044. False Positives Anti-Neutrophil Cytoplasmic Antibodies by Indirect Immunofluorescence in the Immunology Laboratory Routine (original) (raw)
depending on the site and size of the involved blood vessels. The inability to predict which patients will suffer from disease relapses is the current biggest unmet need in the context of vasculitis. Beside this, long-term immunosuppressive treatments with heavy side effects are the mainstay of treatment for all patients. Within this context, an international effort is ongoing under the name of RELENT with the ambition to identify new candidate biomarkers to tailor vasculitis patientś treatment. As part of this project, our aim is to profile the autoimmune repertoire in vasculitis patients and controls. Methods: Screening was performed using planar and bead-based antigen arrays generated using protein fragments from the Human Protein Atlas collection (www.proteinatlas.org). Over three years, more than 500 serum samples from small-vessel ANCA-associated vasculitis (AAV), large-vessel vasculitis giant cell artheritis (GCA), polymyalgia rheumatica (PMR), and healthy controls were collected and screened for their reactivity towards nearly 2,000 antigens representing 1,520 unique human proteins. Results: An untargeted screening on planar antigen arrays identified 84 reactive antigens that were selected for further analysis. Targets published in the context of vasculitis were also added to this selection, reaching a total of 371 protein fragments, representing 240 unique protein IDs. Fragments were coupled on magnetic beads to create a bead-array that was applied to test sera from vasculitis patients and controls. Data analysis identified antigens showing different patterns of reactivity across different conditions, subgroups, and timepoints. Conclusion: The application of high-multiplex and high-throughput antigen array technologies allowed us to provide new insights in the autoimmune repertoire.
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