Essential oil and phenolic compounds of Artemisia herba-alba (Asso.): composition, antioxidant, antiacetylcholinesterase and antibacterial activities (original) (raw)
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Biodiversitas, 2022
Artemisia herba-alba Asso (Asteraceae family) is widespread in the semi-arid and arid steppes of North Africa. This plant is used for traditional treatment. The present study aims to investigate the chemical composition, antioxidant and antimicrobial activities of A. herba-alba essential oil found in southwest Algeria (Boussaâda region). Artemisia herba-alba essential oil was obtained by hydrodistillation method, and its chemical composition was identified by using GC/MS analysis. In addition, the antioxidant activity of the extracted essential oil was determined using the DPPH assay. The antimicrobial activity of the essential oil was determined by the agar disc diffusion method. The essential oil extracted from the aboveground portions by hydrodistillation was analyzed by GC/MS. 38 components were identified, making up 69.37% of the oil, the most important of which is thujone (9.875%), camphor (3.762%), cis-pmenthadien-1-of (3.572%), and isoborneol (2.334%). The observed IC50 values of the DPPH assay were 7.31 ± 0.088 mg/mL. On the other hand, this oil was active against all strains tested, this activity varied from 12.77 ± 0.510 mm on Listeria innocua CIP 74915. These results demonstrate that the plant tested could be a potential source of natural antioxidants and antimicrobial agents.
International Journal of Biological Chemistry, 2015
Extraction of essential oils from locally available plant Artemisia Herba-alba was carried out using steam-distillation method. Extracted oils were screened for their chemical composition, antibacterial and antioxidant activities in order to find new metabolite products, which are characterized by a biological activity. Thirty-three constituents, representing 97.54% of the essential oil of Artemisia herba-alba was determined by GC-MS analysis. The main compounds identified are: davanone (42.8%), camphor (15.96%) and thujone (9.63%). The antimicrobial activity of the oil was tested using the agar disc diffusion method, by determining the inhibition zone and the minimum inhibitory concentration. The results have shown a great potential of the antimicrobial activity against the tested strains with an enhanced sensitivity towards the gram-negative strains of Salmonella enteric, Klebsiella and the gram-positive strains of Listeria monocytogenes and Staphylococcus sp. in comparison with the other tested bacteria. The test's results of the essential oil's antioxidant activity, obtained with the anti-radical method 2,2-diphenyl-1-1-picrylhydrazil (DPPH) and the iron-reducing power (FRAP), were compared with those of the ascorbic acid; the usual synthetic antioxidant. The comparison have demonstrated, firstly with the DPPH method a considerable level of antioxidant activity of the essential oil (IC 50 = 17.73±0.29 µg mLG 1) but still lower than that found for the synthetic antioxidant that is the ascorbic acid while the opposite occurred with the iron reduction method, with an higher obtained value of EC 50 =12.53±0.25 µg mLG 1 , for the essential oil's antioxidant activity.
