Spectrophotometric Quantification of Anti-Inflammatory Drugs by Application of Chromogenic Reagents (original) (raw)
Turkish Journal of Pharmaceutical Sciences
spectrophotometric methods have been developed and validated for quantification of Lornoxicam (LOR) and Mesalamine (MES) drugs in pure form and in pharmaceutical formulations. MATERIALS AND METHODS: Shimadzu Double-beam UV-Visible Spectrophotometer 1800 having spectral bandwidth of 0.1 nm with wavelength accuracy ±0.1 nm and a pair of 1 cm path length matched quartz cells were used to measure absorbance of the resulting solution. Method (I) is used for the quantification of LOR which is based on the measurement of absorbance of bluish green coloured chromogen complex at 760 nm which is formed by reaction of LOR with ferric chloride and potassium ferricyanide (redox technique). Method (II) is used for the quantification of MES that is based on measurement of absorbance of yellow coloured chromogen at 400 nm which is formed by the condensation reaction of the primary amino group of MES with salicylaldehyde reagent (SA) (Schiff base formation). RESULTS: Both the methods obeyed Beer's law in concentration range of 0.5-4.5 μg/mLand 0.2-1.7 μg/mL with good correlation coefficients of 0.9974 and 0.998 for Methods (I) and (II) respectively. CONCLUSION: The developed method is simple, sensitive, specific which is validated statistically as per ICH guidelines and can be used in routine analysis of LOR and MES pharmaceutical dosage forms.
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