Armigeres subalbatus incriminated as a vector of zoonotic Brugia pahangi filariasis in suburban Kuala Lumpur, Peninsular Malaysia (original) (raw)
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Study on vector mosquito of zoonotic Brugia malayi in Musi Rawas, South Sumatera, Indonesia
Veterinary World, 2019
Background and Aim: Studies to determine abundance, distribution, species composition, and mosquito interactions are very important in understanding the risk of disease transmission to implement appropriate mosquito management in endemic areas. Lymphatic filarial worms are one of the parasites that are contracted and/or transmitted by mosquitoes when sucking the blood of infected humans or animals and then biting others. This research was conducted to study the abundance, species composition, mosquito biting cycles, density and periodicity of mosquitoes caught in Lubuk Pauh Village, Bulang Tengah Suku Ulu, Musi Rawas, South Sumatera, Indonesia, which is an endemic area of zoonotic Brugia malayi. Materials and Methods: The mosquito collection was done in July 2018 using the human landing collection method for 11 h from 18.00 pm to 5.00 am Western Indonesian Time. The catching of mosquitoes was done both indoors and outdoors, and mosquitoes were identified under a dissecting microscope using an identification key to confirm their species. Detection of B. malayi larvae in mosquitoes was confirmed by dissection and polymerase chain reaction methods. Results: The caught mosquitoes consisted of four species: Armigeres subalbatus, Culex quinquefasciatus, Culex vishnui, and Mansonia uniformis. Based on the Shannon–Wiener index, Lubuk Pauh Village has low mosquito species diversity (0.210). Ar. subalbatus was the dominant mosquito in Lubuk Pauh Village with dominance number 95.08, and it had the most frequent activity in each of periods of indoor and outdoor collection, with the highest density (man-hour density) at 18.00-19.00 (51.750). B. malayi infective stage larvae were not found in all mosquito species caught. Conclusion: Existence of Ar. subalbatus, Cx. quinquefasciatus, and Ma. uniformis in Lubuk Pauh Village which is an endemic area of B. malayi shows that the area is at risk of lymphatic filariasis transmission.
Zoonotic Brugia pahangi filariasis in a suburbia of Kuala Lumpur City, Malaysia
Five local Malaysian patients with clinical manifestations consistent with lymphatic filariasis were referred to our medical centre between 2003 and 2006. Although no microfilariae (mf) were detected in their nocturnal blood samples, all were diagnosed to have lymphatic filariasis on the basis of clinical findings and positive serology results. PCR on their blood samples revealed that two of the patients were infected with Brugia pahangi, an animal filarial worm hitherto not known to cause human disease in the natural environment. All the patients were successfully treated with anti-filarial drugs: four patients were treated with a combination of diethylcarbamazine (DEC) and albendazole, and one with doxycycline. Four of them were residents of Petaling Jaya, a residential suburbia located 10 km southwest of Kuala Lumpur city, Malaysia. The fifth patient was a frequent visitor of the suburbia. This suburbia has no history or record of B. malayi infection. The most likely vector of the worm was Armigeres subalbatus as extensive entomological surveys within the suburbia revealed only adult females of this mosquito species were infected with B. pahangi larvae. Wild monkeys caught in the suburbia were free from B. pahangi mf, but domestic cats were mf positive. This suggests that infected cats might be the source of the zoonotic infection in the suburbia. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
The American Journal of Tropical Medicine and Hygiene, 2007
Xenomonitoring (detection of filarial larvae or their DNA in mosquitoes) is a sensitive marker for assessing the endemicity of filariasis and a useful tool for evaluating elimination programs. To examine the fate of microfilariae (mf) and filarial DNA in vector competent and non-competent mosquito strains, we compared the detection of Brugia malayi parasites by dissection and by quantitative real-time polymerase chain reaction (PCR) in three different mosquito strains. We conclude that PCR is much more sensitive than dissection for detecting filarial larvae, especially their remnants in mosquitoes. However, parasite DNA can be detected in both vector and non-vector mosquitoes for two weeks or longer after they ingest mf-positive blood. Thus, although xenomonitoring with vector and non-vector mosquito species may be a sensitive method for indirectly detecting filarial parasites in human populations, positive test results for parasite DNA in mosquitoes do not necessarily prove that transmission is ongoing in the study area. MATERIALS AND METHODS Mosquito colonies. Aedes aegypti black-eyed Liverpool (AeL), Ae. aegypti Rockefeller (AeR), and Culex pipiens Iowa (Cu) used in this study were maintained at the University of Wisconsin-Madison, as previously described. 10,11 These three mosquito strains were selected for this study based on their vector competence for B. malayi. AeL is a competent laboratory vector of B. malayi in which worms complete development to infective stage larvae, and AeR and Cu are both refractory mosquito strains. In AeR, mf penetrate the midgut and enter thoracic muscle cells where development ceases. In contrast, B. malayi mf do not penetrate the midgut in Cu mosquitoes but die in the midgut lumen (Christensen BM, unpublished data). Laboratory animals were handled according to guidelines approved by the Animal Care Committee at the University of Wisconsin-Madison.
