Label-Free Nanometer-Resolution Imaging of Biological Architectures through Surface Enhanced Raman Scattering (original) (raw)

Label-free imaging of biological specimens can be enhanced through the coupling of Raman scattering with super-resolution techniques. This study demonstrates the potential of label-free super-resolution Raman imaging using stochastic reconstruction of temporal fluctuations in the surface enhanced Raman scattering (SERS) signals originating from biomolecular layers on engineered plasmonic surfaces that exhibit high hot-spot density. Achieving a resolution of 20 nm, the approach offers significant insights into biomolecular structures, showing blinking behaviors of the SERS signal that are influenced by excitation intensity. Overall, the findings present a novel method for chemically resolved imaging at the nanoscale in biological systems.