Indirect Manganese Removal by Stenotrophomonas sp. and Lysinibacillus sp. Isolated from Brazilian Mine Water (original) (raw)
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Water
Many global mining activities release large amounts of acidic mine drainage with high levels of manganese (Mn) having potentially detrimental effects on the environment. This review provides a comprehensive assessment of the main implications and challenges of Mn(II) removal from mine drainage. We first present the sources of contamination from mineral processing, as well as the adverse effects of Mn on mining ecosystems. Then the comparison of several techniques to remove Mn(II) from wastewater, as well as an assessment of the challenges associated with precipitation, adsorption, and oxidation/filtration are provided. We also critically analyze remediation options with special emphasis on Mn-oxidizing bacteria (MnOB) and microalgae. Recent literature demonstrates that MnOB can efficiently oxidize dissolved Mn(II) to Mn(III, IV) through enzymatic catalysis. Microalgae can also accelerate Mn(II) oxidation through indirect oxidation by increasing solution pH and dissolved oxygen produ...
This study presents the isolation and screening of manganese (II) oxidizing bacteria from wastewater samples of electroplating industrial effluent and its application as a potential biosorbent to remove Mn(II) ions from aqueous solution in a batch system. A statistical approach, the response surface methodology is used to determine the optimum conditions for the generation of biogenic manganese oxides and manganese removal using manganese oxidizing bacterial strain Mn 21. Based on the statistical analysis; the maximum biogenic manganese oxide formation and manganese removal was obtained 64.90% and 96.90% at pH 8, temperature 300C and 10 days incubation time. We can achieve a maximum removal and Mn oxide formation upto 108.9% and 71.1% respectively at optimal conditions of pH 8.0, temperature 31.70C and incubation time of 9.7 days having maximum desirability. The analysis of variance (ANOVA) of Box–Behnken design showed that the proposed quadratic model fitted experimental data very well with coefficient of correlation r2 to be 0.9821, 0.9744 for manganese removal and manganese oxide formation respectively.
Applied Sciences, 2021
Packed bed bioreactors were used to remove soluble manganese from a synthetic mine water as the final stage of an integrated bioremediation process. The synthetic mine water had undergone initial processing using a sulfidogenic bioreactor (pH 4.0–5.5) which removed all transition metals present in elevated concentrations (Cu, Ni, Zn and Co) apart from manganese. The aerobic bioreactors were packed with pebbles collected from a freshwater stream that were coated with black-colored, Mn(IV)-containing biofilms, and their capacity to remove soluble Mn (II) from the synthetic mine water was tested at varying hydraulic retention times (11–45 h) and influent liquor pH values (5.0 or 6.5). Over 99% of manganese was removed from the partly processed mine water when operated at pH 6.5 and a HRT of 45 h. Molecular techniques (clone libraries and T-RFLP analysis) were used to characterize the biofilms and identified two heterotrophic Mn-oxidizing microorganisms: the bacterium Leptothrix discoph...
The Effect of Mn2+ Concentration on Mn Removal by a Sulfate Reducing Bacteria Bioreactor
MATERIALS TRANSACTIONS, 2004
In the design of sulfate reducing bacteria (SRB) bioreactors to remove metal ions from acid mine drainage, the tolerance of bacteria to metal ions is an important factor. The present study investigated the effects of Mn 2þ concentration on the growth and Mn removal activity of SRB in mediums containing 100$600 gÁm À3 Mn 2þ. The Mn 2þ adsorbed on SRB cells and weakened the ability of bacterial sulfate reduction. However, the suppressive effect of Mn 2þ on SRB growth can be disregarded, and 200 gÁm À3 of Mn 2þ in solution was removed, satisfying the requirement of the maximum contaminant level (MCL) of environmental water quality in Japan.
The Effect of Mn<SUP>2+</SUP> Concentration on Mn Removal by a Sulfate Reducing Bacteria Bioreactor
Materials transactions, 2004
In the design of sulfate reducing bacteria (SRB) bioreactors to remove metal ions from acid mine drainage, the tolerance of bacteria to metal ions is an important factor. The present study investigated the effects of Mn 2þ concentration on the growth and Mn removal activity of SRB in mediums containing 100$600 gÁm À3 Mn 2þ. The Mn 2þ adsorbed on SRB cells and weakened the ability of bacterial sulfate reduction. However, the suppressive effect of Mn 2þ on SRB growth can be disregarded, and 200 gÁm À3 of Mn 2þ in solution was removed, satisfying the requirement of the maximum contaminant level (MCL) of environmental water quality in Japan.