Asian Pacific Journal of Tropical Medicine, 2017
Objective To identify the chemical constituents and biological activities of essential oil and crude methanol extract of Artemisia vulgaris (A. vulgaris) and Gaultheria frarantissima (G. fragrantissima). Methods Phytochemical screening, total phenolic and flavonoid content, antibacterial activities, anti-oxidant assay of the crude extract were carried out to identify the biological activities and phytonutrients present in the extract. Furthermore, the chemical constituents present in the essential oil and crude methanol extract were analyzed using gas chromatography mass spectroscopy and high performance liquid chromatography (HPLC) analysis. Results Gas chromatography mass spectroscopy analysis of essential oil from the aerial part of A. vulgaris revealed 24 different compoundsin it. Sabinene (11.29%), β-thujone (19.19%), chrysanthenone (4.48%), camphor (11.89%), borneol (4.44%) and germacrene D (8.42%) were the major compounds. Similarly, leaves of G. fragrantissimacontained methyl salicylate (95%) and asarone (4.64%). Furthermore, methanol extract of leaves of A. vulgaris and G. fragrantissima were found rich in the total flavonoids and phenolic content. HPLC analysis of the methanol extract of leaves A. vulgaris revealed the presence of morin and luteolin, whereas rutin was found as a major flavonoids compound in the leaves of G. fragrantissima. Further, methanol extract of the A. vulgaris and G. fragrantissima showed the highest antioxidant and antibacterial properties compared to the essential oil. Conclusions The HPLC analysis of the methanol extract of A. vulgaris shows the presence of luteolin and morin, whereas G. fragrantissima reveals the presence of rutin and a glycosylated flavonoids. Results reveal that A. vulgaris oil is the rich source of monoterpene and sesqueterpene compounds. Furthermore, A. vulgaris and G. fragrantissima are the rich source of the phenolic and flavonoids compounds and show good antioxidant and antibacterial activity. Keywords Artemisia vulgarisGaultheria fragrantissimaFlavonoidsPolyphenolAntioxidantAntibacterialHPLCGas chromatography mass spectroscopy
International Journal of Biosciences | IJB |, 2022
The main objective of this study is to investigate the chemical composition, antioxidant and antimicrobial activities of the essential oil of Artemisia herba-alba Asso. of southern Algeria. Essential oil of Artemisia herbaalba Asso was extracted by hydrodistillation, and their chemical composition was identified by GC/MS Antioxidant activity of essential oil, has been done by using DPPH assay. The antimicrobial activity of essential oil was realised by the agar disc diffusion method. The essential oil extracted from the aerial parts by hydrodistillation was analysed by GC/MS. 39 constituents, representing 99.3% of the oil, were identified, of which the major ones, Thujone (12,759 %), Camphor (7,751%), Eacalyptol (4,525%), Isoborneol (1,119%). IC50 values observed for DPPH essay were 20,27 ±0,767 mg/ml. On the other hand, this oil was found effective against all tested strains; this activity ranged from 15.67±1.53 mm with Listeria innocua CIP 74915. These results provided evidence that the studied plant might indeed be a potential source of natural antioxidant and antimicrobial agents.
Phytochemical and Antibacterial Activity of The Essential Oil of Artemisia herba-alba from Morocco
2016
The extraction of essential oils from the aerial part of Artemisia herba-alba is obtained by hydrodistillation and analyzed by gas chromatography coupled with mass spectrometry (GC/MS) for determining their chemical composition. Their antibacterial activity was studied in vitro on two bacterial strains: Salmonella typhi and Staphylococcus aureus. The essential oil yields of the studied plant were 0.21 for fresh aerial part and 0.25% for dry aerial part. The major component of the essential oil from dry aerial part of Artemisia herba-alba was the α-terpineol (47.33%), myrtenyl acetate (22.22%) and chrysanthenyl acetate (20.55%). While the major compounds of essential oil from fresh aerial part was the Borneol (35.68%), αterpineol (33.36%), and δ-cadinol (12.07%). The bacterial strains tested were found to be sensitive to essential oils studied and showed a very effective bactericidal activity with minimum inhibitory concentration (MIC) ranging from 0.125 to 0.5 mg/ml.
Essential oil (E.O.) of Artemisia herba-alba Asso. (Asteraceae) known as Shih, growing wild in Hermel (Lebanon) was obtained by hydrodistillation and analyzed by GC and GC-Mass spectrometry (MS). The results were α-Pinene (45.89%) followed by Borneol (11.3%), 1,8-Cineole (10.8%), Terpineol (6.45%), Camphene (3.94%), γ-Terpinene (3.2%), α-Terpinene (2.72%), (+)-4-Carene (2.2%). The antimicrobial activities of E.O. from Artemisia herba-alba Asso were evaluated against five bacteria and two fungi strains by disc diffusion method and microdilution method. Among Gram positive bacteria growths, S. aureus was shown to be more sensitive (90 mm) E. faecalis (25 mm). On the other hand, three different Gram negative bacterial strains were tested,S. enteritidis showed maximum zone of inhibition (40 mm), followed by E. coli (26 mm) and P. aeruginosa (25 mm) respectively. Theinhibition zones displayed by fungalstrains tested were40 mm and 30 mm for C. albicansand A. fumigatus, respectively. The results of the study showed an interesting antimicrobial profile which could provide promising pharmaceutical and economic benefits of the potential use of the plant essential oils. The present investigation supported the traditional use of Artemisia herba-alba in the Lebanese folk medicine as an antimicrobial active representative of the genus Artemisia. Artemisia herba-albaextracts should be further studied for their potential use in preventing / treating diseases cited in the traditional Lebanese medicine.
