Assessment of serum biomarkers of oxidative stress in patients with chronic renal failure (original) (raw)
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The evaluation of oxidative stress in patients with chronic renal failure
Clinica Chimica Acta, 2002
Background: Free radical-mediated changes are thought to be involved with atherosclerosis in patients with chronic renal failure. Methods: The protein carbonyl and malondialdehyde (MDA) levels in serum as the markers of radical-induced protein and lipid oxidations were measured in chronic renal failure patients. Results: Serum carbonyl and MDA levels in both hemodialysis and peritoneal dialysis patients were not found to be different as compared with healthy subjects. In both patient groups, the approximately twofold increment in total antioxidant activity (ferric reducing/antioxidant power; FRAP) and uric acid values in serum were found. The high uric acid levels in both patient groups might be partly responsible for the increment in FRAP values. In addition, all patients received multivitamin preparations including ascorbate, which was also a major antioxidant in serum. Conclusions: Our data suggest that oxidative stress does not become the major threat for patients with chronic renal failure. The increment in endogenous and exogenous antioxidant capacities in serum might be thought to prevent any possible radical-induced damage in patients with chronic renal failure. In addition, the increased nitric oxide (NO) levels especially in hemodialysis patients might likely favor an antioxidant effect. D
The association of oxidant-antioxidant status in patients with chronic renal failure
Renal failure, 2015
Oxidative stress has been linked to disease progression, including chronic renal failure (CRF). The aim of the present study was to determine malondialdehyde (MDA) as a sign of lipid peroxidation, and to investigate the association between antioxidant activities and three trace elements, in 49 patients with CRF. The erythrocyte and plasma trace elements [selenium (Se), zinc (Zn), and copper (Cu)] and antioxidant defense levels were determined: glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), vitamins E and C. The obtained values were compared with 42 age- and sex-matched healthy controls. There were significantly lower mean values of plasma Se, GPx, vitamins E and C, erythrocyte Se, SOD and CAT levels in the patient group compared to the control group (p < 0.001). Plasma MDA showed a significant increase in all CRF patients in comparison with controls. No significant difference was found in plasma Cu, Zn, and erythrocyte GPx, Cu and Zn levels between pati...
Biochemistry Research International, 2013
Oxidative stress seems to be involved in the path physiology of cardiovascular complications of chronic kidney disease (CKD). In this study, we determined the effect of different stages of CKD and substitutive therapies on oxidative stress. One hundred sixtyseven patients (age: 44 ± 06 years; male/female: 76/91) with CKD were divided into 6 groups according to the National Kidney Foundation classification. Prooxidant status was assessed by assaying thiobarbituric acid reactive substances, hydroperoxides, and protein carbonyls. Antioxidant defence was performed by analysis of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, vitamin E, Iron, and bilirubin. TBARS and LPO were higher in HD patients compared to other groups ( < 0.001), while protein carbonyls were more increased in PD patients. The antioxidant enzymes were declined already at severe stage of CKD and they were declined notably in HD patients ( < 0.001). Similar observation was found for vitamin E, Fe, and bilirubin where we observed a significant decrease in the majority of study groups, especially in HD patients ( < 0.001). The evolution of CKD was associated with elevated OS. HD accentuates lipid, while PD aggravates protein oxidation. However, the activity of antioxidant enzymes was altered by impaired renal function and by both dialysis treatments.
https://www.ijrrjournal.com/IJRR\_Vol.5\_Issue.12\_Dec2018/Abstract\_IJRR0035.html, 2018
Background: Chronic Kidney Disease (CKD) is one of the major diseases claiming a sizeable fraction of overall mortality and morbidity every year in our country. Multiple studies have found increased oxidative stress and imbalance in antioxidant defense in CKD patients. Apart from the disease pathology itself, other associated factors, like inflammation, hemodialysis etc. also contribute to increased oxidative stress, which in turn complicate the clinical condition further. There are multitude of markers to measure oxidative stress and antioxidant defense, but it is very difficult to measure overall oxidative stress status accurately and completely. There is also very little data available about the oxidative stress status in CKD patients in the population of West Bengal. Aims and Objectives: This study aims to measure Total oxidative Stress (TOS) and Total antioxidant defense (TAD) status in CKD patients in a sample population of West Bengal, more efficiently, by a colorimetric method modified and standardized in our laboratory. We also aim to find out the relationship (if any) of the Oxidative stress status in these patients with the severity of the disease Materials and Methods: The study included 40 CKD patients from OPD and IPD of a tertiary care hospital of Kolkata, West Bengal, and 30 age and sex matched healthy volunteers. Serum Urea, Creatinine, Sodium and Potassium were measured by standardized commercial kits and TOS and TAD were measured by colorimetric chemical methods, modified and standardized in our laboratory. Results: The TOS was found to be significantly increased (P=0.003) in CKD patients in comparison to healthy control subjects. TAD was also found to be significantly elevated (P<0.001) in the patient group. However, TOS and TAD showed no correlation with serum creatinine (P>0.05) in our study group. Conclusion: The total oxidative stress does indeed increase in the chronic Kidney Disease, in the population of West Bengal too, and this result reaffirms the similar findings elsewhere in the world. The increase in Total Antioxidant Defense in our study population, contrary to a usual decrease in TAD found with increase in TOS, indicates a compensatory increase as found and suggested by some previous studies. This study did not find any significant relationship of TOS and TAD levels with disease severity, which warrants a more elaborate study with a larger sample size, and taking into account different confounding factors.
