Dependence of histamine release from rat mast cells on adenosine triphosphate (original) (raw)
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Naunyn-Schmiedeberg's Archives of Pharmacology, 1975
The ATP content of rat peritoneal mast cells has been studied in relation to histamine release induced by compound 48/80 and antigen-antibody (anaphylactic) reaction in vitro. When the ATP content of actively sensitized mast cells was reduced to different levels by oligomycin, a good correlation was obtained between the ATP levels and the amounts of histamine released by the anaphylaetic reaction.A similar linear relation has previously been demonstrated between the ATP levels of mast cells and histamine release induced by compound 48]80. The ATP content of mast cells was also studied at different intervals after the exposure of the cells to antigen or compound 48/80. No significant change in the ATP content was observed in untreated mast cells during the short period when histamine release occurs. If, however, the mast cells were preincubated with oligomycin or 2-deoxyglucose to reduce the rate of ATP synthesis while a large part of the histamine release remained tmaffected-a decrease in the ATP content could be demonstrated in close time relation to both anaphylactic and compound 48/80-induced histamine release. The observations indicate an increased utilization of ATP in mast cells during the release process.
British Journal of Pharmacology, 1979
The role of endogenous adenosine triphosphate (ATP) in histamine release from rat mast cells induced by the ionophore A23187 in vitro has been studied. 2 The amount of histamine released by calcium from rat mast cells primed with the ionophore A23187 was dependent on the ATP content of the mast cells. 3 In aerobic experiments a drastic reduction in mast cell ATP content was found during the time when histamine release induced by A23187 takes place. 4 Anaerobic experiments were performed with metabolic inhibitors (antimycin A, oligomycin, and carbonyl cyanide p-trifluorometroxyphenylhydrazone), which are known to block the energy-dependent calcium uptake by isolated mitochondria. The mast cell ATP content was reduced during A23187-induced histamine release under anaerobic conditions in the presence of glucose. This indicates an increased utilization of ATP during the release process. 5 The observations are consistent with the view that energy requiring processes are involved in ionophore-induced histamine release from rat mast cells although part of the ATP reduction in the aerobic experiments may be due to an uncoupling effect of calcium on the oxidative phosphorylation.
British Journal of Pharmacology, 1980
The relation between A23187-induced histamine release and the energy metabolism of the rat mast cells has been studied. 2 Ethacrynic acid was used as an inhibitor of calcium-induced histamine release from mast cells primed with the ionophore A23187, and to study calcium-induced changes in the adenosine triphosphate (ATP) content and the rate of lactate production of A23187-primed mast cells. 3 Ethacrynic acid by itself decreased the rate of glycolytic ATP production. 4 By measurement of the ATP content and the lactate production of mast cells with or without secretory activity, the increased demand of energy for exocytosis was estimated to be equivalent tQ 0.14 pmol of ATP per I03 mast cells.
FEBS Letters, 1990
Determination of the cellular content of adenosine triphosphate (ATP) and the rate of ATP-synthesis were used to estimate the cellular utilization of ATP in relation to anaphylactic histamine secretion. There was an increased rate of oxidative ATP-synthesis and a decreased cellular ATP content during the time period of histamine secretion and immediately after its completion. During secretion the additional ATP-utilization above the basal level of ATP-synthesis was 0.51 pmol/lp cells. 2.5 mm after cell activation, the rate of additional ATP-utilization was 0.30 pmol/l(Y cells/min, and the persistent ATP-decrease observed after 30 and 120 min may be due to a decreased rate of oxidative ATP-synthesis. Mast cell; Adenosine t~phosphate; Anaphylaxis; Histamine secretion; Exocytosis 2.3. Determination of histamine secretion and the cellular A TP content
Energy metabolism in rat mast cells in relation to histamine secretion
Pharmacology & Toxicology, 1987
The present survey is based on the following publications Johansen T, Chakravarty N. Dependence of histamine release from rat mast cells on adenosine triphosphate. Naunyn-Schmiedeberg's Arch Pharmacol 1972; 275: 457-63. Johansen T, Chakravarty N. The utilization of adenosine triphosphate in rat mast cells during histamine release induced by anaphylactic reaction and compound 48/80. Naunyn-Schmiedeberg's Arch Pharma-Johansen T. Adenosine triphosphate levels during anaphylactic histamine release in rat mast cells in vitro. Effects of glycolytic and respiratory inhibitors.
