A Different Approach to Assess Oxidative Stress in Dengue Hemorrhagic Fever Patients Through The Calculation of Oxidative Stress Index (original) (raw)
Related papers
Oxidative stress in adult dengue patients
The American journal of tropical medicine and hygiene, 2004
An association between viral diseases and increased oxidative stress has been suggested. The time course of serum levels of total antioxidant status (TAS), peroxidation potential (PP), glutathione (GSH), lipid peroxidation measured as hydroperoxides, and malondyaldehyde and 4-hydroxyalkenals (MDA + 4-HDA), as well as antioxidant enzymatic activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx), were measured in 22 serologically confirmed dengue patients. Most of the patients had dengue fever and three of them had dengue hemorrhagic fever. The redox parameters were compared with those of age- and sex- matched controls. No significant difference was observed for levels of GSH and TAS between patients and controls. Levels of PP, MDA + 4-HDA, and SOD were significantly higher. Levels of GPx and total hydroperoxides were significantly lower in patients in comparison with controls. These findings suggest that the alteration in redox status could result of increased oxidati...
Oxidative Stress Biomarkers as Prognostic Indicators of Severity in Patients With Dengue
Global Journal of Health Science
There is evidence for the role of oxidative stress in severe dengue pathogenesis. However, previous observational studies presents certain methodological limitations, which may affect its internal and external validity. This study was a case-control analysis of patients with severe dengue and dengue with warning signs, to evaluate the serum protein carbonyls-PCOs and lipid hydroperoxides-LOOHs levels and activities of superoxide dismutases-SODs (MnSOD, Cu/ZnSOD and total SOD), as potential prognosis indicators of severity in dengue patients, using binary logistic regression analysis and strategy of double cross-validation. Therefore, the study population was subdivided into a derivation group (pediatric patients, Barranquilla-Colombia) and an external validation group (children and adults patients, National Institute of Health of Peru). PCOs was the only oxidative stress markers that showed a strongest association with the severity of dengue, both in children and adults. In the deri...
Oxidative damage in dengue fever
Free Radical Biology and Medicine, 2009
Oxidative stress may be important in the pathogenesis of dengue infection. Using accurate markers of oxidative damage, we assessed the extent of oxidative damage in dengue patients. The levels of hydroxyeicosatetraenoic acid products (HETEs), F 2 -isoprostanes (F 2 -IsoPs), and cholesterol oxidation products (COPs) were measured in 28 adult dengue patients and 28 age-matched study controls during the febrile, defervescent, and convalescent stages of infection. We compared the absolute and the percentage change in these markers in relation to key clinical parameters and inflammatory markers. The levels of total HETEs and total HETEs/arachidonate, total F 2 -IsoPs/arachidonate, and COPs/cholesterol were higher during the febrile compared to the convalescent level. Total HETEs correlated positively with admission systolic blood pressure (r = 0.52, p b 0.05), whereas an inverse relationship was found between 7β-hydroxycholesterol and systolic and diastolic blood pressure (r = −0.61 and −0.59, respectively, p b 0.01). The urinary F 2 -IsoP level was higher in urine during the febrile stage compared to the convalescent level. Despite lower total cholesterol levels during the febrile stage compared to convalescent levels, a higher percentage of cholesterol was found as COPs (7β-, 24-, and 27-hydroxycholesterol). The levels of platelet-activating factoracetylhydrolase activity, vascular cellular adhesion molecule-1, tumor necrosis factor-α, and high-sensitivity C-reactive protein were higher during the febrile stage compared to their convalescent levels (p b 0.01). Markers of oxidative damage are altered during the various stages of dengue infection.
