Age and sex influence on oxidative damage and functional status in human skeletal muscle (original) (raw)
Related papers
Oxidative stress" and muscle aging: influence of age, sex, fiber composition and function
2003
Aim of this research was to study human muscle aging and the influence of oxidative stress correlated with physiological factors (age, sex, fiber composition and function) by measuring the antioxidant enzymes activities: total and mitochondrial superoxide dismutase (total and MnSOD), catalase (CAT), glutathione peroxidase(GSHPx) the levels of glutathione and glutathione disulfide (GSH and GSSG) and redox index. We also measured the lipid peroxide amount . As about age-related changes, we studied 120 samples (18-91 year-old) and we noted the presence of a correlation between age and ROS-mediated damages. Futhermore, it seems that 65 years could be the age at which ROS-dependent damage becomes crucial and begins to show up. Our data about sex-dependent changes showed how males may be potentially more vulnerable to oxidative damage than females. Study about fiber composition in old group (65-90 year-old) reported that subjects with +40% type II fibers not only have lipoperoxide levels ...
Doria et al. Review - Human aging muscle and oxidative system pathway
Ageing is a complex process that in muscle is usually associated with a decrease in mass, strength, and velocity of contraction. One of the most striking effects of ageing on muscle is known as sarcopenia. This inevitable biological process is characterized by a general decline in the physiological and biochemical functions of the major systems. At the cellular level, aging is caused by a progressive decline in mitochondrial function that results in the accumulation of reactive oxygen species (ROS) generated by the addition of a single electron to the oxygen molecule. The aging process is characterized by an imbalance between an increase in the production of reactive oxygen species in the organism and the antioxidant defences as a whole. The goal of this review is to examine the results of existing studies on oxidative stress in aging human skeletal muscles, taking into account different physiological factors (sex, fibre composition, muscle type, and function).
Relationship between Human Aging Muscle and Oxidative System Pathway
Ageing is a complex process that in muscle is usually associated with a decrease in mass, strength, and velocity of contraction. One of the most striking effects of ageing on muscle is known as sarcopenia. This inevitable biological process is characterized by a general decline in the physiological and biochemical functions of the major systems. At the cellular level, aging is caused by a progressive decline in mitochondrial function that results in the accumulation of reactive oxygen species (ROS) generated by the addition of a single electron to the oxygen molecule. The aging process is characterized by an imbalance between an increase in the production of reactive oxygen species in the organism and the antioxidant defences as a whole. The goal of this review is to examine the results of existing studies on oxidative stress in aging human skeletal muscles, taking into account different physiological factors (sex, fibre composition, muscle type, and function).
Free Radical Biology and Medicine, 1999
This study was conducted in order to provide evidence for the role of reactive oxygen species (ROS) in human skeletal muscle aging. We used human muscle samples obtained from hospitalized patients in an open study with matched pairs of individuals of different ages. The subjects, ranging in age from 17 to 91 years, were grouped as follows: 17-25-, 26 -35-, 36 -45-, 46 -55-, 56 -65-, 66 -75-, 76 -85-, and 86 -91-year-old groups. To investigate the relationship between muscle aging and oxidative damage we measured total and Mn-dependent superoxide dismutase (total SOD, MnSOD), glutathione peroxidase (GSHPx), and catalase (CAT) activities; total reduced and oxidized glutathione (GSHtot, GSH, and GSSG) levels; lipid peroxidation (LPO), and protein carbonyl content (PrC). Total SOD activity decreases significantly with age in the 66 -75-year-old group, although MnSOD activity increases significantly in the 76 -85-year-old group. The activity of the two H 2 O 2 detoxifying enzymes (GSHPx and CAT) did not change with age, as do GSHtot and GSH levels. GSSG levels increased significantly (76 -85-and 86 -91-year-old groups) with age. We observed a significant increase in LPO levels (66 -75-and 76 -85-year-old groups), although the PrC content shows a trend of increase without gaining the statistical significance. These results support the idea that ROS play an important role in the human muscle aging process.
