Variability of IgE reactivity profiles among European mite allergic patients (original) (raw)
Related papers
PubMed, 2006
Several studies have shown that the presence of IgE antibodies to house dust mites (HDM), particularly Dermatophagoides pteronyssinus (Dpt), is an important risk factor for asthma. Allergen immunotherapy is indicated for patients with IgE antibodies to clinically relevant allergens. The aims of this study were to analyze the levels of specific serum IgE to Der p 1 and Der p 2 allergens in mite-sensitized atopic patients and to compare them with both in vivo (skin prick test) and in vitro (IgE-ELISA) sensitizations to Dpt crude extract. Forty-seven atopic patients with allergic rhinitis with or without intermittent or persistent mild asthma and positive skin prick test (SPT) to Dpt total extract were studied. Thirty age-matched healthy subjects with negative SPT to HDM were included as controls. Levels of total IgE and Dpt-, Der p 1- and Der p 2-specific IgE were measured by ELISAs in SPT-positive atopic patients and SPT-negative control subjects. Among 47 symptomatic atopic patients, 27 (57.4%) were double positive IgE to Der p 1 and Der p 2 allergens, 3 (6.4%) were single positive IgE to Der p 1, 4 (8.5%) were single positive IgE to Der p 2, and 13 (27.6%) were double negative IgE to both allergens. There was a significant correlation between Der p 1- and Der p 2-specific IgE levels, but not between Der p 1- or Der p 2-IgE levels and SPT results. The double negative IgE patients had the smallest skin test reactions although they showed high mean levels of total serum IgE. Therefore, the knowledge of specific IgE levels to Der p 1 and Der p 2 major allergens might support physicians for indication or follow-up in mite-sensitized patients under allergen-specific immunotherapy. These approaches might be important for obtaining improved safety and efficacy of the current clinical practice of allergen immunotherapy.
Clinical & Experimental Allergy, 2012
Background Commercially available house dust mite components may improve routine testing of allergic patients. Objective To establish the prevalence and serum levels of IgE to commercial Der p 1, Der p 2, Der p 10 and the carbohydrate MUXF3 in house dust-mite allergic patients. To compare individual vs. allergen microarray methods. Methods Prevalence and serum levels of IgE to Dermatophagoides pteronyssinus extract and components Der p 1, Der p 2, Der p 10 and MUXF3, specific IgG 4 to D. pteronyssinus, total serum IgE levels, and clinical features (age, asthma, rhinitis and atopic dermatitis) were determined in 123 patients (64 children) with the ImmunoCAP ® method. Immuno-CAP ISAC ® was performed in 24 patients. Results All patients had serum IgE to D. pteronyssinus. Prevalences of serum IgE to commercial components were Der p 1 93%, Der p 2 77% (Der p 1 or Der p 2 94%), Der p 10 28% and MUXF3 25%. Levels of D. pteronyssinus IgE strongly correlated with Der p 1 and Der p 2 IgE (r = 0.89 and 0.85 respectively), but not Der p 10 and MUXF3. Immuno-CAP ® and ImmunoCAP ISAC ® were concordant, but the quantitative correlation was poor. No clinical implication for the prevalence, levels, or molecular IgE reactivity profile to house dust mite components was found. Conclusions and Clinical Relevance Commercially available Der p 1 and Der p 2 strongly correlate with IgE D. pteronyssinus. The lack of Der p 1 and Der p 2 IgE may help with differential diagnosis. Der p 10 serum IgE prevalence and levels suggest different patterns in food and mite-related tropomyosin sensitization. Serum IgE to carbohydrate MUXF3, although unexpectedly prevalent, were low and did not modify D. pteronyssinus IgE levels. Follow-up may be best carried out with individual rather than microarrayed components.
Depiction of Major Mite Allergens in Severe Allergic Rhinitis with High Mite Perennial Exposure
Turkish Archives of Otorhinolaryngology, 2020
Objective: Airway diseases, including allergic rhinitis, are prompted by specific IgE antibodies against airborne allergens and notably those derived from mites. The presented study focused on the specific IgE immediate response to Dermatophagoides pteronyssinus (D. pteronyssinus) mite major allergens and the corresponding pertinence of molecular diagnosis in patients bothered with severe persistent rhinitis. Methods: Individuals exhibiting confirmed sensitization to D. pteronyssinus along with a clinical diagnosis of coexisting severe allergic rhinitis were included in the study. In vivo investigations encompassed intradermal testing with a battery of standardized allergenic extracts, concurrent with in vitro specific IgE to the crude extract of D. pteronyssinus, and associated individual molecular allergens were assessed. Results: Fifty-five out of 59 subjects showed sero-dominant IgE responses to the major allergens Der p 1, Der p 2 and Der p 23. Both Der p 2 and Der p 23 reached a prevalence above 80%, while group 10 allergen tropomyosin was scarcely depicted (<10%) and exclusively present in younger individuals. Conclusion: The proposed component-resolved diagnosis panel accurately recognized 93.22% of D. pteronyssinus allergic patients. The raised seroprevalence of IgE response to Der p 23 also confirmed this constituent as a major D. pteronyssinus allergen in severe allergic rhinitis. A molecular approach appears to be essential to frame a more precise diagnosis and eventually a personalized immunotherapy regarding this allergic condition.