Acta Tropica, 2008
Objectives: Oxidative stress in dengue viral infection has been suggested and severity of it was found to be associated with progress of illness. Hence assessing oxidative stress mediated changes in plasma proteins can be an early biomarker for prediction of severe dengue infection. Design and methods: Thirty two dengue hemorrhagic fever (DHF), 21 dengue shock syndrome (DSS), 27 dengue fever (DF) and 63 age and sex matched controls, were included in this study. Blood samples were collected on the 3rd day of fever. Protein carbonylation (PCOs) and protein-bound sulphydryl (PBSH) group levels were determined by spectrophotometric method and analyzed as predictor of dengue hemorrhagic fever and dengue shock syndrome. Results: About 80-84% of cases presented with no signs of DHF/DSS at the time of sampling. Dengue infected individuals had significantly elevated PCOs and low PBSH group levels than the controls. Using one-way ANOVA we found a significant difference with high PCOs and low PBSH group levels between DHF and DSS when compared with DF (P < 0.001). However, no difference was observed in PBSH group levels between DHF and DSS. A significant difference in PCOs to PBSH ratio was observed among DF, DHF and DSS (P < 0.001). Linear regression analysis revealed that duration of hospitalization is dependent on PCOs and PBSH group levels. Receiver operator curve (ROC) analysis indicated that 5.22 nmol/mg protein PCOs; 1.08 PCOs to PBSH group levels ratio were optimal cutoff value for predicting DHF with sensitivity and specificity of 87.5% and 74.1%; 96.9% and 81.5%, respectively. For DSS prediction, 6.13 nmol/mg protein PCOs; 1.16 PCOs to PBSH group levels ratio were found as effective cutoff with sensitivity and specificity of 81% and 71.9%; 95.2% and 56.2%, respectively. Conclusion: Oxidative stress has been observed to develop since early days of onset of dengue infection. Plasma PCOs, PCOs to PBSH group ratio were found to very well predict DHF/DSS.
ANALYSIS OF ANTIOXIDANTS LEVEL IN DENGUE FEVER
Introduction: Dengue is the most frequent viral infection, with > 100 million infections throughout the world annually, including 250,000-500,000 cases of dengue hemorrhagic fever (DHF) and 24,000 deaths. Objectives of the study: The main objective of the study is to analyse the levels of antioxidants in dengue fever among local population of Pakistan. Material and methods: This cross-sectional study was conducted in Nishtar Hospital, Multan during June 2019 to December 2019. The data was collected from 100 patients of both genders. Antioxidants levels in the blood were measured by getting blood samples of patients. 5cc blood sample was taken and then it centrifuged at 4000rpm for the separation of serum. Results: The data was collected from 100 patients of both male and female patients. SOD activity in the serum and liver was dramatically and significantly decreased after infection when compared to normal values. MDA levels in the serum were significantly elevated when compared to the controls (p<0.05). CAT activity in the liver showed a significant decrease when compared with the controls (p<0.05), whereas there was no obvious change in CAT levels in the serum. Conclusion: It is concluded that dengue viral infection results in a significant alteration in the oxidative status in the liver, as shown by increased ROS production, MDA levels and the GSSG/GSH ratio and decreased levels of SOD and CAT.
Oxidative-nitrosative stress and dengue disease: a systematic review of in vivo/in vitro studies
Revista Cubana de Medicina Tropical, 2015
Objective : In this systematic review the aim was to summarise the in vivo / in vitro evidence on the role of oxidative-nitrosative stress in pathogenesis of dengue. Methods : We searched electronic databases (PubMed, EMBASE, The COCHRANE library, ScienceDirect, Scopus, SciELO, LILACS via Virtual Health Library, Google Scholar) using the term: dengue, dengue virus, severe dengue, oxidative stress, nitrosative stress, antioxidants, oxidants, free radicals, oxidized lipid products, lipid peroxides, nitric oxide, and nitric oxide synthase. Articles were selected for review by title and abstract excluding letter, review, epidemiological studies, and duplicates studies. Selected articles were reviewed for used animal model or cell cultures, original purposes, strain of virus or type of antibody, main outcomes, methods, and oxidative-nitrosative stress markers values. Results: In total, 4330 non-duplicates articles were identified from computerized searches of reference databases, of whic...
PLoS ONE, 2013
Changes in immune response during lifespan of man are well known. These changes involve decreased neonatal and elderly immune response. In addition, it has been shown a relationship between immune and oxidative mechanisms, suggesting that altered immune response could be associated to altered oxidative response. Increased expression of nitric oxide (NO) has been documented in dengue and in monocyte cultures infected with different types of dengue virus. However, there is no information about the age-dependent NO oxidative response in humans infected by dengue virus. In this study, monocyte cultures from neonatal, elderly and adult individuals (n = 10 each group) were infected with different dengue virus types (DENV-1 to 4) and oxidative/antioxidative responses and apoptosis were measured at days 1 and 3 of culture. Increased production of NO, lipid peroxidation and enzymatic and nonenzymatic anti-oxidative responses in dengue infected monocyte cultures were observed. However, neonatal and elderly monocytes had lower values of studied parameters when compared to those in adult-derived cultures. Apoptosis was present in infected monocytes with higher values at day 3 of culture. This reduced oxidant/antioxidant response of neonatal and elderly monocytes could be relevant in the pathogenesis of dengue disease.