Polymorphisms of the Myostatin gene (MSTN) and its associationwith growth traits in Bali cattle (original) (raw)
Related papers
Media Peternakan, 2016
Myostatin (MSTN) gene plays a key role in skletal muscle homeostasis such as inducing muscle athrophy, poliferation of myoblast, increasing ubiquitin-proteasomal, downregulating IGF pathway, and glucolysis. Myostatin gene expression is controled by CpG island located in promoter region. The objectives of this research were to identify polymorphism of MSTN promoter gene and to associate the polymorphism of SNP with growth and muscling traits in Bali cattle. A total of 48 Bali cattle from BPTU-HMT Bali island was screened to identify genetic polymorphisms in MSTN promoter region using sequencing method. The growth and muscling traits were measured at 12 months of age. The muscling traits were evaluated using ultrasound console with linear transducer having frequency 6.5 Hz and scaning we conducted at 130 mm in deep. Analysis of polymorphism was conducted by using PopGen 1.32 software. The association of MSTN with growth and muscling traits were analyzed by using General Linear Model (GLM) procedure. This result showed that a total of 20 polymorphic SNPs (seven SNPs in CpG island) were detected in this region. Although, only 3 SNPs (g.-8078C>T, g.-7996G>C, and g.-7930A>G) had equilibrium condition in Hardy-Weinberg analysis. The association result showed that 2 SNPs (g.-7799T>C and g.-7941C>T) were significantly associated with intramuscular fat percentage (P≤0.05) in Bali cattle. Although the 2 SNPs were nominally significant at nominal P≤0.05 threshold, they were not significant after Bonferroni correction for multiple testing. It could be concluded that MSTN promoter gene was polymorphic in Bali cattle and there were 2 SNPs associated with carcass quality.
Simultaneous genotyping to detect myostatin gene polymorphism in beef cattle breeds
Journal of Animal Breeding and Genetics, 2002
SummaryThe myostatin gene codes for a growth factor involved in muscle development, and polymorphism in this gene can have important economic consequences. Nine mutations affecting the amino‐acidic sequence have already been described, six of which are disruptive, inactivating the protein and causing bovine muscular hypertrophy. As the number of known mutations grows, it is necessary to develop a simple, routinely usable technique able to screen individuals in all populations. The oligonucleotide ligation assay (OLA) is proposed here for the rapid genotyping of the nine mutations known affecting the coding sequence in the main breeds of beef cattle. This technique showed its ability to reveal the genotype of individuals being a good tool to determine the frequency of each mutation in a population. The procedure is very flexible as new mutations can be added and removed at any time. Depending on the genotype of each individual, the technique allows breeders to make quick decisions on...
Expression of Myostatin Gene in Belgian Blue and Ongole Grade Crossbred Cattle
Buletin Peternakan, 2022
Investigating Myostatin (MSTN) as a potent inhibitor of skeletal muscle growth and development to produce excessive muscles is extremely essential for livestock breeding. This study aimed to analyze the expression of the MSTN gene and its relationships with genotype and phenotype (normal-muscled vs double-muscled) of Belgian Blue (BB) x Ongole Grade (PO) crossbred cattle. For that purpose, 12 animals from BB, PO, BB x PO F1, and BB x PO F2 cattle (3 animals each) raised at Balai Embrio Ternak (BET) Cipelang Bogor, West Java were used for blood sample collection. Genotyping analysis was performed using the PCR-RFLP method withprimer F: 5’-CTC TTC TTT CCT TTC CAT ACA GAC-3’ and R: 5’-AGG GGA AGA CCT TCC ATG TT-3’, while the MSTN gene expression was analyzed using the qPCR technique. As results, three genotypes: del.11/del.11, +/del.11, and +/+ were detected. The del.11/del.11 genotype, which showed a double-muscled phenotype was found in BB cattle and BB x PO F2 cattle. The +/del.11 g...
