Identification of Five New Triterpenoids from Ethylacetate Bark Extract of Holoptelea integrifolia (Roxb.) Planch by GC-MS (original) (raw)
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Natural Products Chemistry & Research
Studies on the chemical constituents of the stem bark of Holoptelea integrifolia (Roxb.) Planch have led to the isolation of a triterpenoid, oleanolic acid (1), the structure of the compound was elucidated via advanced spectroscopic techniques including EI (+), IR, 1 H-NMR and 13 C-NMR. Oleanolic acid was isolated first time from the stem bark of H. integrifolia. Crude ethylacetate extract of the stem bark of H. integrifolia has been tested in vitro for its cytotoxicity by MTT assay. This paper is based on isolation and characterization of the chemical constituents.
Isolation and Identification of a New Phytosterol from Holoptelea Integrifolia (Roxb) Planch Leaves
International Journal of Pharmacy and Pharmaceutical Sciences, 2014
Objective: The Plant Holoptelea Intergrifolia (Roxb.) Planch is being used for the treatment of various disorders since time immemorial in the indigenous system of medicine in India. The main objective of the work was to isolate a new phytosterol from petroleum ether extract of Holoptelea Integrifolia leaves using Preparative Thin Layer Chromatography (TLC). Methods: As per ICH guidelines we have Prepared Thin Layer Chromatographic plates for separation of a new phytosterol from Petroleum ether extract of leaves of Holoptelea Intergrifolia (Roxb.) Planch. The mobile phase used for separation of phytosterol consisted ofChloroform: Ethyl acetate, in the volume ratio of 4:6 (v/v), UV, LC/MS, IR and NMR spectral analytical techniques were used for identification and confirmation of structure of a new Phytosterol by Preparative TLC. Results: Preliminary phytochemical analysis of petroleum ether extract of Holoptelea integrifolia leaves showed the presence of steroids, terpenoids...
Holoptelea integrifolia (Ulmaceae) is a versatile medicinal plant used in various indigenous systems of medicine for curing routine healthcare maladies. It is traditionally used in the treatment and prevention of several ailments like leprosy, inflammation, rickets, leucoderma, scabies, rheumatism, ringworm, eczema, malaria, intestinal cancer, and chronic wounds. In vitro and in vivo pharmacological investigations on crude extracts and isolated compounds showed antibacterial, antifungal, analgesic, antioxidant, anti-inflammatory, anthelmintic, antidiabetic, antidiarrhoeal, adaptogenic, anticancer, wound healing, hepatoprotective, larvicidal, antiemetic, CNS depressant, and hypolipidemic activities. Phytochemical analysis showed the presence of terpenoids, sterols, saponins, tannins, proteins, carbohydrates, alkaloids, phenols, flavonoids, glycosides, and quinines. Numerous compounds including Holoptelin-A, Holoptelin-B, friedlin, epifriedlin, 𝛽-amyrin, stigmasterol, 𝛽-sitosterol, 1, 4-napthalenedione, betulin, betulinic acid, hexacosanol, and octacosanol have been identified and isolated from the plant species. The results of several studies indicated that H. integrifolia may be used as an effective therapeutic remedy in the prevention and treatment of various ailments. However, further studies on chemical constituents and their mechanisms in exhibiting certain biological activities are needed. In addition, study on the toxicity of the crude extracts and the compounds isolated from this plant should be assessed to ensure their eligibility to be used as source of modern medicines.