The mechanism of histamine release from mast cells
Biochemical Pharmacology, 1972
Mast cell secretion was studied in vitro using rat peritoneal cells stimulated by polymyxin B sulfate. The dose-response curve for histamine release was only slightly lowered when mast cells isolated by sedimentation through albumin solution (specific gravity, 1.100) were compared to cells not subjected to the isolation procedure. The release of N-acetyl+-glucosaminidase, a readily soluble component of mast cell granules, closely paralleled the release of histamine. However, little release occurred of two insoluble granule components, mast cell chymase and heparin, and their release was not dose dependent. These results indicated that histamine release from mast cells can occur in the absence of extrusion of their granules. Quantitative studies of the uptake of ruthenium red and morphologic studies of the distribution of ruthenium red and ferritin demonstrated that the granules are effectively extruded into an extracellular space tnat is confined to the cellular domain by a labyrinth of cytoplasmic processes. The secretory process of mast cells then appears to be effected through a sequence of membrane fusions that produce deep channels of extracellular space penetrating through the cell and enveloping the granules rather than by the propulsion of the granules to the cell surface with extrusion at that site.
Functional compartments in rat mast cells for cAMP and calcium on histamine release
Cellular Signalling, 2000
The crosstalk between 3′, 5′-cyclic adenosine monophosphate (cAMP), intracellular calcium, and histamine release in rat mast cells using the stimulatory effect of three different drugs, thapsigargin, sodium fluoride (NaF), and compound 48/80 were studied. Each of these drugs induces histamine release by different mechanisms. The transducting pathways modulating cAMP and intracellular calcium levels were modified by using, cholera toxin (CTX)
Cancer Chemotherapy and Pharmacology, 1992
We studied the histamine-releasing activity of several antineoplastic drugs on rat pleural and peritoneal mast cells. The drugs tested included the nitrogen mustards cyclophosphamide and ifosfamide, the nitrosourea carmustine, the triazene dacarbazine, the folic acid analogue methotrexate, the pyrimidine analogue cytarabine and fluorouracil, the vinca alkaloids vinblastine, vincristine and Vinoretbine, the epipodophyllotoxins etoposide and teniposide, and the enzyme L-asparaginase. Methotrexate, carmustine, fluorouracil, vinblastine and vincristine failed to elicit histamine release on rat mast cells. All of the other drugs evoked histamine release in both the presence and the absence of extracellular calcium, but ifosfamide, cytarabine and asparaginase induced a much lower release in the absence of this cation. The response elicited by cytarabine and etoposide was much higher in pleural than in peritoneal mast cells. These results indicate that some antineoplastic drugs may directly activate the release of histamine, which could contribute to some of their secondary effects.
Mast Cell Degranulation and Histamine Release Observed in a New in Vitro System
Journal of Experimental Medicine, 1960
Mast cells participate in some types of inflammatory reactions involving changes in the microcirculation of certain tissues. Among the known vasoactive substances of importance, histamine has been found in mast cells (1) of several species of animals and in certain species serotonin is also present (2). A variety of substances (the formaldehyde polymer of p-methoxyphenethylmethylamine (48/80), ovomucoid, and dextran) when administered to the rat elicit an inflammatory response, cause histamine and serotonin release and the morphological change of degranulation . No clear description of the process involved in release of the active amines and other mast cell constituents has yet been presented. A major obstacle has been the lack of an appropriate way of observing the action of various agents on the structure of the mast cell and its constituents under controlled conditions. Hence, a new method of observation was sought to study the mechanism of mast cell secretion in vitro.