Example of the application the microsatellite DNA fragments in the study of farmed European catfish (Silurus glanis, L.) broodstock (original) (raw)
Related papers
Marine Biotechnology, 2003
Sea trout (Salmo trutta) is an anadromous form of brown trout, a commercially important salmonid species in Europe. Stocking has been used to compensate for the decrease of natural populations and maintenance of fishery and angling catches. Over 1.5 million smolts and 4.5 million alevins are released to Polish coastal rivers each year. Variation at 7 microsatellite loci (Ssa197, Ssa171, Ssa85, Str15, Str73, Str591, and Str543) was used to study genetic polymorphism of spawners returning to 6 rivers. Application of distance method for comparison of pairs of populations based on number of different alleles (F ST ) revealed significant differences between Vistula and Wieprza, and Parseta as well as between Drweca and Wieprza, and Slupia. The level of heterozygosity was similar between most of the studied sea trout populations; considerable differences were found only for Str591. Differences in frequencies of a few alleles between populations were observed. An exact test of sample differentiation based on allele frequencies confirmed lack of significance of differentiation between the 6 pairs of populations (F ST and R ST ). No admixture was observed in the studied populations.
Archives of Polish Fisheries, 2000
The genetic variability of two farmed strains of brook trout, Salvelinus fontinalis (Mitchill), was examined using six microsatellite DNA loci. The objective of the present study was to evaluate locus-specific genetic markers in brook trout strains cultured in Poland and to assess their levels of genetic diversity. The average number of alleles at all loci in the studied stocks differed between the two groups: 2.20 in the Rutki strain and 7.20 in the Canada strain. For the microsatellites examined, the number of alleles per locus ranged from 1 (locus Ssa-171 in the Canada strain) to 24 (locus Sfo-292 in the Canada strain). The results suggested that the Canada strain had higher gene diversity than the Rutki strain. The molecular analysis described in the present study will be useful for monitoring long-term genetic variation and for the identification of suitable parents for the development of stocks with suitable gene diversity in cultured Polish brook trout.
Indonesian Aquaculture Journal
Genetic diversity is an important aspect of a selective breeding program to produce fish broodstock carrying superior traits such as fast-growing, disease resistant, and other traits. We have carried out a breeding program to produce a fast-growing striped catfish (Pangasianodon hypophthalmus) since 2010. The aim of this study was to evaluate the genetic variation of the first (G-1) and second (G-2) generations of fast-growing striped catfish using microsatellite analysis. The G-1 and G-2 populations were selected individually from populations. DNA samples were collected from 40 ind. fish of each population and analyzed using five microsatellite loci (Pg1, Pg2, Pg3, Pg13, and Pg14). The results showed that the number of alleles per loci in the G-1 and G2 populations ranged from 4 to 7 alleles, with an average of five for each generation. The average of observed heterozygosity of the G-1 population (0.420) was lower than the G-2 population (0.495). Inbreeding level showed that the G-...
Sea trout, Salmo trutta L., populations in the Slupia River basin have been affected by mass stocking with smolts and fry. This work is focused on a small-scale differentiation in sea trout populations from one basin with a strong emphasis on the relationship between wild and hatchery origin fish. A total of 216 sea trout were genotyped at 10 microsatellite loci. Global F ST obtained by AMOVA was low at 0.0165. Pairwise F ST were significant for all tests except wild and stocked adults. The highest pairwise difference was found between the hatchery sample and Kwacza (F ST = 0.038). Analysis of the genetic structure revealed micro-geographical differentiation with four subpopulations. The quality of the artificial spawning was found not to be adequate with a high risk of adverse effects to the whole population. All future stocking actions in the basin should consider the existing population structures.
Polish Journal of Natural Science, 2010
Six microsatellite loci were used to examine the genetic variability of Russian sturgeon and sterlet cultured in a Polish fish farm. Within 47 individuals of Russian sturgeon and 35 individuals of sterlet, a total of 113 alleles were detected in six polymorphic microsatellite loci. The number of alleles per locus ranged from 9 to 23 in Russian sturgeon and 3-8 in sterlet. The genetic diversity of six microsatellite loci varied from 0.404 to 0.880 in Russian sturgeon and from 0.515 to 0.971 in sterlet. Microsatellite analysis has a great potential for aquaculture of sturgeon fishes, especially when levels of genetic variation could be monitored and inbreeding controlled in commercial breeding programs.