House Dust Mite Allergy and the Der p1 Conundrum: A Literature Review and Case Series
Allergies
The house dust mite (HDM) is globally ubiquitous in human habitats. Thirty-two allergens for Dermatophagoides farinae and 21 for Dermatophagoides pteronyssinus have been detected so far. The present minireview summarizes information about the role of Der p 1 as a key coordinator of the HDM-induced allergic response and reports on a series of Italian patients who are allergic to HDMs. We studied the specific IgE profiles in a population of patients with allergic asthma and rhinitis screened for specific immunotherapy (SIT) for HDM allergies, with the aim of obtaining insights into the pathogenic role of Der p1. Patients co-sensitized to other airborne allergens showed a higher prevalence of asthma (9/12 (75%) vs. 2/7 (29%); p < 0.05) than did HDM mono-sensitized patients. The latter group showed higher Der p1 concentrations than that of the co-sensitized group (p = 0.0360), and a direct correlation between Der p1 and Der p2 (r = 0.93; p = 0.0003) was observed. In conclusion, our s...
International Archives of Allergy and Immunology, 2016
sensitized to Der p 23, and 11 patients were negative for all HDM MeDALL chip components. Seven sera were available for further testing, and 3 of them showed IgE reactivity to dot-blotted nDer p 1, and 2 reacted with high-molecular weight components (>100 kDa) in nitrocellulose-blotted HDM extract when tested with 125 I-labeled anti-IgE in a RASTbased assay. The HDM extract-specific IgE levels of the 11 patients were <3.9 kU/l. Conclusions: Recombinant allergen-based IgE serology is of great value when conventional IgE diagnostics fails. Der p 23 is an important HDM allergen, especially when major allergens are negative. Therefore, it would be desirable to have Der p 23 commercially available. Further research concerning the prevalence and clinical significance of different HDM allergens is needed.
Journal of Allergy and Clinical Immunology, 1998
Background: The use of allergen extracts will hamper studies into quantitative aspects of allergic responses because the precise amount of relevant allergen for each patient is unknown. Objective: We applied isolated IgE-binding components (major allergens) in the technique of bronchial allergen challenge to determine the role of patient characteristics in the early asthmatic response (EAR). Methods: In 30 patients with mild-to-moderate asthma, the EAR was investigated after inhalation of an isolated major allergen of Dermatophagoides pteronyssinus (i.e., Der p 1 [n = 16] or Der p 2 [n = 14]). The degree of early-phase bronchial responsiveness to allergen (the cumulated dose of allergen causing a 20% fall in FEV 1 [PD 20 allergen]) was related to the degree of nonspecific bronchial responsiveness (the concentration of histamine causing a 20% fall in FEV 1 [PC 20 histamine]) and the level of specific IgE or allergen thresholds as found in skin tests and basophil histamine release assays. Results: Twenty-seven patients with an immediate response during allergen and histamine challenges (∆FEV 1 , ≥20%) were analyzed. In these patients, a strong correlation was found between PD 20 allergen and PC 20 histamine (r = 0.81, p < 0.001). Weak correlations were found between PD 20 allergen and the level of specific IgE (r = -0.36, p = 0.07) or allergen thresholds as found in skin tests (skin prick test, r = 0.36 and p = 0.07; intracutaneous test, r = 0.49 and p = 0.01) or basophil histamine release assays (r = 0.37, p = 0.08). Moreover, no significant contribution of these indices of IgE-mediated hypersensitivity to the prediction of PD 20 allergen by multilinear regression models with PC 20 histamine was found. Conclusion: In asthmatic patients allergic to house dust mites the degree of nonspecific bronchial hyperresponsiveness is the main determinant of early-phase bronchial responsiveness to allergen. In these patients the degree of allergic sensitivity does not contribute to the prediction of the EAR after allergen inhalation. (J Allergy Clin Immunol 1998;102:24-31.)
Quantitative determination of Der p I allergen levels in allergenic extracts and house-dust samples
Aerobiologia, 1992
House-dust mites are responsible for serious respiratory diseases in humans. An ELISA assay to detect Dermatophagoides pteronyssinus (Dp) mites has been set up. This assay, based on quantitative determination of the major Dp allergen, called Der p I, has been applied for the analysis of growhzg mite cultures and house-dust samples. Der p I allergen levels in mite cultures are well correlated with the number of mites present as well as with the biological activity of the corresponding extracts. Different methods for detecting Dp mites in house-dust samples were compared. The ELISA method shows good sensitivity and specificity, and is particularly suitable for routine analyses.
Standardization of House Dust Mite Allergen (A13 D. Pteronyssinus )
Biotechnology & Biotechnological Equipment, 2007
The introduction of new in vitro methods, recommended by the European pharmacopoeia (1), enrich the characterization and evaluation of the allergens. The standardization of house dust mite allergen (A13 Dermatophagoides pteronyssinus) is based on different methods for determination of its structure and biological activity. Five batches of allergen were standardized by determination of protein nitrogen, by isoelectric focusing and by assessment of major allergen Der p1 by ELISA. The biological activity of the A13 was evaluated by skin prick test (SPT), IgE-binding potential after competitive inhibition and its activation capabilities for in vitro basophil degranulation. These batches, produced from 2002 -2006, were with identical protein distribution according their isoelectrical points. Der p1 and protein nitrogen quantities varied between 37 -87 ng/ml and 39 -34 mg %. The studied lots showed similar results in the IgE-binding properties in comparison with the reference at 50 % inhibition level and in fl ow cytometry test for allergen-specifi c basophil degranulation. All the batches were with similar biological activity and induced skin-allergic reactions, comparable with the in-house reference preparation and histamine hydrochloride standard 1 mg/ml. The data from these new, in vitro methods improve the results from the accepted standardization procedure and will be performed as routine in the evaluation of biological activity of the house dust mite and others allergens.