Detection of human papilloma virus in potentially malignant and malignant lesions of the oral cavity and a study of associated risk factors (original) (raw)
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Human Papillomavirus (HPV) in Oral Cavity Lesions: Comparison with Other Oral Cancer Risk Factors
Journal of Microbiology Research, 2013
Hu man papillo mavirus (HPV) is considered a necessary factor for the development of cervical cancer; however, its relat ionship with oral cancer is controversial. The aim of this study was detect the presence of HPV in lesions of the oral cavity and its correlation with other risk factors. Presence of HPV was studied by polymerase chain reaction in samples fro m benign lesions, potentially malignant lesions (PML), neoplasias and healthy mucosae. The results from the different groups were co mpared; in addition to their histopathological variab les with tobacco smoking, alcohol consumption, so on. HPV was detected in 88.89% of benign lesions, 41.38% of PM L and 56.25% of neoplasias. The most prevalent genotypes were 16 and 6. Together, reached 55% of the total number of cases. A significant association was observed between HPV and male gender, tobacco smokers, alcohol drin kers and benign lesions. Tobacco smoking and alcohol intake were associated to neoplasias. Our results showed that factors like tobacco smoking and alcohol drinking, have more influence than HPV in the development o f oral neoplasias; however, 56.2% of the neoplasias tested positive for HPV; the percentage of HR-HPV detection increased with the severity of the lesions, suggesting its possible involvement in malignant processes
Role of human papilloma virus in oral squamous cell carcinoma: Review of literature
2017
Head and neck cancer is a major problem in our country constituting around one-third of all cancer cases in contrast to 4-5% in the developed world. The incidence of tobacco-related head and neck cancers in the western countries has declined due to decreased use of tobacco. Parallel to this decrease, there has been an increase in Human Papilloma virus (HPV) related oral cancers. In India also some attempts are made by the government to decrease the use of tobacco. However, the changing epidemiology seen there, is not seen here. We searched Pub Med database for literature published from 1985 to 2013 reporting any relationship between HPV and oral cancers to know why there is this difference in carcinogenic process.
JNCI Journal of the …, 2003
Background: Human papillomavirus (HPV), the causal agent of cervical cancer, appears to be involved in the etiology of cancer of the oral cavity and oropharynx. To investigate these associations, we conducted a multicenter case-control study of cancer of the oral cavity and oropharynx in nine countries. Methods: We recruited 1670 case patients (1415 with cancer of the oral cavity and 255 with cancer of the oropharynx) and 1732 control subjects and obtained an interview, oral exfoliated cells, and blood from all participants and fresh biopsy specimens from case patients. HPV DNA was detected by polymerase chain reaction (PCR). Antibodies against HPV16 L1, E6, and E7 proteins in plasma were detected with enzyme-linked immunosorbent assays. Multivariable models were used for case-control and casecase comparisons. Results: HPV DNA was detected in biopsy specimens of 3.9% (95% confidence interval [CI] ؍ 2.5% to 5.3%) of 766 cancers of the oral cavity with valid PCR results and 18.3% (95% CI ؍ 12.0% to 24.7%) of 142 cancers of the oropharynx (oropharynx and tonsil combined) with valid PCR results. HPV DNA in cancer biopsy specimens was detected less frequently among tobacco smokers and paan chewers and more frequently among subjects who reported more than one sexual partner or who practiced oral sex. HPV16 DNA was found in 94.7% of HPV DNA-positive case patients. HPV DNA in exfoliated cells was not associated with cancer risk or with HPV DNA detection in biopsy specimens. Antibodies against HPV16 L1 were associated with risk for cancers of the oral cavity (odds ratio [OR] ؍ 1.5, 95% CI ؍ 1.1 to 2.1) and the oropharynx (OR ؍ 3.5, 95% CI ؍ 2.1 to 5.9). Antibodies against HPV16 E6 or E7 were also associated with risk for cancers of the oral cavity (OR ؍ 2.9, 95% CI ؍ 1.7 to 4.8) and the oropharynx (OR ؍ 9.2, 95% CI ؍ 4.8 to 17.7). Conclusions: HPV appears to play an etiologic role in many cancers of the oropharynx and possibly a small subgroup of cancers of the oral cavity. The most common HPV type in genital cancers (HPV16) was also the most common in these tumors. The mechanism of transmission of HPV to the oral cavity warrants further investigation. [J Natl Cancer Inst 2003;95:
2011
We aimed to determine the role of HPV in the pathogenesis and outcome of oropharyngeal squamous cell carcinoma (OSCC) in lifelong nonsmoking and nondrinking patients. A case-case analysis was performed to compare the presence of HPV-DNA in tumor cells of 16 nonsmoking and nondrinking with 16 matched smoking and drinking patients (matching criteria: age at incidence, gender, tumor sublocation, tumor stage). HPV was detected using 2 PCR tests, FISH analysis, and p16(INK4A) immunostaining. Nonsmoking and nondrinking patients had more HPV-positive tumors than smoking and drinking patients (n = 12; 75% versus n = 2; 13%; P < 0.001). All HPV-positive tumors showed p16(INK4A) overexpression, and 1 HPV-negative tumor had p16(INK4A) overexpression, (P < 0.001). Overall survival and disease-specific survival were higher for HPV-positive compared to HPV-negative cases (P = 0.027, P = 0.039, resp.). In conclusion, HPV is strongly associated with OSCC of nonsmoking and nondrinking patients...