Proceedings of the 4th International Symposium on Health Research (ISHR 2019), 2020
Mansonia is one of the genera of mosquitoes that play a role in the transmission of filariasis in North Hulu Sungai Utara undertook mass treatment of entire endemic communities from 2006 to 2015 to eliminate the transmission of the disease. However, post validation surveillance activities are required to ensure the gains achieved are sustained. Entomological survey was carried out to assess the mosquito vectors of the disease and determined the presence of infection in these vectors, testing the hypothesis that transmission has already been interrupted in North Hulu Sungai District. The study was conducted in Pihaung Village which is an endemic filariasis village, which adult filarial mosquito vectors were collected by using double net-trapped human bait method with modification. Abundance, species dominance were analyzed and PCR-based to determine DNA filarial of suspected vector. Total of 942 mosquitoes from five genera Anopheles, Aedes, Armigeres, Culex and Mansonia were captured at both sites. Of which, Mansonia has the highest relative abundance 29.96%. In this study periode resulted Mansonia dives Man Hour Density and Man Biting Rate were 4.7 and 10.5 respectively. PCR examination were detected Brugia malayi DNA on Mansonia as suspected vector in Pihaung Village. This study conclude the existing of Brugia malayi DNA in this area highlighting potential risk for the re-emergence of LF transmission. This study recommends routine vector control and strengthen surveillance is required to continuously monitor the filarial suspected vector density.
Veterinary World, 2020
Background and Aim: Lymphatic filariasis (LF) is a lesser-known parasitic disease, which contributes to significant decreases in overall health. This study investigated the presence of Brugia malayi in mosquitoes collected in the South Borneo Province, Indonesia. Materials and Methods: Mosquitoes were collected through bare leg collection methods after sunset in several areas of the Hukai and Gulinggang villages in the Balangan District. The collected mosquitoes were identified based on morphological features and dissected to find microfilaria and then pooled through species for polymerase chain reaction (PCR) microfilaria detection. Results: A total of 837 female mosquitoes consisting of at least 14 species were selected; they were dissected, and no microfilariae were found. Mosquitoes were divided into 69 pools for PCR analysis. PCR revealed that 8.7% (6/69) of the pools were positive for B. malayi, including Mansonia annulifera (4 pools), Aedes albopictus (1 pool), and Culex tritaeniorhynchus (1 pool). Conclusion: These results suggested that mosquito dissection was not an optimum method for finding microfilaria. M. annulifera, C. tritaeniorhynchus, and A. albopictus mosquitoes might play an important role in the transmission of LF in the Balangan District. Information from this study could be used for the prevention of transmission or vector control programs in Indonesia.
Fauna associated with Malayan filariasis transmission in Banyuasin, South Sumatra, Indonesia
Veterinary World, 2021
Background and Aim: Brugia malayi is known to be zoonotically important because it can be transmitted from animals (mammals and primates) to humans or from humans to humans through mosquito vectors. This study was conducted to explore the fauna associated with Malayan filariasis transmission in Sedang village, Suak Tapeh District, Banyuasin Regency, South Sumatra Province, Indonesia. Materials and Methods: A cross-sectional research design with an observational and analytical approach was applied in this study, and it was conducted in May 2018. Mosquitoes were collected twice using human bait both inside and outside the house from 6:00 p.m. to 6:00 a.m. The presence of competitors, predators, and reservoir hosts in the areas of five breeding habitats of Mansonia spp. was observed. The presence of microfilaria was confirmed under a microscope in night blood samples of inhabitants and cats. The presence of infective larvae (L3) of B. malayi was identified microscopically and based on the polymerase chain reaction method in female Mansonia mosquitoes. Results: A total of 12 mosquito species were found, among which Mansonia uniformis was the dominant mosquito, and the predominant competitor was Mansonia annulifera. Dragonflies, as predators were found in two breeding habitats and fish were found in one breeding habitat. The L3 of B. malayi were not identified in the mosquitoes, and the microfilariae of B. malayi were not found in the blood samples of inhabitants and cats. Conclusion: Although Mansonia mosquito population was abundant in Banyuasin Regency, the mosquito was not confirmed as an intermediate host of B. malayi, and the cat was not confirmed as a reservoir of B. malayi in the location.
