Hyperthermia, Cytotoxicity, and Cellular Uptake Properties of Manganese and Zinc Ferrite Magnetic Nanoparticles Synthesized by a Polyol-Mediated Process (original) (raw)
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Pharmaceutics, 2021
The clinical translation of magnetic hyperthermia (MH) needs magnetic nanoparticles (MNPs) with enhanced heating properties and good biocompatibility. Many studies were devoted lately to the increase in the heating power of iron oxide MNPs by doping the magnetite structure with divalent cations. A series of MNPs with variable Zn/Fe molar ratios (between 1/10 and 1/1) were synthesized by using a high-temperature polyol method, and their physical properties were studied with different techniques (Transmission Electron Microscopy, X-ray diffraction, Fourier Transform Infrared Spectroscopy). At low Zn doping (Zn/Fe ratio 1/10), a significant increase in the saturation magnetization (90 e.m.u./g as compared to 83 e.m.u./g for their undoped counterparts) was obtained. The MNPs’ hyperthermia properties were assessed in alternating magnetic fields up to 65 kA/m at a frequency of 355 kHz, revealing specific absorption rates of up to 820 W/g. The Zn ferrite MNPs showed good biocompatibility a...
In-vitro application of Mn-ferrite nanoparticles as novel magnetic hyperthermia agents
Journal of Materials Chemistry B 2 8390 (2014)
Manganese ferrite nanoparticles were synthesized by a facile, low-cost, environmentally friendly and high yield methodology based on the aqueous co-precipitation of proper salts. Firstly, structural, morphological and magnetic characterization schemes were performed to dete rmine crucial factors for optimizing their heating potential, such as size, polydispersity, saturation magnetization and coercivity. In an effort to simulate the in vivo environment of animal tissue phantoms and study the thermal heating effects resulting from Brownian motion and hysteresis losses, nanoparticles at various concentrations were embedded in aqueous media of varying agar concentration. During the in vitro application healthy cells (primary bone marrow-derived osteoblasts and 3T3-L1 fibroblast-like preadipocytes) and human osteosarcoma Saos-2 cells, were incubated with manganese ferrite nanoparticles. The heating profile of the particles was studied in different concentrations and in correlation with their potential cytotoxic effect. Our results revealed concentration dependent cytotoxicity profile and uptake efficiency together with variable specific loss power values yet with fast thermal response, opening novel pathways in material selection as hyperthermia agents.
Small versus Large Iron Oxide Magnetic Nanoparticles: Hyperthermia and Cell Uptake Properties
Molecules, 2016
Efficient use of magnetic hyperthermia in clinical cancer treatment requires biocompatible magnetic nanoparticles (MNPs), with improved heating capabilities. Small (~34 nm) and large (~270 nm) Fe 3 O 4-MNPs were synthesized by means of a polyol method in polyethylene-glycol (PEG) and ethylene-glycol (EG), respectively. They were systematically investigated by means of X-ray diffraction, transmission electron microscopy and vibration sample magnetometry. Hyperthermia measurements showed that Specific Absorption Rate (SAR) dependence on the external alternating magnetic field amplitude (up to 65 kA/m, 355 kHz) presented a sigmoidal shape, with remarkable SAR saturation values of~1400 W/g MNP for the small monocrystalline MNPs and only 400 W/g MNP for the large polycrystalline MNPs, in water. SAR values were slightly reduced in cell culture media, but decreased one order of magnitude in highly viscous PEG1000. Toxicity assays performed on four cell lines revealed almost no toxicity for the small MNPs and a very small level of toxicity for the large MNPs, up to a concentration of 0.2 mg/mL. Cellular uptake experiments revealed that both MNPs penetrated the cells through endocytosis, in a time dependent manner and escaped the endosomes with a faster kinetics for large MNPs. Biodegradation of large MNPs inside cells involved an all-or-nothing mechanism.
Chemistry of Materials, 2010
Stoichiometric Mn 0.2 Zn 0.8 Fe 2 O 4 monodisperse nanoparticles were prepared by the so-called polyol method. The variation of magnetization as a function of magnetic field H (up to (50 kOe) and temperature (5-320 K) were investigated, for zero-field-cooled (ZFC) and field-cooled (FC) conditions on freshly produced powder. The T variation of the low-field (H = 200 Oe) magnetic susceptibility is characteristic of superparamagnets with a blocking temperature below room temperature. The H variation of the low temperature (T = 5 K) magnetization exhibits a hysteresis loop. The coercivity is weak, about 0.2-0.3 kOe, which is typical of soft-ferrimagnetic materials. The 0 K saturation magnetization and the Curie temperature are found to be 98 emu.g-1 and 360 K, respectively. The magnetic properties of the particles are discussed in relation with their chemical composition and their cation distribution in the opportunity of using them as heating mediators for hyperthermia application in cancer therapy. In this aim, the toxicity of the particles was also evaluated by viability assays on human umbilical vein endothelial cells (HUVEC).
