Screening and Identification of Thermophilic Glycosyl Hydrolases Producing Microorganisms (original) (raw)

Journal of Biotechnology & Bioresearch

This study focuses on screening and identification of bacteria, which can produce alkaline xylanase at alkaline pH and high temperature. Bacterial isolates from corncob decaying soil, capable of hydrolyzing xylan were screened. Selected and purified 108 bacterial colonies grown on xylan-nutrient agar slants were activated and transferred into the fermentation medium. Six highest xylanase producing isolates were selected for further studies. Isolates CS 1 [132.0(±0.09)], CS 27 [121.3(±0.11)]& CS 88 [124.8(±0.44) UmL-1 ] showed highest xylanase production at pH 8.5 while isolates CS 52 [124.4(±0.01) UmL-1 ], CS 93 [113.0(±0.48) UmL-1 ] and CS 104 [110.1(±0.54) UmL-1 ] showed at pH 8.0 and 45 o C. Therefore isolates CS 1 , CS 27 & CS 88 were selected and the xylanase produced by them were screened for the kinetic properties. The crude enzymes of the isolates CS 1 , CS 27 & CS 88 showed zero order kinetics up to 4 min. The optimum temperature for the activity of the xylanase from isolates CS 1 and CS 27 was 55 o C, while that of isolate CS 88 was 60 o C. The optimum pH value for the xylanase from isolate CS 1 and CS 88 was 8.4 and that of isolate CS 27 was 8.0. Based on the kinetic properties of xylanase, isolates CS 1 and CS 88 were selected and characterized and found to be belonging to genus Bacillus. As Bacillus CS 1 produced highest xylanase activities, 16S rDNA was analyzed and identified as Bacillus pumilus and selected for further studies to produce xylanase at 45 o C and pH 8.5.

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