesion process have inspired the development of various types of synthetic materials for biomedical applications. Further, there is an urgent need to utilize biologically inspired strategies to develop new biocompatible materials for medical applications. Consequently, many researchers have recently reported bio-inspired techniques and materials that show results similar to or better than those shown by MAPs for a range of medical applications. However, the susceptibility to oxidation of 3,4-dihydroxyphenylalanine poses major challenges with regard to the practical translation of mussel adhesion. In this review, various OPEN ACCESS Mar. Drugs 2015, 13 6793 strategies are discussed to provide an option for DOPA/metal ion chelation and to compensate for the limitations imposed by facile 3,4-dihydroxyphenylalanine autoxidation. We discuss the anti-proliferative, anti-inflammatory, anti-microbial activity, and adhesive behaviors of mussel bio-products and mussel-inspired materials (MIMs) that make them attractive for synthetic adaptation. The development of biologically inspired adhesive interfaces, bioactive mussel products, MIMs, and arising areas of research leading to biomedical applications are considered in this review.
Dental Adhesion Enhancement on Zirconia Inspired by Mussel's Priming Strategy Using Catechol
Zirconia has recently become one of the most popular dental materials in prosthodontics being used in crowns, bridges, and to implants. However, weak bonding strength of dental adhesives and resins to zirconia surface has been a grand challenge in dentistry, thus finding a better adhesion to zirconia is urgently required. Marine sessile organisms such as mussels use a unique priming strategy to produce a strong bonding to wet mineral surfaces; one of the distinctive chemical features in the mussel’s adhesive primer proteins is high catechol contents among others. In this study, we pursued a bioinspired adhesion strategy, using a synthetic catechol primer applied to dental zirconia surfaces to study the effect of catecholic priming to shear bonding strength. Catechol priming provided a statistically significant enhancement (P < 0.05) in shear bonding strength compared to the bonding strength without priming, and relatively stronger bonding than commercially available zirconi...
Pharmaceutics
A synthetic route for adhesive core-multishell (CMS) nanocarriers for application to the oral mucosa was established using mussel-inspired catechol moieties. The three CMS nanocarriers with 8%, 13%, and 20% catechol functionalization were evaluated for loading capacity using Nile red, showing an overall loading of 1 wt%. The ability of Nile red loaded and functionalized nanocarriers to bind to a moist mucosal surface was tested in two complementary adhesion assays under static and dynamic conditions using monolayers of differentiated gingival keratinocytes. Adhesion properties of functionalized nanocarriers were compared to the adhesion of the non-functionalized nanocarrier. In both assays, the CMS nanocarrier functionalized with 8% catechol exhibited the strongest adhesion compared to its catechol-free counterpart and the CMS nanocarriers functionalized with 13% and 20% catechol.
A Simple Strategy to Achieve Mussel-Inspired Highly Effective Antibacterial Coating
Macromolecular Materials and Engineering
effectively prevent the initial attachment of bacteria to implant and device surfaces and subsequently avoid the device-related infection. [18-21] In order to obtain such antibacterial coatings, several mussel-inspired polymers, including polydopamine, [22,23] catechol-derived poly(ethylene glycol), [24,25] and poly(acrylate-co-acrylamide), [26] were chosen as the coating matrixes. Many ingredients, such as Cu 2+ , [27] silver nanoparticle, [28,29] amphiphilic groups, [30] antibiotic, [31,32] peptide, [33] and natural antibacterial agent [34-36] (i.e., tannin, chitosan, and bronel) have been incorporated into these matrix for antibacterial studies. Although these mentioned coatings are effective in prohibition of bacterial growth on surfaces, sophisticated procedures such as surface-initiated polymerization, layerby-layer deposition, and chemical grafting are generally involved in the fabricating process. [37-40] Thus, to develop more facile and simpler methods for preparing mussel-inspired antibacterial coatings are still challenging and deserve further study. Investigations on the attachment plaques from various mussels reveal that the reaction of catechol and amine group (in lysine residues) via the Michael addition or Schiff base reactions play a critical role in the formation of water-insoluble, 3D marine adhesive proteins (MAP) networks. [5,12,41-45] Inspired by this characteristic, Li et al. developed a water-resistant wood adhesive by simply mixing two commercialized materials, branched polyethyleneimine (PEI), a low-cost and amine groups-enriched industrially material and tannin (TA), a natural product rich in catechol groups. [46] Subsequently, this simple and low-cost technology was also used to fabricate functional coatings. [47-50] For example, Wang et al. fabricated hydrophilic modified polypropylene separators by the co-deposition of catechol and polyamine for Li-ion batteries. [51] Xu et al. reported the co-deposition of