A Genetic Screen Reveals an Unexpected Role for Yorkie Signaling in JAK/STAT-Dependent Hematopoietic Malignancies in Drosophila melanogaster (original) (raw)

2017, G3: Genes|Genomes|Genetics

A gain-of-function mutation in the tyrosine kinase JAK2 (JAK2 V617F) causes human myeloproliferative neoplasms (MPNs). These patients present with high numbers of myeloid lineage cells and have numerous complications. Since current MPN therapies are not curative, there is a need to find new regulators and targets of Janus kinase/Signal transducer and activator of transcription (JAK/STAT) signaling that may represent additional clinical interventions. Drosophila melanogaster offers a low complexity model to study MPNs as JAK/STAT signaling is simplified with only one JAK [Hopscotch (Hop)] and one STAT (Stat92E). hop Tumorous-lethal (Tum-l) is a gain-of-function mutation that causes dramatic expansion of myeloid cells, which then form lethal melanotic tumors. Through an F1 deficiency (Df) screen, we identified 11 suppressors and 35 enhancers of melanotic tumors in hop Tum-l animals. Dfs that uncover the Hippo (Hpo) pathway genes expanded (ex) and warts (wts) strongly enhanced the hop Tum-l tumor burden, as did mutations in ex, wts, and other Hpo pathway genes. Target genes of the Hpo pathway effector Yorkie (Yki) were significantly upregulated in hop Tum-l blood cells, indicating that Yki signaling was increased. Ectopic hematopoietic activation of Yki in otherwise wild-type animals increased hemocyte proliferation but did not induce melanotic tumors. However, hematopoietic depletion of Yki significantly reduced the hop Tum-l tumor burden, demonstrating that Yki is required for melanotic tumors in this background. These results support a model in which elevated Yki signaling increases the number of hemocytes, which become melanotic tumors as a result of elevated JAK/STAT signaling. KEYWORDS Drosophila melanogaster hopscotch Stat92E hippo expanded yorkie bantam warts Myc The JAK/STAT pathway is evolutionarily conserved and plays critical roles in numerous developmental processes, including hematopoiesis (Levy 1999; Amoyel et al. 2014). JAKs are nonreceptor cytosolic tyrosine kinases that are normally activated by cytokines interacting with their cell surface receptors. The activated JAK-receptor complexes induce phosphorylation of STATs that subsequently bind specific DNA sequences and act as transcription factors (O'Shea et al. 2002). A dominant-active allele JAK2 V617F leads to a constitutively-active, ligandindependent protein (