Triterpenoids from the seedpods of Holarrhena curtisii King and Gamble
Phytochemistry Letters, 2015
Two new hydroperoxy pentacyclic triterpenoids, 3b-hydroxy-11a-hydroperoxyolean-12-en-28-oic acid (1) and 3b-hydroxy-11a-hydroperoxyursan-12-en-28-oic acid (2), together with nine known triterpenoids, squalene (3), b-amyrin acetate (4), a-amyrin acetate (5), lupeol acetate (6), lupeol (7), lanosta-7,24-dien-3b-ol (8), cycloeucalenol (9), oleanolic acid (11) and ursolic acid (12), a known phytosterol, 24-methylenepollinastanol (10), and two known flavanols, (-)-catechin (13) and (-)-gallocatechin (14), were isolated from the methanolic extract of the fresh seedpods of Holarrhena curtisii. Their structures were determined by spectroscopic analysis (one and two dimensional nuclear magnetic resonance, high resolution electrospray ionization mass spectrometry and attenuated total reflectance-Fourier transform infrared spectroscopy). All compounds (except squalene) were evaluated for their in vitro a-glucosidase inhibitory activity. Compounds 1,2, 11 and 12, which had a pentacyclic triterpenoid acid skeleton, showed a strong in vitro a-glucosidase inhibitory activity compared to that of the standard control, acarbose.
HPTLC finger printing analysis of the alkaloids from Holoptelea integrifolia (Roxb.) Planch leaves
Journal of Pharmacognosy and Phytochemistry, 2016
Objective: The present study was conducted to identify the Alkaloids from petroleum ether (PEHI) and methanol extracts (MHI) of medicinally and economically useful leaves of Holoptelea integrifolia (Roxb.) Planch using High Performance Thin Layer Chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp. Results: Preliminary phytochemical screening of petroleum ether extract of Holoptelea integrifolia showed the presence of steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins and carbohydrates while methanolic extract of Holoptelea integrifolia showed the presence of steroids, alkaloids, flavonoids, proteins and carbohydrates. HPTLC finger prin...
Two New Tetracyclic Triterpenes from the Heartwood ofAilanthus excelsaRoxb
Chem Biodivers, 2006
Two new dammarane-type triterpenes, ailexcelone and ailexcelol, together with ocotillone, malabaricol, epoxymalabaricol, lupeol, and sitosterol-3-O-b-d-glucoside were isolated from the heartwood of Ailanthus excelsa. The structures of the new compounds were established on the basis of 1D-and 2D-NMR data. Ocotillone, malabaricol and epoxymalabaricol were isolated for the first time from A. excelsa. All of the isolates were tested for their antifungal activity.
Evaluation of Antioxidant Activity of Leaf Extracts of Holoptelea Integrifolia (Roxb) Planch
International Journal of Applied Pharmaceutics, 2014
Holoptelea integrifolia (Roxb.) Planch has been used from long time in traditional medicine. The main objective of the work was to evaluate the antioxidant activity of leaves of Holoptelea integrifolia (Roxb.) Planch. The antioxidant activity of petroleum ether and methanolic extract of Holoptelea integrifolia (HI) leaves was evaluated using Hydroxyl Radical Scavenging Activity and Total Reduction Capability models. Preliminary Phytochemical investigation of the petroleum ether extract (PEHI) of Holoptelea integrifolia leaves reveals the presence of steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins, and carbohydrate while methanolic extract (MHI) of Holoptelea integrifolia showed the presence of steroids, alkaloids, flavonoids, proteins and carbohydrates. In case of Hydroxyl Radical Scavenging Activity both the HI extracts showed dose dependant increase in percent inhibition i. e. hydroxyl radical scavenging activity and thereby showed antioxidant activity h...
Phytochemical Screening of Leaf and Stem Bark of Holoptelea Integrifolia Roxb
Research Reviews Journal of Botanical Sciences, 2013
Over the centuries societies around world have developed their own traditions to make the sense of medicinal plants and their uses for attempt to overcome illness and suffering and enhance the quality of life. Plants have a great potential for producing new drugs of great benefit to mankind & medicinal properties of plants depend upon the presence of the different physiologically active component which lead to the search for new biologically active principles for therapeutic efficacy. The present phytochemical screening has been carried out on fresh mature leaves and Bark of Holoptelea integrifolia (Roxb) Plant for it's different phyto constituents along with chromatographic profile. The results of the study could be useful in diagnostic tool of the identification of the Holoptelea integrifolia (Roxb) Plant and also to establish the quality and purity of this valuable drug.