Veterinary World, 2021
Background and Aim: Myostatin (MSTN), a member of the transforming growth factor-β family, is a negative regulator of muscle mass. This study aimed to detect the genetic variation of the 1160 bp fragment of exon 1 and part of intron 1 of the MSTN gene in several cattle populations raised in Indonesia. Materials and Methods: Polymerase chain reaction products of the MSTN gene amplified from 92 animals representing 10 cattle populations (Peranakan Ongole [PO], Belgian Blue x PO cross, Rambon, PO x Bali cross, Jabres, Galekan, Sragen, Donggala, Madura, and Bali) were sequenced, compared, and aligned with bovine MSTN of Bos taurus (GenBank Acc. No. AF320998.1) and Bos indicus (GenBank Acc. No. AY794986.1). Results: Four nucleotide substitutions (nt 1045 and 1066 in intron 1; nt 262 and 418 in exon 1) and two indels (nt 807 and 869 in intron 1) were synonymous mutations. Among these substitutions, only the nt 262G>C and nt 418A>G loci were polymorphic in all populations, except Bali cattle. The frequencies of the nt 262C (0.82) and nt 418A (0.65) alleles were highest. For the nt 262G>C locus, the CC genotype had the highest frequency (0.66) followed by GC (0.30) and CC (0.03). For the nt 418A>G locus, the AG genotype had the highest frequency (0.52) followed by AA (0.39) and GG (0.09). Conclusion: The results, showing genetic variations in exon 1 and intron 1 of the MSTN gene, might be helpful for future association studies.
Myostatin Gene Analysis in the First Generation of the Belgian Blue Cattle in Indonesia
Journal of the Indonesian Tropical Animal Agriculture, 2016
Telah dilakukan suatu penelitian untuk mengidentifikasi variasi gen myostatin dan mengevaluasi keberadaan marker genetik untuk sifat double muscling (delesi 11 basa pada ekson 3 gen myostatin) pada generasi pertama (F1) hasil persilangan dengan sapi Belgian Blue pertama di Indonesia. Sebanyak 8 sampel DNA sapi yang dipelihara di PT. Karya Anugerah Rumpin (KAR) digunakan dalam penelitian ini. Analisa Single Strand Conformation Polymorphism (SSCP) dan sekuensing digunakan untuk mengidentifikasi keragaman dari gen myostatin. Terdapat 3 tipe alel gen myostatin berdasarkan hasil analisa SSCP. Pejantan Belgian Blue memiliki tipe alel A. Sapi Simmental, Wagyu, SO x BX, Charolais, dan sapi PO memiliki tipe alel B. Sementara itu anak sapi hasil perkawinan Belgian Blue x FH dan Belgian Blue x SO memiliki tipe alel C (heterozigot). Berdasarkan hasil analisa sekuen gen myostatin, ditemukan adanya delesi 11 basa pada ekson 3 gen myostatin pejantan Belgian Blue. Hasil analisa sekuensing juga memberikan informasi bahwa generasi F1 (Belgian Blue x FH dan Belgian Blue x SO) memiliki gen myostatin yang berada dalam kondisi heterozigot. Hasil penelitian ini memberikan bukti ilmiah bahwa delesi 11 basa pada ekson 3 gen myostatin yang merupakan marker untuk sifat double muscling pada sapi Belgian Blue juga ada (diwariskan) pada anak-anak sapi generasi F1 (jantan dan betina).
Genetic polymorphism of myostatin (MSTN) in Nigerian sheep breeds
Journal of Animal Science and Veterinary Medicine, 2021
Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) has been implicated to play an important role in growth regulation, and it is a candidate gene in marker assisted selection (MAS). This study was carried out to identify the polymorphism of MSTN gene as a genetic marker for growth traits in Nigerian indigenous sheep. Genomic DNA (gDNA) was extracted from blood samples of Balami, Yankasa, Uda and West African Dwarf (WAD) breeds of sheep. Parts of 5’UTR, intron and exon1 (614bp) was amplified using a primer sequence designed by FastPCR-primer software. The amplicons were digested with restriction enzyme HaeIII and the fragments produced were stained with luminescent dye and run on gel electrophoresis. The genetic structure of the sampled population was investigated after analysis with POPGENE32 software. The HaeIII digested results showed that Myostatin has three polymorphs (AA, AB and BB), controlled by two alleles (A and B), with B having a higher allelic frequen...