Water Research, 2010
The interaction of chemical, physical and biological factors that affect the fate, transport and redox cycling of manganese in engineered drinking water systems is not clearly understood. This research investigated the presence of Mn-oxidizing and-reducing bacteria in conventional water treatment plants exposed to different levels of chlorine. Mn (II)-oxidizing and Mn(IV)-reducing bacteria, principally Bacillus spp., were isolated from biofilm samples recovered from four separate drinking water systems. Rates of Mnoxidation and-reduction for selected individual isolates were represented by pseudo-firstorder kinetics. Pseudo-first-order rate constants were obtained for Mn-oxidation (range: 0.106e0.659 days À1), aerobic Mn-reduction (range: 0.036e0.152 days À1), and anaerobic Mnreduction (range: 0.024e0.052 days À1). The results indicate that microbial-catalyzed Mnoxidation and-reduction (aerobic and anaerobic) can take place simultaneously in aqueous environments exposed to considerable oxygen and chlorine levels and thus affect Mnrelease and-deposition in drinking water systems. This has important implications for Mnmanagement strategies, which typically assume Mn-reduction is not possible in the presence of chlorine and oxidizing conditions.
Manganese: Its Speciation, Pollution and Microbial Mitigation
International Journal of Applied Sciences and Biotechnology, 2013
Manganese is known to be one of the essential trace elements and has plenty of applications. Inspite of its essential nature, concerns are arising due to its toxic nature at higher concentration. Several methods of removing manganese from environment have been proposed during the last few decades. However, the most favourable option based on cost-effectiveness, performance, and simplicity is still under investigation. The current review summarizes updated information on various technical aspects on manganese, including chemical nature, speciation, toxicity and remediation strategies. The review starts with covering the major sources of manganese, its interaction with biological biomolecules causing toxicity. This is followed by its speciation in environment, describing both biotic and abiotic processes. The biotic processes describe the role of microorganisms in the oxidation/ reduction of various oxidation states of manganese. Whereas, abiotic processes mainly describes the role of...
Fundamental Study on the Removal of Mn2+ in Acid Mine Drainage using Sulfate Reducing Bacteria
Materials transactions, 2004
The optimum conditions for Mn 2þ removal from acid mine drainage was studied by a SRB (sulfate reducing bacteria) bioreactor. Chemical experiments with Na 2 S as a S 2À source were conducted to investigate the effects of pH, coexisting metal ions, and the components in a growth medium for SRB on MnS formation from Mn 2þ solutions. The amount of Mn removed from the Mn 2þ solutions decreased with decreasing pH. The Zn 2þ or Fe 2þ coexisting in the solutions consumed S 2À by forming ZnS or FeS, and this inhibited Mn removal. Sodium citrate, a component of the growth medium for SRB, formed a complex with Mn 2þ and suppressed MnS formation. Biological experiments using the SRB reactor were carried out at 37 C and it was confirmed that the Mn 2þ concentration decreased to less than 10 gÁm À3 from 100 gÁm À3 at neutral pHs (pH 5-7) after 100 hours when other metal ions and sodium citrate were absent. The formed precipitate was identified to be metastable-MnS with a band gap of about 3.8 eV by XRD, XRF, and UV-VIS.
Simultaneous removal of iron and manganese from acid mine drainage by acclimated bacteria
Journal of Hazardous Materials, 2020
A bacterial consortium for efficient decontamination of high-concentration Fe-Mn acid mine drainage (AMD) was successfully isolated. The removal efficiencies of Fe and Mn were effective, reaching 99.8 % and 98.6 %, respectively. High-throughput sequencing of the 16S rRNA genes demonstrated that the microbial community had changed substantially during the treatment. The Fe-Mn oxidizing bacteria Flavobacterium, Brevundimonas, Stenotrophomonas and Thermomonas became dominant genera, suggesting that they might play vital roles in Fe and Mn removal. Moreover, the pH of culture increased obviously after incubation, which was benefit for depositing Fe and Mn from AMD. The specific surface area of the biogenic Fe-Mn oxides was 108-121 m 2 /g, and the surface contained reactive oxygen functional groups (-OH and −COOH), which also improved Fe and Mn removal efficiency. Thus, this study provides an alternative method to treat AMD containing high concentrations of Fe and Mn. 1. Introduction Acid mine drainage (AMD) is severely threatening the safety of aquatic and terrestrial ecosystems (Aguinaga et al., 2018; Auld et al., 2017). Available methods for AMD treatment include traditional neutralization techniques, magnetic nanoparticles, lignite and zeolite, and membrane methods, among others (Rand and Ranville, 2019). Traditional neutralization techniques require additions of commercially produced alkali, which is expensive and generate a large volume of sludge, especially in AMD that contains high levels of iron/ferrous ions