Isolation and Identification of Biofilm-Forming Staphylococcus Aureus in Commercial Cow Milk Products (original) (raw)
Related papers
International Journal of Sciences: Basic and Applied Research, 2019
Milk and its derivates are considered vehicles of Staphylococcucs aureus infection in human. S.aureus commonly found on the skin and hair as well as in the noses and throats of people and animals. The bacteria are present in up to 25 percent of healthy people and are even more common among those with skin, eye, nose, or throat infection. S. aureus can cause food poisoning when a food handler contaminates food and then the food is not properly stored. Other sources of food contamination include the equipment and surfaces on which food is prepared .These bacteria multiply quickly at room temperature to produce a toxin that causes illness. S.aureus is killed by cooking and pasteurization. Present study was carried out from June 2017 to November 2018 .The aim this investigation was to isolate Staphylococcus aureus from raw cow milk obtained from different parts of Savar, Ashulia, Dhamrai area distract of Dhaka, Bangladesh. A total of 45 milk samples were collected. Milk samples were ...
2016
The study was intended for molecular detection of S. aureus isolated from raw cow's milk. A total of 20 milk samples were collected from different upazila markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying cultural properties on different selective media, biochemical tests, and finally by PCR. Out of 20 samples, 15 (75%) milk samples were found to be positive for S. aureus. S. aureus specific 16S rRNA gene was amplified from all isolates and identified as S. aureus. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the S. aureus isolates were resistant to amoxicillin (100%), erythromycin (73.33%) and tetracycline (73.33%) but sensitive to azithromycin (93.33%), ciprofloxacin (93.33%), gentamicin (100%), norfloxacin (86.67%) and streptomycin (86.67%).
The study was carryout to evaluate the incidence and multidrug resistant of Staphylococcus aureus in different milk samples. Staphylococcus aureus is a major problem of public health which causes a number of human and animal diseases. The main source of infection is contaminated milk. Totally 50 raw and pasteurized milk samples were collected from three different sources such as Goat milk (15), Buffollow milk (25), and pasteurized milk (10). In order to isolate and identify the Staphylococcus aureus from these samples. The collected milk samples were cultured on nutrient agar; the presumptive Staphylococcus colonies were sub-cultured on Mannitol Salt Agar (MSA) and confirmed by using standard Bacteriological methods. Antimicrobial susceptibility pattern of Staphylococcus aureus was done by Kirby-Bauer disk diffusion method using eight antimicrobials. The prevalence of S.aureus was found to be 25 (50%) out of the total samples examined. In addition, the prevalence of S.aureus was 5 (33.3%) from Goat milk, 16 (64%) from Buffalo milk and 4 (40%) from pasteurized milk. S.aureus was more likely to occur in buffalo milk that were poorly managed and treated frequently with antimicrobials. Thus, out of a total of 25 isolates, high resistance rate was observed primarily to Methicillin 25 (100%) followed by Erythromycin15 (60%), Amoxycillin12 (48%), Vancomycin12 (48%), Gentamycin 12 (48%), Tetracycline 11 (44%), Ciprofloxacin 11 (44%), and low level of resistance to Kanamycin 8 (32%). S.aureus became almost resistant to β-lactams and Erythromycin. The virulence characters of Staphylococcus aureus were characterized phenotypically. The rate of positiveness for Protease, Lipase, β-hemolysis, β-lactamase and Slime formation were 48%, 60%, 60%, 44%, 48%.In this study we concluded that the hygiene of milk is poor and resistant strains have contaminated the milk probably during the process of milking and transportation.
Culture and Molecular Detection of Staphylococcus aureus in Dairy Products of Ahwaz
Staphylococcus aureus is one of the most important bacteria that cause food poisoning and disease in humans and herds. Milk and milk products are important part of the human diet also support the growth of pathogenic organisms. The present study was aimed to determine the prevalence of coagulase positive Staphylococcus aureus in different kinds of dairy products of Ahvaz Province. 460 dairy product samples randomly were collected from Ahvaz Province. The samples were analyzed for the presence of S. aureus. Positive samples screened by microbiological tests. DNA extracted from all isolates and the PCR carried out using specific primers for S. aureus. The results indicated that 127 (27.61%) of dairy samples were contaminated by Staphylococcus aureus (curd 24.5%, dough 19%, butter and cream 14.5%, yogurt 12.5%, cheese 10%, milk 5%). High prevalence of Staphylococcus aureus contamination in milk and milk products of Ahvaz suggests that more control measures should be applied during dairy production. The obtained results are useful for designing strategic plans of prevention and control program against Staph. aureus in dairy ecosystem.
Isolation and characterization of Staphylococcus aureus from raw cow milk in Bangladesh
The study was intended to identification and characterization of Staphylococcus aureus isolated from raw cow milk. A total of 47 milk samples were collected from Sheshmore, Shutiakhali and Bangladesh Agricultural University Dairy Farm, Mymensingh. Using bacteriological, biochemical and PCR-based identification schemes, 12 (25.53%) isolates were confirmed as S. aureus. All the isolates showed β-hemolysis on 5% sheep blood agar. S. aureus specific nuc gene (target size 279-bp) was amplified in the cases of all isolates. The isolates were found as resistant to Penicillin (100%), Erythromycin (75%) and Amoxicillin (100%). On the other hand, the isolates were sensitive to Ciprofloxacin (83.33%), Oxacillin (100%), Cloxacillin (100%) and Neomycin (100%). The isolated S. aureus showed increased resistance to broad spectrum antibiotic (e.g., Ciprofloxacin); this situation is alarming for the dairy industry and public health.
