Influence of pristine SWNTs in supramolecular hydrogelation: scaffold for superior peroxidase activity of cytochrome c (original) (raw)

2012, Chemical Communications

Materials: Cytochrome c (oxidized), all amino acids, palmitic acid, dicyclohexylcarbodiimide (DCC), 4-N,N-(dimethyl)aminopyridine (DMAP), 1hydroxybenzotriazole (HOBT) and all solvents were purchased from SRL India. 1,1´carbonyl diimidazole (CDI), thionyl chloride, sodium hydroxide were purchased from Spectrochem, India. Pyrogallol was obtained from Qualigens Fine chemical Company, India. Hydrogen peroxide (30%, w/v solution) was purchased from Ranbaxy, India. Single walled carbon nanotubes (SWNT, 1-2 nm diameter) and all deuteriated solvents for NMR experiments were obtained from Aldrich Chemical Co. Milli-Q Water was used throughout the study. Thin layer chromatography was performed on Merck precoated silica gel 60-F 254 plates. 1 H NMR spectra were recorded in AVANCE 300 MHz (Bruker) spectrometer. Mass spectrometric data were acquired by electron spray ionization (ESI) technique on a Q-tofmicro quadruple mass spectrometer (Micromass). Elemental analyses were performed on Perkin Elmer 2400 CHN analyzer. Probe sonication was done using Omni Sonic Ruptor 250. Bath sonication was performed with a Telsonic Ultrasonics bath sonicator. Sorvall RC 6 was used for centrifugation respectively. Synthetic procedure: All dipeptide amphiphiles were synthesized following the reaction conditions as reported previously. 1 Briefly, methyl ester of protected L-amino acid was

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