Identification of some common infective filarial larvae in Malaysia
Journal of Helminthology, 1982
ABSTRACTInfective larvae of Wuchereria, Brugia, Breinlia, Dirofilaria and Setaria species from an experimental vector, Aedes togoi, are compared. The distinctive bubble-like caudal papillae of Wuchereria bancrofti are readily distinguishable from the protuberant ones of Brugia spp; the ‘ear-like’ papillae of Breinlia are distinct from the ‘knob-like’ ones of Dirofilaria or the ‘thorn-like’ terminal papilla of Setaria.
Detection of Brugia malayi in mosquitoes by the polymerase chain reaction
Journal of the American Mosquito Control Association, 1998
Accurate identification of filarial parasites in mosquitoes poses a major problem for the coordination of filariasis control programs. Traditional methods are tedious, and some are not specific enough to give satisfactory results. Amplification of specific gene sequences by primer-directed polymerase chain reaction (PCR) has been increasingly utilized as a diagnostic tool. However, current protocols for the extraction of parasite DNA from mosquito samples are tedious and could lead to failure of PCR amplification. We demonstrate that the use of Chelex is an efficient method for DNA extraction from mosquitoes and the parasite and that PCR amplification with primers specific for Brugia malayi yields a band of the expected size. The PCR products were transferred to a nylon membrane with Southern blotting, and a B. malayi-specific digoxigenin-labeled probe confirmed the sequence similarity of the PCR-amplified fragment and increased the sensitivity of the PCR assay. Use of this probe en...
Veterinary World, 2024
Background and Aim: Lymphatic filariasis (LF) is a vector-borne disease in various regions of Indonesia. The transmission dynamics within a locality are intricately linked to the presence of the pathogen (microfilaria), definitive host (humans), intermediate host (mosquitoes), reservoir, and environmental factors. The geographic landscape of Central Bengkulu Regency, which is characterized by plantations, marshlands, and forests, serves as a suitable habitat for mosquitoes. Understanding species diversity, vector behaviors, habitat characteristics, and microfilarial presence is crucial for devising effective and efficient control strategies. This study aimed to identify species diversity, assess biting patterns, characterize larval habitats, and detect microfilarial presence in mosquitoes. Materials and Methods: Mosquito collection was conducted using human landing collection (HLC) and resting collection indoors and outdoors for 6 months at a frequency of twice monthly from November 2022 to May 2023. The larvae were collected using dippers and pipettes. Adult mosquitoes and larvae were identified at the species level and analyzed using diversity indices. The measured larval bioecological parameters included physical, chemical, and biological conditions. The mosquito density obtained through HLC was calculated using the man-hour density (MHD) and man-biting rate (MBR) formulas. The presence of microfilaria was confirmed using a polymerase chain reaction. Results: A total of 808 adult mosquitoes from five genera and 18 species were captured, along with 485 larvae from four genera and eight species. The mosquito diversity was moderate. The dominant adult species included Armigeres subalbatus (44.8%), whereas Aedes albopictus (25.4%) and Ar. subalbatus (22.3%) were abundant larvae. The highest larval density was observed in natural ponds. The average MBR was three mosquitoes per person per night, with fluctuating nightly activity (mean MHD of 1.8 mosquitoes per person per hour). Larval habitats had temperatures of 25.4°C–28.7°C, illumination of 224–674 lx, and pH of 7.1–7.9, with over half being turbid and nearly two-thirds lacking predators. Microfilariae were not detected in the tested mosquitoes. Conclusion: The presence of mosquitoes, their habitat, and the high density of Ar. subalbatus contributes to the transmission of LF in Central Bengkulu Regency, Indonesia. Keywords: Bengkulu, characteristics, diversity, filariasis, habitat, vector.
E3S Web of Conferences
According to data from the Ministry of Health of the Republic of Indonesia in 2018, there were 37 cases of Elephantiasis in Yogyakarta, even though Yogyakarta is not an endemic area of filariasis. This preliminary study was carried out to evaluate the diversity of species, relative abundance, frequency and dominance of mosquitoes in Yogyakarta and to determine the role of mosquitoes as a vector of filariasis. A cross-sectional study was used involving 3 hamlets with elephantiasis cases found in April 2019 in Yogyakarta. Mosquitoes around the house inhabited by the patient with elephantiasis were collected for 12 hours starting from 6:00 p.m. until 6:00 a.m. Mosquitoes were dissected under a dissection microscope and the presence of filaria larvae was observed. A total of 9 mosquitoes species were found. They were Culex vishnui, Culex tritaenorhynchus, Armigeres kesseli, Aedes albopictus, Culex quinquefasciatus, Culex bitaenorhynchus, Anopheles vagus, Anopheles subpictus, Aedes aegyp...