Human papilloma virus in oral squamous cell carcinoma in a Mexican population
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2005
Objective. To determine the human papilloma virus (HPV) infection in oral cancer and its association with smoking and drinking habits. Study design. A cross-sectional study was performed; samples were collected from 51 patients with histological diagnosis of squamous-cell carcinoma were collected at the Instituto Nacional de Cancerología in Mexico City. HPV infection was detected by polymerase chain reaction, and the clinical characteristics of this population were analyzed.
O195. The prevalence of human papillomavirus (HPV) in oral and oropharynx squamous cell carcinoma
Oral Oncology Supplement, 2009
The aim of this study was to analyse the prevalence of human papillomavirus (HPV) infection among patients with oral and oropharynx squamous cell carcinoma (OSCC). The correlation between HPV infection and OSCC, HPV genotypes, and correlation between HPV, OSCC, alcohol use, tobacco smoking, demographic data (gender, age, place of residence), anatomic location, pretreatment staging, metastases of lymph node evidence, and grading was investigated. In the examination group, there were 60 patients with squamous cell carcinoma (SCC), 54 males and six females. Twenty-one patients were affected with oral, 24-with oropharynx, and 15-with oral and oropharynx SCC. The patients were not subjected to chemotherapy and radiotherapy before operation. The examination samples were collected from paraffin sections. This analysis involved DNA isolation from histological specimens, an amplification of a fragment of human β-globin gene (reference gene), electrophoresis of amplification products of the gene, a quantitative DNA analysis (using spectrophotometric method), PCR, and genotyping. HPV DNA was detected in 25% of patients with SCC. Among HPV positive patients, 86.7% of the patients were infected with HPV type 16 and 13.3% were infected with another non identified HPV.
Dentino : Jurnal Kedokteran Gigi
ABSTRACTIntroduction: As many as 90%-95% cases of oral cancer are oral squamous cell carcinoma. In South and Southeast Asia, the prevalence of oral cancer is high. Oral cancer is ranked sixth for the most frequent malignancies in Asia with nearly 274,300 new cases occurring every year. Head and Neck Squamous Cell Carcinoma is one of the main public health concerns with 1-2% incident of all cancers worldwide and is also the sixth most common malignant tumor. Human Papillomavirus (HPV) is a DNA virus which is also known as the cause of head and neck cancer. Among all malignancies, oral cancer is one of the conditions where salivary examination can be used to detect oral cancer because it contacts directly with the lesions. The relationship between human papilloma virus (HPV) and oral squamous cell carcinoma has been extensively studied and shows that HPV-16 & 18 can be detected in saliva, serum or plasma samples and patient biopsies. Purpose: This systematic review aims to reveal the ...
Assessment of Role of Human Papilloma Virus in Patients with Oral Squamous Cell Carcinoma
Journal of College of Medical Sciences-Nepal
Background: Head and neck malignancies include malignancies instigating in the oral cavity, the orophar-ynx, the hypopharynx and the larynx. We planned the present study to assess the role of human papilloma virus (HPV) in patients with oral squamous cell carcinoma (OSCC). Methods: The present study included assessment of role of human papilloma virus in patients with oral squamous cell carcinoma. A total of 50 histopathologic proven cases of well differentiated OSCC were included in the present study. A total of 50 healthy oral mucosal tissues (controls) were also included in the present study. The controls were obtained while surgical removing the impacted teeth. DNA was obtained from the tissue sections and underwent Polymerization chain reaction for identification of HPV 16/18. All the results were summarized in Microsoft excel sheet and were analyzed by SPSS software. Results: HPV was absent in all the cases of control group, whereas among the OSCC group, the HPV was detected i...
2013
Background: Human papillomavirus (HPV), the causal agent of cervical cancer, appears to be involved in the etiology of cancer of the oral cavity and oropharynx. To investigate these associations, we conducted a multicenter case–control study of cancer of the oral cavity and oropharynx in nine countries. Methods: We recruited 1670 case patients (1415 with cancer of the oral cavity and 255 with cancer of the oropharynx) and 1732 control subjects and obtained an interview, oral exfoliated cells, and blood from all participants and fresh biopsy specimens from case patients. HPV DNA was detected by polymerase chain reaction (PCR). Antibodies against HPV16 L1, E6, and E7 proteins in plasma were detected with enzyme-linked immunosorbent assays. Multivariable models were used for case–control and case– case comparisons. Results: HPV DNA was detected in biopsy