Eicosapentaenoic acid-rich biomass production by the microalga in a continuous-flow reactor (original) (raw)
Related papers
Lipid production by Phaeodactylum tricornutum
Bioresource Technology, 1991
The marine diatom Phaeodactylum tricornutum Bohlin is a potential source of the pharmaceutically valuable w3 polyunsaturated fatty acid eicosapentaenoic acid (EPA). The results of indoor continuous growth of Phaeodactylum tricornutum Bohlin are reported.
Enzyme and Microbial Technology, 2000
A low expense process is developed for recovering esterified eicosapentaenoic acid (EPA) from microalgae and fish oil. Over 70% of the EPA content in the esterified crude extract of microalgae were recovered at purities exceeding 90%. The recovery scheme utilizes either wet or freeze-dried algal biomass. The process consists of only three main steps: 1) simultaneous extraction and transesterification of the algal biomass; 2) argentated silica gel column chromatography of the crude extract; and 3) removal of pigments by a second column chromatographic step. Argentated silica gel chromatography recovered about 70% of the EPA ester present in the crude fatty ester mixture of fish oil, but at a reduced purity (ϳ83% pure) compared to the microalgal derived EPA. The optimal loading of the fatty ester mixture on the chromatographic support was about 3% (w/w) but loadings up to 4% did not affect the resolution significantly. The process was scaled up by a factor of nearly 320 by increasing the diameter of the chromatography columns. The elution velocity remained constant. Compared to the green alga Monodus subterraneus, the diatom Phaeodactylum tricornutum had important advantages as a potential commercial producer of EPA. For a microalgal EPA process to be competitive with fish oil derived EPA, P. tricornutum biomass (2.5% w/w EPA) needs to be obtained at less than $4/kg. If the EPA content in the alga are increased to 3.5%, the biomass may command a somewhat higher price. The quality of microalgal EPA compares favorably with that of the fish oil product. Compared to free fatty acid, EPA ester is more stable in storage. Shelf-life is extended by storing in hexane. The silver contamination in the final purified EPA was negligibly small (Ͻ210 ppb).
Production of 13 C polyunsaturated fatty acids from the microalga Phaeodactylum tricornutum
Journal of Applied Phycology, 2000
An integrated process for the indoor production of 13 C labelled PUFA from Phaeodactylum tricornutum is presented. The core of the process is a bubble column photobioreactor from which the exhaust gas from the reactor is returned to the culture by a low pressure compressor. To avoid accumulation of dissolved oxygen in the culture medium, the exhaust gas is bubbled through a sodium sulphite solution before returning it to the reactor. Carbon is removed from the medium before inoculating the alga, then labelled 13 CO 2 is injected for pH control and carbon supply. The reactor has been operated in semicontinuous mode at a dilution rate of 0.01 h −1 , a biomass productivity of 0.1 g L −1 d −1 being obtained. Under this conditions both pH and dissolved oxygen were correctly controlled and the adequacy of the system for autotrophic production of labelled biomass was demonstrated. Analysis by GC-MS revealed that the fatty acids content of the biomass obtained was 10% d.wt., the content of eicosapentaenoic acid was 2.5% d.wt. All the fatty acids were labelled, more that 90% of the carbon present in these fatty acids was 13 C. Element analysis of biomass and supernatant showed that 59.5% of injected carbon was assimilated into the biomass whereas 33% remained in the supernatant, and 7.5% remained undetected. Due to the high cost of 13 CO 2 different strategies for the optimisation of labelled carbon use are proposed.