The study was carried out from July 2010 to June 2011. The aim of the present investigation was to isolate Staphylococcus aureus from raw milk obtained from cow from different parts of Nagpur district, India and to determine their antibiotic susceptibility profile. General biochemical tests along with the MASTASTAPH TM rapid agglutination tests were employed for bacterial identification. All the well characterized isolates were further confirmed by amplifying coa gene by PCR. A total 95 S. aureus isolates were obtained during this study from 50 raw milk samples. When all the well characterized isolates were subjected to antibiotic susceptibility testing, erythromycin and chloramphenicol were the most effective antibiotics. A large proportion of isolates were found to be resistant to Co-trimoxazole (89%) followed by Linomycin (81%) and Penicillin (35%) It was concluded from the current study that the hygiene of the milk was poor and resistant strains contaminated the milk probably during the process of transportation.
Culture and Molecular Detection of Staphylococus aureus in Dairy Products of Ahwaz
Staphylococus aureus is one of the most important bacteria that cause fod poisoning and disease in humans and herds. Milk and milk products are important part of the human diet also suport the growth of pathogenic organisms. The present study was aimed to determine the prevalence of coagulase positve Staphylococus aureus in diferent kinds of dairy products of Ahvaz Province. 460 dairy product samples randomly were colected from Ahvaz Province. The samples were analyzed for the presence of S. aureus. Positve samples screned by microbiological tests. DNA extracted from al isolates and the PCR caried out using specific primers for S. aureus. The results indicated that 127 (27.61%) of dairy samples were contaminated by Staphylococus aureus (curd 24.5%, dough 19%, buter and cream 14.5%, yogurt 12.5%, chese 10%, milk 5%). High prevalence of Staphylococus aureus contamination in milk and milk products of Ahvaz sugests that more control measures should be aplied during dairy production. The obtained results are useful for designing strategic plans of prevention and control program against Staph. aureus in dairy ecosystem. Keyword: Staphylococus aureus, dairy products, Culture, PCR.
2017
Sufficient evidence exists to effect that milk is an excellent source of nutrients for both man (Sharma and Joshi, 1992) and bacteria (Henry and Newlander, 1997). It is therefore required that milk be consumed only after it is pasteurized to make it safe. Unfortunately, recent evidence has revealed postpasteurization contamination of milk with pathogenic bacteria (Oliver et al., 2005) and antibiotic resistant bacteria. According to Hawkey (2008) antimicrobial resistance is currently the greatest challenge to the effective treatment of infectious globally. Contamination of milk with microbes therefore results in rapid multiplication and this may deteriorate the quality of milk leading to issues of food safety (Frazier and West Hoff, 1986) and public health concern when the microbes are antibiotic resistant (Guerra et al., 2003). Milk is a nutritious food for human beings, acting as good medium for the growth of many microorganisms, International Journal of Current Microbiology and Ap...
Journal of Nutrition & Food Sciences
This investigation was carried out from October 2011-June 2012 to determine the prevalence of Staphylococcus in milk and rate of contamination at farm and milk distribution tank in Alage Agricultural Technical Vocational Educational Training College. Milk samples (row) were cultured on sheep blood agar and incubated. The plates were examined for gross colony morphology, pigmentation and haemolytic characteristics at 24-48 h then presumptive colonies of Staphylococcus aureus was selected and sub cultured on nutrient agar and incubated. Then, bacteria were identified according to its Gram reaction, morphology and the catalase test, tube coagulase test (4 h), haemolysis, pigment production (golden yellow), mannitol and maltose fermentation were used. A total of 170 milk sample of cow were examined for bacteriological status of S. aureus, an overall 28.2% prevalence of Staphylococcus was found. From this, 21.2% was directly from the farm and 35.3% were from milk distributing site to consumers. The total prevalence of Staphylococcus varied among the sample taking site. The prevalence of S. aureus from distributing raw milk site (35.3%) was significantly higher than the prevalence of S. aureus from samples taken directly from the farm (21.2%). From this the study it is considered that Staphylococcosis a potential hazard for the public and contamination rate is high in distribution site which needs improvement of the hygienic status of the personnel's at distribution site.
PREVALENCE OF STAPHYLCOCCUS AUREUS IN RAW MILK: CAN IT BE A POTENTIAL PUBLIC HEALTH THREAT
Background:Staphylococcus aureus is important milk borne pathogen and causes a wide variety of diseases in humans and animals and it is frequently associated with subclinical mastitis in dairy animals and may contaminate milk and other dairy products which act as vehicles for S. aureus infection in humans. Aim: The present study was aimed to identify the prevalence of S. aureus in raw milk samples from small holder dairy units in Chennai city and to relate the results with public health implication of the infection levels in dairy animals and the milk. Methodology: A total of 100 individual raw milk samples (50 cows, 10 buffaloes, 40 goats) were collected. Standard protocol was followed for the isolation of S. aureus from milk in Baird Parker Agar with 2% egg yolk tellurite emulsion. Colonies suggestive for S. aureus (black surrounded by white halo) were submitted for the morphological characteristics by Gram's staining and biochemical tests such as catalase and coagulase tests for confirmation. The positive presumptive isolates were further confirmed by Polymerase Chain Reaction (PCR) by targeting Nuc gene. Results: The overall prevalence rate of S. aureus in milk samples based on conventional techniques was 61 per cent (cow's milk 68%, goat's milk 62.5% and buffaloes milk 40%) and 65.57% S. aureus presumptive isolates were positive by PCR which includes 73.53 % of cow's milk, 52 % of goat's milk and 50% of buffaloes milk. Conclusion: In this study examined raw milk samples contains S. aureus indicates a potential route of S. aureus transmission may occur to consumers via contaminated milk or through contaminated dairy products. Hence, to improve the quality of milk and to prevent S. aureus contamination more hygienic preventive measures should be implemented.