Prognostic significance of Ki-67 (MIB1), PCNA and p53 in cancer of the oropharynx and oral cavity (original) (raw)
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Asian Journal of Medical Sciences
Background: Prognosis of oral squamous cell carcinoma (OSCC) can be predicted by evaluating both clinicopathological parameters and molecular markers. Understanding the reasons of recurrence is important to increase the morbidity and mortality of the patient. Objectives: The objectives are to study the immunoexpression of p53 and Ki-67 in recurrent and non recurrent OSCC. And correlate the clinicopathological parameters among non-recurrent and recurrent OSCC to predict the prognosis of OSCC. Methods: Clinicopathological and immunoexpression of p53 and Ki-67 at the histologically negative margin (HNM) and invasive tumor front (ITF) were assessed in 50 cases of non-recurrent and recurrent OSCC. Overall survival and disease free survival was estimated. Statistical tests like Mann Whitney U test, t Test, Pearson correlation coefficient, Kaplan Meier curve and Log Rank test were used. Results: The Labelling Index (LI) of p53 and Ki-67 was marginally higher in recurrent than in non-recurr...
The Laryngoscope, 2001
Hypothesis: P53 and Ki-67 status will predict response to treatment, organ preservation, and survival in patients with advanced squamous cell cancers of the head and neck treated with chemoradiotherapy (CRT). Study Design: Retrospective analysis of p53 and Ki-67 status from the CRT arm of a randomized, controlled trial (n ؍ 50) and from patients receiving the same treatment but not enrolled in the trial (n ؍ 55). Methods: P53 and Ki-67 status were established from archived tissue samples using immunohistochemical (IHC) staining. Tumors were positive for p53 (p53؉) when more than 2% of cells stained for p53 and were positive for Ki-67 (Ki-67؉) when any cell stained for Ki-67. End points were tumor response, tumor recurrence, survival status, and organ preservation at last follow-up, and time to events. Predictive models were calculated for each outcome. Results: Neither marker predicted tumor response to treatment. P53؉ status was associated with tumor recurrence (P ؍ .003) and locoregional recurrence (P ؍ .003). Adjusting for time to event, p53؉ status was significantly related to a lower recurrence-free survival (P ؍ .004), lower disease-specific survival (P ؍ .04), lower overall survival with primary site preservation (P ؍ .03), and lower disease-specific survival with primary site preservation (P ؍ .003). Multivariate analysis revealed that p53؉ status was significantly related to a lower recurrence-free survival (P ؍ .01, risk ratio [RR] ؍ 3.65) and lower disease-specific survival with organ preservation (P ؍ .02, RR ؍ 3.41). Ki-67؉ status was not related to any variables. However, multivariate analysis revealed that Ki-67؉ was significantly related to a lower overall survival (P ؍ .05, RR ؍ 2.03). The combination of both markers negative (p53-/Ki-67-) was associated with a lower incidence of tumor recurrence (P ؍ .02), lower locoregional recurrence (P ؍ .01), and fewer second primary lesions (P ؍ .04). Adjusting for time to event, p53-/Ki-67-status was significantly related to a higher recurrence-free survival (P ؍ .02), higher disease-specific survival with primary site preservation (P ؍ .02), and higher overall survival with primary site preservation (P ؍ .02). Multivariate analysis revealed that p53-/Ki-67-status was significantly related to a higher overall survival with site preservation (P ؍ .01, RR ؍ 2.78). Conclusions: P53 and Ki-67 status appear to be related to the various survival end points considered in this study. However, this relation does not seem to be sufficient to warrant treatment modifications. Closer follow-up may be justified in both p53؉ and Ki67؉ patients to detect recurrence or a second primary at an earlier stage, possibly improving survival.
PCNA, Ki-67, p53, bcl-2 and prognosis in intraoral squamous cell carcinoma of the head and neck
Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology, 1997
Eighty patients with primary intraoral squamous cell carcinomas of the head and neck, with a follow-up of 4-14 years were analysed for clinical outcome in relation to immunohistochemical expression of PCNA, Ki-67, p53, bcl-2 and presence of mutations in the p53 gene. The tumor site was not associated with the different parameters calculated. PCNA and Ki-67 labelling showed median values of 56% and 32%, respectively, and neither antigen was of predictive value. Fifty-five percent of the tumours expressed p53, and 38 (48%) had mutations in the p53 gene. No association between the presence of p53 protein or mutations in the p53 gene and clinical outcome was found. Bcl-2 positivity was detected in a minor fraction (10%) of the tumours.