Research Square (Research Square), 2022
Introduction: Oxidative stress is de ned as an imbalance between oxidant production and antioxidant defense mechanisms, in favor of oxidant production. Oxidative stress has been implicated in the pathogenesis and development of chronic kidney disease (CKD). Hemodialysis (HD) has also been described to contribute remarkably to oxidative stress in CKD patients. This study aims to evaluate the levels of different oxidative stress biomarkers in Syrian patients with chronic kidney disease (CKD) that also analyzed the effect of HD on redox status. Materials and Methods: Blood samples were collected from CKD (n=30) and HD (n=30) patients and healthy individuals (n=30), and the following markers of oxidative stress were analyzed: malondialdehyde (MDA), advanced oxidation protein product (AOPP), glutathione (GSH), in addition to Hgb, Hct, CRP and Cr. Results: We have found that the levels of MDA, AOPP, CRP, and Cr in CKD and HD groups were signi cantly higher than in the control group (P=0.0001, P=0.003, P=0.0001, and P=0.0001 respectively). Also, we have found that the levels of GSH, Hgb, and Hct in CKD and HD groups were signi cantly lower than in the control group (P=0.0001, P=0.0001and P=0.0001, respectively). On the other hand, there were no statistically signi cant differences (P>0.05) in MDA, AOPP, GSH, and CRP between CKD and HD groups whilst there were statistically signi cant differences (P<0.05) in Hgb, Hct, and Cr between CKD and HD groups. Conclusions: CKD and HD patients have both higher concentrations of all studied serum oxidative stress biomarkers and decreased concentrations of antioxidants. Also, the results showed that the greatest in uence on the development of oxidative stress is the severity of renal failure rather than Hemodialysis treatment.
Kidney International, 2004
Background. Cardiovascular disease is known to be the most important complication among patients with renal failure, and oxidative stress has been proposed to play a major role as the source of such complications. Human serum albumin (HSA) is composed of human mercaptoalbumin (HMA) with cysteine residues having reducing powers, of reversibly oxidized human non-mercaptoalbumin-1 (HNA-1), and strongly oxidized human non-mercaptoalbumin-2 (HNA-2).
F1000Research
Background: Several aspects of chronic kidney disease (CKD) such as the incidence rate and mortality rate are concerning. Oxidative stress contributes to progression and mortality in patients with CKD; however, a specific correlation between several markers of oxidative stress and the estimated glomerular filtration rate (eGFR) and albumin-creatinine ratio (ACR) in the Indonesian population has not been sufficiently described yet. Methods: This study was an analytic observational study with a sample of 56 patients with CKD in Universitas Airlangga Hospital, Surabaya, Indonesia, from December 2019 – March 2020. The markers for oxidative stress investigated were urinary 8-hydroxy-2 deoxyguanosine (8-OHdG), serum symmetric dimethylarginine (SDMA) and asymmetric dimethylarginine (ADMA). The correlations between each variable of oxidative stress and CKD were analyzed using Pearson analysis. Results: There was a positive correlation between 8-OHdG and eGFR (p=0.00, r=0.51); however, there...
Oxidative stress in primary glomerular diseases: a comparative study
Molecular and Cellular Biochemistry, 2008
Objective To evaluate the status of oxidative stress in patients with different primary glomerular diseases (PGD) which have differential predisposition to renal failure. Methods Seventy-three patients with PGD and 50 controls were enrolled in the study. They were sub-grouped into non-proliferative glomerulonephritis (NPGN) and proliferative glomerulonephritis (PGN). Levels of serum malondialdehyde (MDA), reactive nitrogen intermediates (RNI), plasma total homocysteine (tHcy), urine 8-isoprostane (8-IP), RBC thiols, glutathione-S-transferase (GST) and serum superoxide dismutase (SOD) were measured spectrophotometrically. Results PGD patients showed a significant increase in MDA, RNI, tHcy, 8-IP levels (P \ 0.05) and decreased SOD, total thiols and protein bound thiol levels as compared to controls (P \ 0.05). Significantly higher levels of tHcy, MDA and 8-IP (P \ 0.05) and lower SOD enzyme activity (P \ 0.05) were observed in PGN group as compared to NPGN and control groups. These changes remained significant even after adjustment was made for creatinine. Conclusions Oxidative stress in PGN is significantly higher than NPGN, indicating higher oxidative stress in these patients, independent of degree of renal dysfunction.