Pharmaceutics
We report the synthesis of magnetite nanoparticles (IOMNPs) using the polyol method performed at elevated temperature (300 °C) and high pressure. The ferromagnetic polyhedral IOMNPs exhibited high saturation magnetizations at room temperature (83 emu/g) and a maximum specific absorption rate (SAR) of 2400 W/gFe in water. The uniform dispersion of IOMNPs in solid matrix led to a monotonous increase of SAR maximum (3600 W/gFe) as the concentration decreased. Cytotoxicity studies on two cell lines (cancer and normal) using Alamar Blues and Neutral Red assays revealed insignificant toxicity of the IOMNPs on the cells up to a concentration of 1000 μg/mL. The cells internalized the IOMNPs inside lysosomes in a dose-dependent manner, with higher amounts of IOMNPs in cancer cells. Intracellular hyperthermia experiments revealed a significant increase in the macroscopic temperatures of the IOMNPs loaded cell suspensions, which depend on the amount of internalized IOMNPs and the alternating m...
RSC Adv., 2015
Cobalt zinc ferrite (CZF) magnetic nanoparticles (MNPs) were synthesized by modifying a thermal decomposition method in the presence of triethylene glycol (TEG). Initially structural, morphological, and magnetic characterizations were carried out in order to confirm their size, polydispersity, colloidal stability, and magnetic property. Fourier transform infrared spectroscopy (FTIR) confirmed the presence of triethylene glycol (TEG) on the surface of CZF MNPs. The CZF MNPs has revealed a superparamagnetic nature with high saturation magnetization, good colloidal stability, high specific absorption rate (SAR), excellent biocompatibility, and a monodispersed nature. All these properties are crucial, for their use as a nanomedicine in magnetic fluid hyperthermia (MFH) treatment; which is considered to be one of the most promising cancer therapies. The prepared CZF MNPs are found to be biocompatible with MCF7 (human breast cancer) and L929 (mouse fibroblast) cell lines, when tested by MTT and SRB assays. Cell particle interaction was examined in depth, by using multiple staining techniques coupled with confocal microscopy. Finally, an in vitro hyperthermia experiment was carried out on MCF7 cells, resulting in the extinction of MCF7 cells by up to 80% within 60 min. The nature of the cell extinction was found and lastly reactive oxygen species (ROS) production was assessed, where ROS is the responsible factor for apoptosis. This research demonstrates that, prepared CZF MNPs can be used as a potential candidate for effective MFH treatment for cancer cell extinction. Fig. 5 Biocompatibility and cell particle interaction study on L929 cell line. (a) Cell viability study by using MTT assay, for 24 h and 48 h. (b)
Central European Journal of Chemistry, 2013
In the current work, iron oxide magnetic nanoparticles (MNP’s) were synthesized by thermal decomposition of Fe(acac)3-(iron acetylacetonate) compounds in high-boiling organic solvents containing stabilizing surfactants and examined as possible agents for magnetic hyperthermia treatment, according to their structural, magnetic and heating properties. Three different cancer cell lines (DA3, MCF-7 and HeLa cell lines) were used to assess the suitability of the MNP’s. The experimental results proved that the synthesized MNPs are non-toxic and the uptake efficiency was extremely good. Further, from in vitro hyperthermia results, very fast thermal response was observed (reaching hyperthermia levels in less than 200 s), which minimize the duration of the cell and human body exposure in a high frequency AC external magnetic field.
Journal of Materials Science: Materials in Medicine, 2015
Superparamagnetic nanoferrites are prepared by simple and one step refluxing in polyol synthesis. The ferrite nanoparticles prepared by this method exhibit particle sizes below 10 nm and high degree of crystallinity. These ferrite nanoparticles are compared by means of their magnetic properties, induction heating and cell viability studies for its application in magnetic fluid hyperthermia. Out of all studied nanoparticles in present work, only ZnFe 2 O 4 and CoFe 2 O 4 MNPs are able to produce threshold hyperthermia temperature. This rise in temperature is discussed in detail in view of their magneto-structural properties. Therefore ZnFe 2 O 4 and CoFe 2 O 4 MNPs with improved stability, magnetic induction heating and cell viability are suitable candidates for magnetic hyperthermia.