Acta Biologica, 2017
Common whitefish (Coregonus maraena) in Poland belongs to the endangered species. The degradation of the environment causes common whitefish to lose its natural reproduction sites. The natural genetic structure of whitefish has been compromised by anthropogenetic activities involving eutrophication, river regulation, the introduction of non-native species and as well as excessive exploitation of the species. The genetic variability of common whitefish (Coregonus maraena) from 2 sites: Pomeranian Bay and the lower Oder river, was assessed using microsatellite markers. A total of 45 caught individuals were analysed (26 from Pomeranian Bay and 19 from the Oder river). Polymorphism at nine loci, Str60INRA,
Genetic diversity of Cyprinus carpio from an Albanian fish hatchery based on microsatellite markers
2019
The study is focused on the genetic characterization of Cyprinus carpio from a fish hatchery in used for the population of ponds of the semi-intensive fish farms in central part of Albania. A total of 30 specimens from this fish hatchery were genotyped for four microsatellite markers. All markers were highly polymorphic, with the number of alleles higher than 14 and PIC values higher than 0.5. Observed and expected heterozygosity values were found as 0.609 and 0.887, respectively, and the mean fixation index was calculated as 0.312. All loci showed significant deviation from Hardy Weinberg equilibrium. Genetic bottleneck analysis did not reveal any recent bottleneck of this population.
A Microsatellite-Based Genetic Linkage Map for Channel Catfish, Ictalurus punctatus
Genetics, 2001
Microsatellite loci were identified in channel catfish gene sequences or random clones from a small insert genomic DNA library. Outbred populations of channel catfish contained an average of eight alleles per locus and an average heterozygosity of 0.70. A genetic linkage map of the channel catfish genome (N = 29) was constructed from two reference families. A total of 293 microsatellite loci were polymorphic in one or both families, with an average of 171 informative meioses per locus. Nineteen type I loci, 243 type II loci, and one EST were placed in 32 multipoint linkage groups covering 1958 cM. Nine more type II loci were contained in three two-point linkage groups covering 24.5 cM. Twenty-two type II loci remained unlinked. Multipoint linkage groups ranged in size from 11.9 to 110.5 cM with an average intermarker distance of 8.7 cM. Seven microsatellite loci were closely linked with the sex-determining locus. The microsatellite loci and genetic linkage map will increase the effi...
Indonesian Aquaculture Journal, 2011
African catfish, Clarias gariepinus, is one of economically important farmed species in Indonesia. To support the development of aquaculture industry, high genetic quality of both broodstock and seeds is required and breeding program is considered as viable option. Information on genetic variation of the populations being considered to form a base population may give insight toward the appropriate strategy to be implemented in breeding program. This study was aimed to assess genetic variation in farmed populations of catfish in Indonesia using microsatellite markers with special emphasis on their use to develop breeding program. Three populations of farmed catfish, namely Dumbo, Paiton, and Sangkuriang were collected. Fifteen individuals representing each population were screened for microsatellite variability using seven primer sets (cga01, cga02, cga03, cga05, cga06, cga09, cga10). Results found that with exception of two loci (cga01 and cg02) which had a slight increase, the othe...
Genetic analysis of two common carp broodstocks by RAPD and microsatellite markers
Aquaculture, 2003
The whole broodstock of two Hungarian common carp farms-80 and 196 individuals-was analyzed by using random amplified polymorphic DNA (RAPD) assay and microsatellite analysis. Ten polymorphic RAPD markers and four microsatellites were selected to genotype both of the stocks. As expected, microsatellite analysis revealed more detailed information on genetic diversities than RAPD assay. Results obtained with both types of DNA markers showed lack of major differences between the genetic structure of the two stocks: heterozygosity values and allele frequencies were very similar. Dendrograms created from both sets of data did not show grouping of individuals according to stocks. Genotypes from the two stocks were also compared to those from a limited number of samples collected from other hatcheries and two rivers. Allele frequencies in the groups were similar, with the exception of wild carps. An interesting observation was that three private microsatellite alleles were found in the eight wild carp individuals, compared to the seven detected in the rest of the samples tested (372 individuals). D