catechol and PEI onto nanofiltration membrane for textile wastewater treatment. [52] What's more, based on this technology, the usage of noneconomic catechol-containing materials such as dopamine (including polydopamine) or synthetic catechol-derived polymers was also avoided. Nevertheless, to our best knowledge, this simple and facile method has not been applied in the design of mussel-inspired antibacterial coatings. In this study, we, for the first time, synthesized a musselinspired antibacterial coating via the simple co-deposition of PEI and a quaternized catechol (QCat) in an aqueous Antibacterial Coatings Although significant progress has been made in the preparation of musselinspired antibacterial coatings, continual challenges still remain in pursuing more facile and simpler fabrication methods to construct more robust and effective coatings. In this study, quaternized catechol (QCat), which is synthesized via a simple quaternization reaction from two commercially available materials, 2-chloro-3′,4′-dihydroxyacetophenone and N,N-dimethyldodecylamine, is used as a reactive antimicrobial agent to fabricate musselinspired antibacterial coatings. Specifically, QCat reacts with branched polyethyleneimine (PEI) in Tris-HCl solution through a cross-linking reaction between amino and catechol groups to form a homogeneous coating on various substrates via a simple co-deposition process. The formed PEI/QCat coating exhibits highly effective antimicrobial activity against both Staphylococcus aureus and Escherichia coli and good adhesion on glass, metal, and plastic substrates. Such a simple fabrication process makes it a potential candidate for industrial and medical applications.
High-performance mussel-inspired adhesives of reduced complexity
Despite the recent progress in and demand for wet adhesives, practical underwater adhesion remains limited or non-existent for diverse applications. Translation of mussel-inspired wet adhesion typically entails catechol functionalization of polymers and/or polyelectrolytes, and solution processing of many complex components and steps that require optimization and stabilization. Here we reduced the complexity of a wet adhesive primer to synthetic low-molecular-weight catecholic zwitterionic surfactants that show very strong adhesion (B50 mJ m À 2) and retain the ability to coacervate. This catecholic zwitterion adheres to diverse surfaces and self-assembles into a molecularly smooth, thin (o4 nm) and strong glue layer. The catecholic zwitterion holds particular promise as an adhesive for nanofabrication. This study significantly simplifies bio-inspired themes for wet adhesion by combining catechol with hydrophobic and electrostatic functional groups in a small molecule.
Interfacial pH during mussel adhesive plaque formation
Biofouling, 2015
Mussel (Mytilus californianus) adhesion to marine surfaces involves an intricate and adaptive synergy of molecules and spatio-temporal processes. Although the molecules, such as mussel foot proteins (mfps), are well characterized, deposition details remain vague and speculative. Developing methods for the precise surveillance of conditions that apply during mfp deposition would aid both in understanding mussel adhesion and translating this adhesion into useful technologies. To probe the interfacial pH at which mussels buffer the local environment during mfp deposition, a lipid bilayer with tethered pH-sensitive fluorochromes was assembled on mica. The interfacial pH during foot contact with modified mica ranged from 2.2 to 3.3, which is well below the seawater pH of ~ 8. The acidic pH serves multiple functions: it limits mfp-Dopa oxidation, thereby enabling the catecholic functionalities to adsorb to surface oxides by H-bonding and metal ion coordination, and provides a solubility s...
Marine mussel adhesion: biochemistry, mechanisms, and biomimetics
Journal of Adhesion Science and Technology, 2012
Common blue mussel (Mytilus edulis) is a sessile organism that has unique ability to attach to a wide array of organic and inorganic marine surfaces using its holdfast structures. Strong adhesion to surfaces is essential for mussel survival, movement, and self-defense. Mussel proteins from byssal thread are structural components connecting soft mussel tissues to marine surfaces via an adhesive plaque in the distal end, while adhesive proteins from byssal plaque are responsible for mussel adhesion. Adhesive proteins are small molecules containing a high proportion of post-translationally modified amino acids such as 3,4-dihydroxyphenylalanine (DOPA). High DOPA content, small molecular size, protein flexibility, the presence of metal ions, and a high oxidation state enable strong mussel adhesion to surfaces. Mussel adhesion mechanisms depend on the composition and interactions of mussel proteins, as well as their interactions with the environment. Difficulties in the extraction of mussel adhesion proteins hamper mechanism studies and their practical applications. Development of recombinant mussel proteins and biomimetics will advance our understanding of adhesion mechanisms. In this paper, recent advances in the characterization of mussel adhesive proteins (MAPs), mussel adhesion mechanisms, application of MAPs, and the development of biomimetic biopolymers are reviewed.