Second Primary Tumor: P53 and Ki-67 Expression in Patients with Oral Squamous Cell Carcinoma
Recent technical and scientific advances on oncology allowed increase of patients’ survival, sequelae reduction and prevention of other tumours, including second primary tumours (SPT). Indeed, some individuals with head and neck cancer manage to survive the first primary tumour, but cannot resist the second. For these reasons, studies to evaluate the factors related to SPT development of are greatly heightened. The aim of this study was correlate the immunoexpression of ki-67 and p53 proteins in oral squamous cell carcinoma (SCC) in two groups: Group A patients who suffered from a primary tumour of the mouth, and Group B patients who developed a SPT. All subjects studied had histological diagnoses of SCC and were admitted to the Erasto Gaertner Hospital, Brazil, from 1990 to 2005 – 35 at Group A and 20 at Group B. All patients’ medical records were reviewed, and immunohistochemistry reactions were realized for ki-67 and p53 proteins. Analysis was made using the chi-square test and t...
British journal of cancer, 1999
A study was made of the relationship between measurements of radiosensitivity versus proliferation and p53 status in head and neck cancers. Inherent tumour radiosensitivity was assessed as surviving fraction at 2 Gy (SF2) using a clonogenic soft agar assay (n = 77). The results were compared to data on proliferation obtained by both flow cytometry (labelling index (LI), the potential doubling time (Tpot) n = 55) and immunohistochemistry (Ki-67 and PCNA; n = 68), together with immunohistochemical p53 expression (n = 68). There were no overall significant differences in the median values of the various parameters analysed for the different sites within the head and neck region, disease stages, grades of tumour differentiation or nodal states. A subgroup analysis showed that oropharyngeal (n = 22) versus oral cavity (n = 35) tumours were more radiosensitive (P = 0.056) and had a higher Ki-67 index (P = 0.001). Node-positive tumours had higher LI (P = 0.021) and a trend towards lower Tp...
2010
OBJECTIVE: This study is aimed to evaluate the immunostaining influence of p53 and Ki67 proteins in areas of field cancerization of head and neck squamous cell carcinoma (HNSCC). We analyzed associations of these proteins with clinicopathological parameters and the relation between their immunoexpression in HNSCC. MATERIAL AND METHOD: In a retrospecive analysis, 40 patients with HNSCC were selected according to the recurrence of the disease, forming two groups: recurrent and non-recurrent HNSCC. Morphological gradations and imunnohistochemical analysis of p53 and Ki67 were performed in invasive front and tumor adjacent epithelium. RESULTS: It was found significant associations between tumor recurrence and p53 positivity in mucosa and invasive front. However, no association was found between p53 immunostaining and the clinicopathological parameters. Ki67 was not related to any clinicopathological parameter either. The association between Ki67 and p53 expression was not significant. T...
Head & Neck, 2004
Background. Aberrations of the p53 gene and overexpression of its protein are widely recognized markers of malignancy including oral squamous cell carcinomas. This study was performed to evaluate the relationship of immunoexpression of p53 protein in series of 91 squamous cell carcinomas of the oral cavity with clinicopathologic parameters and to investigate whether p53 immunoexpression might influence the clinical outcome of the disease.
Ki-67 Proliferation Rate as a Prognostic Marker in Major Salivary Gland Carcinomas
Annals of Otology, Rhinology & Laryngology, 2010
Objectives The study was performed to evaluate the prognostic relevance of cell proliferation associated with Ki-67/Mib-1 immunostaining in malignant tumors of the major salivary glands. Methods Cell proliferation was evaluated by Mib-1 antibody against Ki-67 antigen in 41 patients with cancer of the parotid or submandibular glands, including 14 acinic cell carcinomas, 12 ductal carcinomas, 7 mucoepidermoid carcinomas, 5 carcinomas ex pleomorphic adenoma, 1 adenoid cystic carcinoma, 1 undifferentiated carcinoma, and 1 polymorphous low-grade adenocarcinoma. Results Patients with Ki-67 values of more than 15% and those with Ki-67 values of 15% or less differed both in disease-free survival (p < 0.001) and in overall survival (p < 0.001). We evaluated the association between Ki-67 and time to recurrence in correlation to age, sex, ductal histotype, and N stage; the Cox regression model was significant (p = 0.013). In the group of patients with T1 and T2 cancers, those with Ki-67 ...