Characterization of microsatellite loci in the Jamaican fruit���eating bat Artibeus jamaicensis and cross���species amplification (original) (raw)
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Male dominance, paternity, and relatedness in the Jamaican fruit-eating bat (Artibeus jamaicensis)
Molecular Ecology, 2003
We analysed variation at 14 nuclear microsatellite loci to assess the genetic structure, relatedness, and paternity of polygynous Jamaican fruit-eating bats. A total of 84 adults captured in two caves exhibited little genetic differentiation between caves ( F ST = 0.008). Average relatedness among adult females in 10 harem groups was very low ( R = 0.014 ± ± ± ± 0.011), providing no evidence of harem structure. Dominant and subordinate males shared paternity in large groups, while dominant and satellite males shared paternity in smaller groups. However, our results suggest that male rank influences paternity. Dominant males fathered 69% of 40 offspring, followed by satellite (22%) and subordinate males (9%). Overall adult male bats are not closely related, however, in large harem groups we found that subordinate and dominant males exhibited relatedness values consistent with a father-offspring relationship. Because dominant and subordinate males also sired all the pups in large groups, we propose that their association provides inclusive fitness to them.
Conservation Genetics, 2008
Dendrocincla turdina has a poor ability to survive in fragmented areas and inhabits the depleted Brazilian Atlantic Forest. For the assessment of genetic diversity in this species, nineteen microsatellite loci were isolated and characterized, 11 of which were polymorphic for the population studied. Observed and expected heterozigosity ranged from 0.07 to 0.80 and 0.08 to 0.91, respectively. Cross-amplification was successfully obtained with two other species from the family Dendrocolaptidae: Xiphorhynchus fuscus and Sittasomus griseicapillus. The newly developed primers reported here constitute a useful tool for genetic population analyses on D. turdina and, potentially, other related species.
Molecular Ecology …, 2002
The Bang's leaf-nose bat, Hipposideros turpis, is an endangered cave-dwelling species inhabiting the southwesternmost islands of Japan. We isolated six dinucleotide microsatellite markers from the partial genomic library of the bat, and examined their allelic variation using a sample (N = 33) from the largest colony in Japan. All the loci showed a moderate allelic variation ranging from two to eight alleles, with the observed heterozygosities from 0.33 to 0.73, and conformed to Hardy–Weinberg expectations. The present microsatellite markers will be useful in assessing population genetic structure and gene flow among populations of this species.
African Journal of Biotechnology, 2013
Microsatellite loci for Chaerephon pumilus sensu lato from south eastern Africa were cross-amplified using primers developed for the Mexican free-tailed bat, Tadarida brasiliensis. Two dinucleotide and four tetranucleotide loci were recovered and genotyped for 74 bats, yielding 9 to 15 alleles per locus. The observed and expected heterozygosities were 0.06 to 0.84 and 0.54 to 0.81 respectively, and the PIC values ranged from 0.51 to 0.80, indicative of considerable variability within the sample. There was no evidence of linkage disequilibrium among pairs of loci, or of deviation from Hardy-Weinberg equilibrium. These six loci were informative in studies of population genetic structure of C. pumilus sensu lato.
Journal of Heredity, 2019
Most species of bats give birth to only 1 pup each year, although Eastern red bats (Lasiurus borealis) can produce up to 5 pups per litter. Offspring in a single litter have been documented to be at different stages of development, suggesting that multiple paternity occurs. We tested the null hypothesis of genetic monogamy in red bats using 6 autosomal microsatellites and 1 X-linked microsatellite from 31 parent/offspring groups for a total of 128 bats. We sampled both pregnant females and mothers with pups that were obtained from bats submitted to departments of health in Oklahoma and Texas for rabies testing. Multiple paternity was assessed using a maximum-likelihood approach, hypothesis testing, and X-linked locus exclusion. The mean polymorphic information content of our markers was high (0.8819) and combined non-exclusion probability was low (0.00027). Results from the maximum-likelihood approach showed that 22 out of 31 (71%) parent/offspring groups consisted of half siblings,...
Conservation Genetics …, 2010
Twenty microsatellite loci developed for the straw-colored fruit bat, Eidolon helvum, are described. These markers were used in multiplex PCRs to amplify genomic DNA from 142 individuals sampled from nine populations across Africa. Nineteen loci were polymorphic, with a mean number of alleles per locus of 16.2 (3-46). Observed and expected heterozygosity values ranged from 0.01 to 0.90 and 0.03 to 0.97, respectively. These markers will be used in combination with mitochondrial DNA sequences to investigate gene flow and the genetic metapopulation structure of E. helvum.
Molecular Ecology …, 2007
The paper presents multiplex panels of polymorphic microsatellites for two closely related cryptic species Pipistrellus pipistrellus and Pipistrellus pygmaeus . We tested the crossspecies amplification of 34 microsatellite loci, originally developed for five vespertilionid bat species. Ten and nine polymorphic loci in P . pipistrellus (mean number of alleles per locus = 10.5) and P . pygmaeus (8.1), respectively, in three multiplex polymerase chain reactions per species were amplified. All loci can be analysed in a single fragment analysis and can be used as markers to the study of evolution and the ecology of structured populations of socially living bats.
Molecular Biology and …, 1988
The Neotropical fruit bat, Artibeus jumaicensis, occurs throughout Latin America and on many islands in the Caribbean. Populations from Jamaica (in the Greater Antilles) to Barbados (in the Lesser Antilles) have been classified as a subspecies (A. j. jumaicensis) separate from that on the Lesser Antillean island of St. Vincent (A. j. schwartzi). Mitochondrial DNA (mtDNA) was isolated from 54 individuals collected on these islands, analyzed by digestion with restriction endonucleases, and the restriction sites were mapped. Three different mtDNA genotypes (16,000 + 200 bp) were identified: J-l (16 animals from Jamaica, one from St. Vincent, 15 from Barbados), J-2 (two animals from Jamaica), and SV-1 (18 animals from St. Vincent, two from Barbados). The J-l and J-2 genotypes were estimated to differ from each other by only 0.4%, but the SV-1 genotype differed from J-l and J-2 by 8.1%-10.5%. The estimated sequence divergence between SV-1 and J-l is unusually large for mammals that are regarded as conspecific. Restriction mapping showed that the differences among the genotypes (presence or absence of particular restriction sites) were located throughout the genome. The presence of the J-1 mtDNA genotype on Jamaica and on St. Vincent and Barbados (1,400 km away) demonstrates that maternal lineages in these bats are not necessarily confined to single islands or limited geographic regions. The presence of the J-l mtDNA genotype within the A. j. schwartzi population on St. Vincent and the presence of the SV-1 genotype in two specimens of A. j. jumaicensis from Barbados document genetic exchange between subspecific populations on these islands, which are separated by 180 km of open water.