Mucosal tissue transglutaminase expression in celiac disease (original) (raw)
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Background/aims: Our aim in the present study was to investigate tissue transglutaminase expression by immunohisto-chemistry in duodenal mucosa of patients with celiac disease. Methods: A total of twelve patients with celiac disease were examined. The patients had different clinical and histopatho-logic degrees of severity and responded to gluten withdrawal with clinical improvement. Ten subjects with various unrelated diseases served as controls. Serum endomysium antibodies were measured by an indirect immunofluorescence method using a commercial kit. Duodenal biopsy specimens were stained with hematoxylin and eosin. Immunohistochemical staining for anti-tissue transglutaminase antibodies was performed using commercial kit. Results: Serum endomysium antibodies and evaluation of small bowel biopsy specimens were normal in all control subjects. However, serum endomysium antibodies were positive in all of the celiac patients. Immunohistochemical staining pattern of duodenal biopsy specimen performed using anti-tissue transglutaminase anti-bodies was similar in celiac patients and control subjects. Conclusion: Tissue transglutaminase expression by immuno-histochemical methods in untreated celiac mucosa is not suitable for diagnosis of celiac disease.
Tissue transglutaminase—the key player in celiac disease: a review
Autoimmunity Reviews, 2004
Gluten-sensitive enteropathy, otherwise known as celiac sprue, is characterized by an abnormal proximal small intestinal mucosa arising as a result of an inappropriate inflammatory response to ingested gluten antigens present in wheat in genetically susceptible individuals. This immune response is directed to a 33-mer peptide of the a gliadin component of gluten. The generation of an epitope for the recognition by CD4 T cells requires q deamination of the protein by tissue transglutaminase (tTG). Moreover, IgA anti tTG is highly sensitive and is specific serologic marker (95-99%) of celiac disease. They can be easily determined quantitatively, by ELISA of an accurate and relatively inexpensive technique. Therefore, tTG can be used as the first line diagnostic test in the work-up of celiac disease, as well as for screening purposes. Finally, tTG may contribute to future strategies in treating celiac disease either by producing nontoxic wheat or by generating oral vaccination that can prevent the disease. ᮊ
Gastroenterology Research and Practice, 2016
Celiac disease is usually diagnosed by demonstrating gluten enteropathy in small bowel biopsy. Celiac specific antibodies are used as an initial screening test. The goal of this study is to test the relationship of the anti-tTG titer and severity of histological changes in Jordanian children with celiac disease. Method. The medical records of 81 children who had elevated anti-tTG titer and had duodenal biopsies available were retrospectively reviewed. Result. Assessing the association of anti-tTG titer with duodenal histopathological changes, 94% of those with high anti-tTG titer (≥180 U/mL) had histological evidence of celiac disease. There was statistically significant positive association between high anti-tTG titer and Marsh grading as 82% of patients with Marsh III had high anti-tTG titer (Chi2 18.5; P value 0.00; Odds Ratio 8.5). The fraction of patients with Marsh III who were correctly identified as positive by anti-tTG titer ≥ 180 U/mL was high (sensitivity = 81.6). Moreove...
Annals of Pathology and Laboratory Medicine, 2019
A definite diagnosis of Celiac disease is based on histological changes, including intraepithelial lymphocytosis, crypt hyperplasia, and varying degrees of villous atrophy, graded according to a classification system proposed by Marsh in 1992 and now widely used as modified Marsh grading depicted in table 1. [6-9] Duodenal biopsy remains the gold standard for diagnosis of CD, even though there are very specific serologic tests like anti-gluten and anti-tissue transglutaminase (anti-tTG) antibodies. Correlation of clinical, serologic, and histological features is essential for the definitive diagnosis of this condition. [10]
Journal of clinical laboratory analysis, 2017
Anti-tissue transglutaminase (anti-tTG) and endomysium antibodies (EMA) are detectable in duodenal culture media of celiac disease (CD) patients. To improve the management of this organ culture system, we evaluated the anti-tTG occurrence by immunochromatographic assay (ICA). A total of 103 CD patients and 41 disease controls underwent duodenal biopsy for the organ culture. In culture supernatants, IgA anti-tTG were tested by both enzyme-linked immunosorbent assay (ELISA) and ICA, IgA EMA were searched by indirect immunofluorescence analysis (iIFA). Endomysium antibodies and anti-tTG measured by ELISA were positive in culture media of all CD patients, while anti-tTG detected by ICA were positive in culture media of 87/103 CD patients. Anti-tTG ICA scores significantly correlated with anti-tTG ELISA values (r=.71, P<.0001). Sensitivity, specificity and diagnostic accuracy of anti-tTG detected by ICA were 84.5%, 100% and 88.9%, respectively. Using ICA, anti-tTG are detectable in du...