The prevalence of Salmonella enterica in Spanish feed mills and potential feed-related risk factors for contamination (original) (raw)
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Journal of Food Safety, 2003
A study of the prevalences of Salmonella, Listeria and thermophilic campylobacters in a dedicated commercial poultry feed mill was undertaken. Salmonella was frequently recovered in samples taken in the preheat and postheat treatment areas of the mill with the overall percentage of samples positive found to be 18.8% and 22.6%, respectively. Feed ingredients and dust collected in the preheat treatment locations within the mill were frequently contaminated with Salmonella (11.8% and 33.3% of samples, respectively). High prevalences of Salmonella were also detected in dust samples (24.2%) obtained from the postheat treatment area of the mill and from feed delivery vehicles (57.1%).Listeria was also recovered from samples at pre- and postheat treatment areas within the mill with overall isolation rates of 14.1% and 18.5% observed, respectively. The most frequently observed species of Listeria recovered from samples in both areas within the mill was L. innocua, L. monocytogenes, L. grayi and L. welshimeri.No thermophilic campylobacters were recovered from any of the samples analyzed in the current study.
Journal of Food Protection, 1997
A survey of contamination with Salmonella spp. was done at 11 sampling sites in a poultry slaughter establishment in Spain for a total of 192 samples. Samples included fecal material, utensils, water, and poultry carcasses and livers at several stages of processing. Salmonella incidence rates increased from 30% in fecal material collected from incoming birds to 60% in air-chilled carcasses and 80% in cold-stored livers, indicating that cross-contamination occurred. The rate of incidence of Salmonella organisms on carcasses averaged 56.7% through post-picking to post-air chilling and reached a maximum of 70% on carcasses at the post-spray wash site. Poultry livers were more heavily contaminated with salmonellae, as 55% and 80% samples after evisceration and cold storage, respectively, were positive for those pathogenic bacteria. From a total of 112 strains isolated, 87 (77.6%) were S. enteritidis, 7 (6.2%) Salmonella serotype 4,5,12:b:-(II), and 6 (5.4%) Salmonella serotype 4,12:b:-(...
Human gastro-enteritis caused by Salmonella enterica is a major health problem in developing countries such as Colombia. In some parts of Colombia, the disease is endemic, and its incidence appears to be increasing, with outbreaks and sporadic cases of diarrhea becoming more frequent. At this time, it is not very clear if either poultry or food is responsible for human salmonellosis contamination in Colombia. The objectives of the present study were to analyze the Pulsed-field gel electrophoresis profiles (PFGEPs) of Salmonella enterica from human patients, poultry and food found in Colombia and to determine the epidemiologic associations between these strains. Twenty-nine isolates of Salmonella enterica subsp. enterica were isolated from: 10 pediatric patients in Bogotá, D.C., 10 different types of food and 9 chickens. All isolates were analyzed by means of the molecular technique XbaI PFGE. Eleven different patterns were observed. These patterns consisted of 12-17 restriction fragments, each with a molecular size of 30-800 kb. The results suggested that Salmonella enterica was transmitted from poultry and food to humans. Surprisingly, among the strains investigated it was impossible to find a direct linkage between poultry and food, indicating, either that Salmonella was incorporated into the food during food processing by handlers, or that foods other than poultry products were the source of human infection. This study about the molecular epidemiology of Salmonella enterica in Colombia provided new information about possible means of human contamination, and should permit health institutions to take adequate measures to avoid sporadic cases and outbreaks of salmonellosis.
2018
Salmonellosis control is in part based on the reduction of Salmonella presence in primary poultry production. The aim of this study was to determine the prevalence of Salmonella in poultry farms of Ecuador. In 2014, the first national survey of industrialized poultry farms was done. Salmonella spp. was isolated from manure samples and serotypes were determined by conventional serotyping. Additionally, the ability of multiplex Polymerase Chain Reaction (PCR) systems to identify the Salmonella serotype of the isolates was evaluated and results were confirmed with serological methods. One hundred forty five farms were sampled and a total of 26 isolates of Salmonella spp. were recovered from 16 of them, resulting in a national apparent prevalence at the farm level of 11%; 12.7% in broiler chickens farms, 11.5% in breeder flocks and 8.9% in egg-laying hens farms. The isolated serotypes of Salmonella enterica subsp. enterica were: Enteritidis, Infantis, Saintpaul and Serogroup O:8:Z4 Comp...