Journal of microbiology, biotechnology and food sciences, 2019
Marker assisted selection (MAS) is a potential tool to genetically enhance the traits of economic importance in livestock production. One of the gateways to MAS is the identification of SNPs for the desired genes. The aim of the present study was to detect SNPs in myostatin (MSTN) gene which has a negative role in growth and skeletal muscle development. Therefore, polymorphisms of the exon no 1, 2 and 3 of MSTN gene were studied in Thalli (n=10) and Pak-karakul (n=10) sheep breeds. The genomic DNA was isolated and amplified by using PCR. The amplicons were purified and sequenced. After PCR all samples were sent to Singapore for 1st base laboratory for further purification and sequencing. Sequencing analysis was done using Codon Code Aligner and MEGA 6 software’s. Mutations were observed at only exon no 1 in 50% (5/10) Thalli sheep (heterozygous G>T =3/5, synonymous =2/5) and 20% (2/10) Pak-karakul sheep (only synonymous T mutations) at 3995bp. The two alleles (A and B) were found...
Genetics and Molecular Research, 2012
Myostatin, a transforming growth factor-beta superfamily member, has been well documented as a negative regulator of muscle growth and development. Myostatin, which has 376 amino acids, is synthesized as a precursor protein. Polymorphism of the myostatin gene in Makoei sheep was investigated by PCR and single-strand conformation polymorphism technique (SSCP). Genomic DNA of 92 sheep was isolated from whole blood. A 417-bp myostatin intron I segment was amplified by standard PCR, using locus-specific primers. Four SSCP patterns, representing four different genotypes, were identified. The frequencies of the genotypes were 0.413, 0.293, 0.130, and 0.163 for AD, AC, AE, and BC, respectively. Allele frequencies were 0.4185, 0.0815, 0.2283, 0.2065, and 0.0652 for A, B, C, D, and E, respectively. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (4): 3568-3575 (2012) Polymorphisms in the ovine myostatin gene 3569
Status of the F94L mutation of the myostatin gene in cattle breeds in Indonesia
AIP Conference Proceedings
A missense F94L mutation of the bovine myostatin gene (MSTN-F94L) has been used as an alternative marker for increasing meat yield without any severe problems primarily on reproduction traits in beef cattle. The mutant A allele of MSTN-F94L is considered to be exclusive in the Limousin breed. In our previous study, the wild-type C allele of MSTN-F94L was found to be fixed in Bali, Sumbawa, and Holstein-Friesian cattle. However, we found four of 168 individuals of Pasundan cattle carrying the mutant A allele. Therefore, to confirm the status of the mutant A allele of MSTN-F94L mutation in other cattle breeds in Indonesia, a total of 388 bulls representing 5 cattle breeds (Limousin, Simmental, Holstein-Friesian, Bali and Peranakan Ongole) from two National Artificial Insemination Centers (NAICs) of Indonesia were used in this study. All bulls were genotyped using PCR-RFLP methods. We found that the mutant A allele was only present in Limousin bulls with high frequency (94%). The frequency of CC, CA, and AA genotypes in Limousin bulls were 0.9, 9.3, and 89.7%, respectively. In contrast, no mutant A allele was found in the other breeds. This study showed that the mutant A allele of MSTN-F94L mutation may originate from Limousin bulls. The origin of the mutant A allele in Pasundan cattle may be derived from the Limousin bulls of NAICs through artificial insemination (AI). Limousin bulls are the only bull breeds as a source of the mutant A allele of MSTN-F94L in NAICs.
animal, 2009
Despite many recent advances in single nucleotide polymorphism (SNP) genotyping technologies, there is still the need for methodologies with a reasonable throughput. In this study, we compared three PCR-based methods for SNP detection: (1) a conventional PCR-based allele detection system with fluorescent genotyping technology, (2) a SNaPshot methodology by single nucleotide primer extension and, (3) a real-time PCR-based method by allele-specific minor groove-binder probes. These three methodologies were used to analyze 104 meat samples of a particular Italian cattle breed known for producing excellent quality meat and for a characteristic increased development of muscle mass, caused by a point mutation (C313Y) in theMyostatingene. The analysis revealed 98 samples to be homozygous (mh/mh) and five to be heterozygous (mh/+) for the mutation whereas one sample resulted to be homozygous for the wild type (+/+). The results obtained with the three different assays were consistent. Overa...