Hexon and fiber gene changes in an attenuated fowl adenovirus isolate of Malaysia in chickens embryonated eggs and its infectivity in chickens (original) (raw)
2018, Journal of veterinary science
Fowl adenovirus (FAdV) is distributed worldwide and caused economic losses in poultry industry. The objectives of this study were to determine the hexon and fiber gene changes in an attenuated FAdV isolate of Malaysia in specific pathogen free (SPF) chicken embryonated eggs (CEE) and its infectivity in commercial broiler chickens. SPF CEE was inoculated with 0.1mL FAdV inoculum via chorioallantoic membrane (CAM) for 20 consecutive passages. The isolate at passage 20 (E20) with virus titer of 10TCID/mL was inoculated (0.5mL) into day old commercial broiler chickens either via oral or intraperitoneal route. The study demonstrated that 100% embryonic mortality was recorded from passage 2 (E2) to E20 with delayed pattern at passage 17 onwards. The lesions were confined in the liver and CAM. Substitutions of amino acids in L1 loop of hexon at position 49 and 66, and in knob of fiber gene at position 318 and 322 were recorded in the E20 isolate. The isolate is belong to serotype 8b and no...
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