Reduced oxidative power but unchanged antioxidative capacity in skeletal muscle from aged humans
Pflügers Archiv : European journal of physiology, 2003
The hypothesis that the aging process is associated with mitochondrial dysfunction and oxidative stress has been investigated in human skeletal muscle. Muscle biopsy samples were taken from seven old male subjects [OS; 75 (range 61-86) years] and eight young male subjects [YS; 25 (22-31) years]. Oxidative function was measured both in permeabilised muscle fibres and isolated mitochondria. Despite matching the degree of physical activity, OS had a lower training status than YS as judged from pulmonary maximal O(2) consumption ( Vdot;O(2)max, -36%) and handgrip strength (-20%). Both maximal respiration and creatine-stimulated respiration were reduced in muscle fibres from OS (-32 and -34%, respectively). In contrast, respiration in isolated mitochondria was similar in OS and YS. The discrepancy might be explained by a biased harvest of "healthy" mitochondria and/or disruption of structural components during the process of isolation. Cytochrome C oxidase was reduced (-40%, P&...
Age-dependent increases in oxidative damage to DNA, lipids, and proteins in human skeletal muscle
Free Radical Biology and Medicine, 1999
A role for oxidative damage in normal aging is supported by studies in experimental animals, but there is limited evidence in man. We examined markers of oxidative damage to DNA, lipids, and proteins in 66 muscle biopsy specimens from humans aged 25 to 93 years. There were age-dependent increases in 8-hydroxy-2-deoxyguanosine (OH 8 dG), a marker of oxidative damage to DNA, in malondialdehyde (MDA), a marker of lipid peroxidation, and to a lesser extent in protein carbonyl groups, a marker of protein oxidation. The increases in OH 8 dG were significantly correlated with increases in MDA. These results provide evidence for a role of oxidative damage in human aging which may contribute to age-dependent losses of muscle strength and stamina.
Ageing: effects on oxidative function of skeletal muscle in vivo
Molecular and cellular biochemistry, 1997
31P magnetic resonance spectroscopy studies were carried out on calf muscle of 144 normal male and female subjects age 20-83 years in order to investigate age-related changes in muscle metabolism. Compared to the young adults (20-29 years), oxidative capacity was higher in the children (6-12 years) and was significantly decreased in the elderly (70-83 years). In the adults, the intracellular pH change during exercise diminished with increasing age, resulting in higher calculated free [ADP] and possibly serving as an adaptive mechanism to stimulate mitochondrial ATP production. Children also had higher pH and [ADP] in exercise, but unlike results from the elderly, this was associated with higher oxidative capacity and more rapid metabolic recovery from exercise.
Oxidative system in aged skeletal muscle
Muscles, ligaments and tendons journal, 2011
Aging is an inevitable biological process that is characterized by a general decline in the physiological and biochemical functions of the major systems. In the case of the neuromuscular system, reductions in strength and mobility cause a deterioration in motor performance, impaired mobility and disability. At the cellular level, aging is caused by a progressive decline in mitochondrial function that results in the accumulation of reactive oxygen species (ROS). As the level of oxidative stress in skeletal muscle increases with age, the age-process is characterized by an imbalance between an increase in ROS production in the organism, and antioxidant defences as a whole. We have reviewed the literature on oxidative stress in aging human skeletal muscles, and to assesss the impact of differences in physiological factors (sex, fiber composition, muscle type and function).
Free Radicals and Human Aging Muscle
Systems Biology of Free Radicals and Antioxidants, 2014
Aging is an inevitable biological process, characterized by a general decline in physiological and biochemical functions of the major systems. In the case of the neuromuscular system, reductions in strength and mobility cause a deterioration in motor performance, impaired mobility, and disability. At the cellular level, aging is caused by a progressive decline in mitochondrial function that results in accumulation of reactive oxygen species (ROS). As the level of oxidative stress in skeletal muscle increases with age, age process is characterized by an imbalance between an increase in ROS production in the organism and antioxidant defenses as a whole. The goal of this chapter is to examine the results of existing studies on oxidative stress in aging human skeletal muscles, taking into account (I) different physiological factors, such as sex, fiber composition, muscle type, and function; (II) biochemical alterations in muscle induced by ROS; and (III) oxidative stress markers related to aged muscle, particularly 8-hydroxy-2′-deoxyguanosine (8-OHdG), malondialdehyde (MDA), and levels of carbonyl residues such as in Carbonyl Proteins (PC). Finally, we analyze data, present in literature, regarding the beneficial effects of nutrition and physical activity in preventing oxidative damages associated with sarcopenia.