Repression of the heavy ferritin chain increases the labile iron pool of human K562 cells (original) (raw)

The role of ferritin in the modulation of the labile iron pool was examined by repressing the heavy subunit of ferritin in K562 cells transfected with an antisense construct. Repression of the heavy ferritin subunit evoked an increase in the chemical levels and prooxidant activity of the labile iron pool and, in turn, caused a Abbreviations used : H-FT, heavy ferritin subunit ; L-FT, light ferritin subunit ; LIP, labile iron pool ; ROS, reactive oxygen species ; TfR, transferrin receptor ; IRP, iron-responsive protein ; RT, reverse transcriptase ; DAPI, 4,6-diamidino-2-phenylindole ; carboxy-H 2 DCF, carboxydichlorodihydrofluorescin ; H 2 DCFDA-diAM, 2h-7h-carboxydichlorodihydrofluorescein-diacetate diacetoxymethyl ester. 1 To whom correspondence should be addressed (e-mail ioav!cc.huji.ac.il).