Journal of applied microbiology , 2022
Utilization of sustainable natural resources such as microalgae has been considered for the production of biofuels, aquaculture feed, high-value bioactives such as omega-3 fatty acids, carotenoids, etc. Eicosapentaenoic acid (EPA) is an omega-3 fatty acid present in fish oil, which is of physiological importance to both humans and fish. Marine microalgae are sustainable sources of lipid rich in EPA, and differ
Eicosapentaenoic acid (20∶5n-3) from the marine microalgaPhaeodactylum tricornutum
Journal of the American Oil Chemists’ Society, 1996
Eicosapentaenoic acid (EPA, 20:5n-3) was obtained from the marine microalgae Phaeodactylum tricornutum by a three-step process: fatty acid extraction by direct saponification of biomass, polyunsaturated fatty acid (PUFA) concentration by formation of urea inclusion compounds, and EPA isolation by semipreparative high-performance liquid chromatography (HPLC). Alternatively, EPA was obtained by a similar two-step process without the PUFA concentration step by the urea method. Direct saponification of biomass was carried out with two solvents that contained KOH for lipid saponification. An increase in yield was obtained because the problems associated with emulsion formation were avoided by separating the biomass from the soap solution before adding hexane for extraction of insaponifiables. The most efficient solvent, ethanol (96%) at 60~ for 1 h, extracted 98.3% of EPA. PUFA were concentrated by the urea method with a urea/fatty acid ratio of 4:1 at a crystallization temperature of 28~ and by using methanol and ethanol as urea solvents. An EPA concentration ratio of 1.73 (55.2:31.9) and a recovery yield of 78.6% were obtained with methanol as the urea solvent. This PUFA concentrate was used to obtain 93.4% pure EPA by semipreparative HPLC with a reverse-phase, C18, 10 mm i.d. x 25-cm column and methanol/water (1% acetic acid), 80:20 w/w, as the mobile phase. Eighty-five percent of EPA loaded was recovered, and 65.7% of EPA present in P. tricomutum biomass was recovered in highly pure form by this three-step downstream process. Alternatively, 93.6% pure EPA was isolated from the fatty acid extract (without the PUFA concentration step) with 100% EPA recovery yield. This two-step process increases the overall EPA yield to 98.3%, but it is only possible to obtain 20% as much EPA as that obtained by three-step downstream processing.
Chemical engineering transactions, 2019
Microalgal biomass has been considered a potential source of lipids, proteins, and carbohydrates for different industrial applications. Usually, molecules with a possibility of use in food, pharmaceutical, and cosmetic industry are added-value products that can make microalgae large-scale production economically viable. Examples of molecules present in microalgae biomass are triglycerides rich in Polyunsaturated Fatty Acids (PUFAs) widely studied due to the benefits to human health, including prevention of cardiovascular diseases and diabetes. Some marine microalgae such as Isochrysis sp., Nannochloropsis sp. and diatom Phaeodactylum tricornutum are known to exhibit high quantities of long-chain PUFA such as Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA). In addition, some species show a high content of Monounsaturated Fatty Acids (MUFAs) on lipids, as Botryococcus braunii, which usually contains more than 50% of oleic acid. Other high-value molecules from microalgae bio...
Biotechnology and Bioengineering
Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen availability affect productivity, oil yield, and fatty acid (FA) composition of P. tricornutum UTEX 640. After preliminary laboratory trials, outdoor experiments were carried out in 40-L GWP 1 reactors under different nitrogen regimes in batch. Nitrogen replete cultures achieved the highest productivity of biomass (about 18 g m À2 d À1) and EPA (about 0.35 g m À2 d À1), whereas nitrogen-starved cultures achieved the highest FA productivity (about 2.6 g m À2 d À1). The annual potential yield of P. tricornutum grown outdoors in GWP 1 reactors is 730 kg of EPA per hectare under nutrient-replete conditions and 5,800 kg of FA per hectare under nitrogen starvation.
Potential Production of Polyunsaturated Fatty Acids from Microalgae
2016
Abstract- Currently, public awareness of healthcare importance increase. Polyunsaturated fatty acid is an essential nutrition for us, such arachidonic acid, docosahexaenoic acid and eicosapentaenoic acid. The need of Polyunsaturated fatty acid generally derived from fish oil, but fish oil has a high risk chemical contamination. Microalgae are single cell microorganism, one of Phaeodactylum tricornutum which have relatively high content of eicosapentaenoic acid (29,8%). Biotechnology market of Polyunsaturated fatty acid is very promising for both foods and feeds, because the availability of abundant raw materials and suitable to develop in the tropics. This literature review discusses about the content of Polyunsaturated fatty acid in microalgae, omega-3, omega-6, Polyunsaturated fatty acid production processes, and applications in public health.
Marine Drugs
Polyunsaturated fatty acids (PUFAs) are essential for human function, however they have to be provided through the diet. As their production from fish oil is environmentally unsustainable, there is demand for new sources of PUFAs. The aim of the present work was to establish the microalgal platform to produce nutraceutical-value PUFAs from forest biomass. To this end, the growth of Phaeodactylum tricornutum on birch and spruce hydrolysates was compared to autotrophic cultivation and glucose synthetic media. Total lipid generated by P. tricornutum grown mixotrophically on glucose, birch, and spruce hydrolysates was 1.21, 1.26, and 1.29 g/L, respectively. The highest eicosapentaenoic acid (EPA) production (256 mg/L) and productivity (19.69 mg/L/d) were observed on spruce hydrolysates. These values were considerably higher than those obtained from the cultivation without glucose (79.80 mg/L and 6.14 mg/L/d, respectively) and also from the photoautotrophic cultivation (26.86 mg/L and 2....