Determination of Curcuminoids in Curcuma longa Linn. by UPLC/Q-TOF-MS: An Application in Turmeric Cultivation (original) (raw)
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The action of turmeric depends on three curcuminoids: curcumin, demethoxycurcumin and bisdemethoxycurcumin, whose distribution is highly varied among cultivars. A sensitive method for estimation of all three curcuminoids is essential for quality control. We developed a HPLC-MS method with lowest limits of detection and quantification of curcuminoids. Mass spectrometry (MS) authenticated the identity of curcuminoids. Principal component analysis of curcuminoids established the high variation among the selected seven cultivars of Curcuma, as well as commercial powders. We suggest that our HPLC method can be used for quality control of turmeric.
Curcuminoid in Curcuma longa extract
Analysis of individual and total curcuminoid composed of curcumin (CUR), desmethoxycurcumin (DMCUR) and bisdemethoxycurcumin (BDMCUR) in Curcuma species is very important. Curcuminoids are frequently used as markers in herbal medicine involving the use of Curcuma species in its formulation. The objective of this study was to validate high-performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy-partial least square (FTIR-PLS) for quantitative analysis of total and individual curcuminoids in turmeric (Curcuma longa L.) extract. The turmeric powder was macerated using ethanol and evaporated to obtain an ethanolic extract. The actual contents of individual curcuminoids of CUR, DMCUR, and BDMCUR were determined using HPLC. FTIR-PLS can be used for quantitative analysis of individual and total curcuminoids using specific wavenumbers. The coefficient of determination (R 2 ) for the relationship between actual values and FTIR-PLS predicted values for calibration, internal, and external validation was >0.98 which indicated good accuracy. The relatively low values of errors in calibration, validation and external validation indicated good precision. FTIR spectroscopy-PLS can be used as an alternative technique over HPLC for determination of individual and total curcuminoid in turmeric extracts.
Molecules, 2018
A high-performance liquid chromatography (HPLC) method was investigated for the simultaneous quantification of two chemical types of bioactive compounds in the rhizome of Curcuma longa Linn. (turmeric), including three curcuminoids: Curcumin, bisdemethoxycurcumin, and demethoxycurcumin; and three volatile components: ar-turmerone, β-turmerone, and α-turmerone. In the present study, the sample extraction system was optimized by a pressurized liquid extraction (PLE) process for further HPLC analysis. The established HPLC analysis conditions were achieved using a Zorbax SB-C18 column (250 mm × 4.6 mm i.d., 5 µm) and a gradient mobile phase comprised of acetonitrile and 0.4% (v/v) aqueous acetic acid with an eluting rate of 1.0 mL/min. The curcuminoids and volatile components were detected at 430 nm and 240 nm, respectively. Moreover, the method was validated in terms of linearity, sensitivity, precision, stability and accuracy. The validated method was successfully applied to evaluate the quality of twelve commercial turmeric samples.
Research Journal of Phytochemistry, 2015
A simple and isocratic liquid chromatographic using UV detection at wavelength of 425 nm have been validated and used for quantitative analysis of curcumin in ethanolic extract of turmeric (Curcuma longa Linn.) and Curcuma xanthorriza (Zingiberaceae), indigenous to Java region, Indonesia. The method was optimized for separation of three curcuminoids namely curcumin, demethoxycurcumin and bisdemethoxycurcumin using Waters Xterra MS C18 column (4.6×250 mm, 5 μm). The mobile phase used consisted of aquabidestilata and acetonitrile (65:35 v/v) containing 1% acetic acid. The analytical method was validated in terms of linearity, sensitivity, precision and accuracy. The developed method was linear over the curcumin concentration range of 10-60 μg mLG 1 with correlation coefficient value of 0.999. The precision of the developed method expressed as Relative Standard Deviation (RSD) value was in the range of 0.17-1.17% for three different levels of sample. The recoveries obtained for the accuracy assessment were 8.50-101.23%. The sensitivity of analytical method was expressed with Limit of Detection (LoD) and Limit of Quantification (LoQ). The values of LoD and LoQ were 1.13 and 3.34 μg mLG 1 , respectively. The method was successfully used for quantitative analysis of curcumin in the rhizomes of Curcuma longa and Curcuma xanthorriza. The levels of curcumin found in those rhizomes are in the range of 3.03±0.01-7.31±0.02 (C. longa) and in the range of 1.69±0.02-4.92±0.01 (C. xanthorriza).
Asian Pacific Journal of Tropical Biomedicine, 2012
India Objective: To develop a simple, sensitive, precise, and accurate stability-indicating high performance thin-layer chromatographic method for analysis of curcumin (the main active constituent of turmeric). Methods: The separation was achieved on TLC aluminum plates precoated with silica gel 60F 254 using toluene-chloroform-methanol (5:4:1, v/v/v) as a mobile phase. Densitometric analysis was performed at 430 nm. Results: This system was found to have compact spot of curcumin at RF value of (0.31暲0.02). For the proposed procedure, linearity (r2 = 0.99354 暲 0.00120), limit of detection (50 ng/spot), limit of quantification (200 ng/spot), recovery (ranging from 98.35%-100.68%), and precision (曑2.25%) were found to be satisfactory. Statistical analysis reveals that the content of curcumin in different geographical region varied significantly. Conclusions: The highest and lowest concentration of curcumin in Turmeric was found to be present in sample of Erode (Tamilnadu) and Surat (Gujrat) respectively which inferred that the variety of turmeric found in Erode (Tamilnadu) is much superior to other region of India.
Occurrence of curcuminoids in Curcuma longa : A quality standardization by HPTLC
Bangladesh Journal of Pharmacology, 2008
A simple high performance thin layer chromatographic (HPTLC) method has been developed for the simultaneous determination of the pharmacologically important active curcuminoids viz. curcumin, demethoxycurcumin and bis-demethoxycurcumin in Curcuma longa L. The assay combines the separation and quantification of the analytes on silica gel 60 GF 254 HPTLC plates with visualization under UV and scanning at 425 nm. Using this technique, the alkaloidal content of different parts of the title plant has been determined.
Journal of Pharmacognosy and Phytochemistry, 2018
Curcuma longa (Turmeric) is used successfully in Ayurvedic formulations from ancient times. It is a rich source of bioactive phytoconstituents like curcuminoids, turmerone and many more. Curcuminoids is the group of chief dynamic components and has number of medicinal uses such as anti-inflammatory, anti-HIV, antitumour, antiviral, anticancer, antifungal and antiparasitic. Different analytical methods have been developed in recent year for the quality control analysis of curcuminoids in Curcuma longa extract including HPLC, HPTLC and UV-Visible Spectrophotometry. While the primary component curcumin from curcuminoids is still lacking for its analytical method development along with validation. Therefore, in the present study, a simple UV visible and HPLC method was developed and validated according to international conference harmonization (ICH) guidelines for the quantitative estimation of curcumin in Curcuma longa extract.
A rapid and simple high performance thin layer chromatographic method has been developed for the simultaneous quantitation of pharmacologically important diaryl heptanoids curcumin, demethoxy curcumin, and bis-demethoxy curcumin in Curcuma longa and C. amada. The assay combines the isolation and separation of curcuminoids on silica gel 60F 254 high performance thin layer chromatographic plates, followed by scanning of the spots at 366 nm using a UV detection mode.