Phytochemistry, 2008
The chemical composition of essential oils isolated from aerial parts of seven wild sages from Western Canada -Artemisia absinthium L., Artemisia biennis Willd., Artemisia cana Pursh, Artemisia dracunculus L., Artemisia frigida Willd., Artemisia longifolia Nutt. and Artemisia ludoviciana Nutt., was investigated by GC-MS. A total of 110 components were identified accounting for 71.0-98.8% of the oil composition. High contents of 1,8-cineole (21.5-27.6%) and camphor (15.9-37.3%) were found in Artemisia cana, A. frigida, A. longifolia and A. ludoviciana oils. The oil of A. ludoviciana was also characterized by a high content of oxygenated sesquiterpenes with a 5-ethenyltetrahydro-5-methyl-2-furanyl moiety, of which davanone (11.5%) was the main component identified. A. absinthium oil was characterized by high amounts of myrcene (10.8%), trans-thujone (10.1%) and trans-sabinyl acetate (26.4%). A. biennis yielded an oil rich in (Z)beta-ocimene (34.7%), (E)-beta-farnesene (40.0%) and the acetylenes (11.0%) (Z)-and (E)-en-yn-dicycloethers. A. dracunculus oil contained predominantly phenylpropanoids such as methyl chavicol (16.2%) and methyl eugenol (35.8%). Artemisia oils had inhibitory effects on the growth of bacteria (Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis), yeasts (Candida albicans, Cryptococcus neoformans), dermatophytes (Trichophyton rubrum, Microsporum canis, and Microsporum gypseum), Fonsecaea pedrosoi and Aspergillus niger. A. biennis oil was the most active against dermatophytes, Cryptococcus neoformans, Fonsecaea pedrosoi and Aspergillus niger, and A. absinthium oil the most active against Staphylococcus strains. In addition, antioxidant (beta-carotene/linoleate model) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities were determined, and weak activities were found for these oils.
Antibacterial and antioxidant activities of Artemisia annua essential oil
Fitoterapia, 2002
The essential oil of Artemisia annua aerial parts, consisting of camphor (44%), germacrene D (16%), trans-pinocarveol (11%), b-selinene (9%), b-caryophyllene (9%) and artemisia ketone (3%), was screened for its antimicrobial activity. The essential oil remarkably inhibited the growth of tested Gram-positive bacteria Enterococcus hirae and both tested fungi. This oil has shown an antioxidant activity equivalent to 18% of the reference compound (atocopherol). ᮊ
Molecules, 2022
This study investigated the chemical composition, antioxidant and antimicrobial activity of essential oil extracted from Artemisia aragonensis Lam. (EOA). Hydrodistillation was employed to extract EOA. Gas chromatography with flame ionization detection (GC-FID) and gas chromatography-mass spectrometry analyses (GC-MS) were used to determine the phytochemical composition of EOA. Antioxidant potential was examined in vitro by use of three tests: 2.2-diphenyl-1-picrilhidrazil (DPPH), ferric reducing activity power (FRAP) and total antioxidant capacity assay (TAC). Agar diffusion and microdilution bioassays were used to assess antimicrobial activity. GC/MS and GC-FID detected 34 constituents in the studied EOA. The major component was Camphor (24.97%) followed by Borneol (13.20%), 1,8 Cineol (10.88%), and Artemisia alcohol (10.20%). EOA exhibited significant antioxidant activity as measured by DPPH and FRAP assays, with IC50 and EC50 values of 0.034 ± 0.004 and 0.118 ± 0.008 mg/mL, resp...