COBALT OXIDE NANOPARTICLES: SYNTHESIS AND CHARACTERIZATION (original) (raw)
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Optimisation of Cobalt Oxide Nanoparticles Synthesis as Bactericidal Agents
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AIM: With the increased bacterial resistance and the resulting problems in recent years, it seems necessary to find new biocompatible compounds to confront this problem. This research was conducted to optimise the synthesis of cobalt oxide nanoparticles with the highest antibacterial activity. METHODS: In the present study, 9 experiments were designed using the Taguchi method. The effect of three factors of cobalt nitrate, KOH and the stirring time in the synthesis of cobalt oxide nanoparticles with the highest antibacterial activity was investigated. The bactericidal effect of synthesised nanoparticles was evaluated using the colony-forming unit (CFU) and disk diffusion methods. The characteristics of nanoparticles were studied using the Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and the scanning electron microscopy (SEM). RESULTS: The results indicated that all three evaluated factors were effective on the antibacterial properties of the synthesised na...
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Materials Research Express, 2020
Cobalt oxide nanoparticles (Co3O4-Nps) have many applications and now a days the green methods of synthesis of these NPs are preferred over other methods because of associated benefits. In this study, Co3O4-Nps were synthesized by using leaves extract of Populus ciliata (safaida) and cobalt nitrate hexa hydrate as a source of cobalt. The synthesized NPs were analyzed by different techniques such as fourier transform spectroscopy (FTIR), x-ray diffraction (XRD), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Antibacterial activities of the synthesized Co3O4-Nps were evaluated against gram negative and gram positive bacteria and found active against Escherichia coli (E. coli), Klebseilla pneumonia (K. pneumonia), Bacillus subtillus (B.subtillus) and Bacillus lichenifermia (B. lichenifermia). The activity results were analyzed statistically by one-way ANOVA, with ‘Dunnett’s Multiple Comparison Test’. The maximum mean activity (21.8 ± 0.7) was found for B...
Size- dependent Antibacterial Activity of Cobalt Oxide Nanoparticles
2015
Dedication iii Acknowledgment iv List of Symbols v Table of Contents vi List of Figures viii List of Tables x Abstract xiii Chapter 1 Introduction 1 1.1 Motivation and Project Aims 1 Chapter 2 State of Art 8 2.1 Nanotechnology 8 2.2 Stabilizers 2.3 Cobalt oxide Nanoparticles 2.4 Size-dependent antibacterial activity of nanoparticles 2.5 Bacterial strains 2.5.1 Staphylococcus aureus 2.
Synthesis , characterization and antibacterial properties of nano-sized cobalt particles *
2016
Nano-sized particles exhibit unique and fascinating physical, chemical and biological properties owing to their large surface to volume ratio. The study of nanoparticles interaction with microorganisms is of great importance to consider them for various biomedical applications. The aim of the present study was to study the antibacterial behavior of cobalt oxide (Co2O4) nanoparticles against gram positive and gram negative bacterial strains. Solution based chemical reduction method was used to synthesize the Co2O4 nanoparticles taking cobalt chloride as precursor. The prepared Co2O4 nanoparticles were characterized by X-ray diffraction (XRD) and Variable Sample Magnetometer (VSM) techniques to study the structural and magnetic properties. Kirby-Bauer disk diffusion susceptibility method was used to investigate the in-vitro antimicrobial activity of cobalt nanoparticles. Escherichia coli (E.coli), Pseudomonas aeruginosa (P. aeruginosa) and Bacillus subtilis (B. subtilis) were used to ...
Surface-modified cobalt oxide nanoparticles: new opportunities for anti-cancer drug development
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The development of smart nanoparticles that can exhibit the anti-cancer activity, introduces better efficacy and lower toxicity for treatment. The present study was aimed to evaluate the anti-cancer activity of surface functionalized CoO nanoparticles against Jurkat (T-cell lymphoma) and KB (oral carcinoma) cell lines. The nanosized cobalt oxide nanoparticles (CoO) was prepared by thermal decomposition method followed by surface modification using phosphonomethyl iminodiacetic acid (PMIDA). The PMIDA-coated CoO nanoparticle was characterized by X-ray diffraction, dynamic light scattering, and transmission electron microscopy; and the conjugation was analyzed by Fourier transform infrared spectroscopy. The resultant nanoparticles with an average size less than 100 nm measured by dynamic light scattering and transmission electron microscopy. Cytotoxicity study, flow cytometric analysis and scanning electron micrographs have been revealed that PMIDA-coated nanoparticles significantly enhances the cellular uptake of the nanoparticle and thus facilitates apoptosis of cancer cell (Jurkat and KB). For the application of PMIDA-coated CoO nanoparticles in the medical field, doxorubicin, a potent anti-cancer drug, has been used in similar fashion in this experimental design and all these effects or patterns were observed.
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Journal of Nanostructure in Chemistry, 2013
This study has investigated the different factors in obtaining cobalt oxide nanoparticles. The factors that have been studied are the effect of pH, synthesis temperature, capping agent, annealing temperature, and different usage of cobalt salts. Cobalt oxide nanoparticles were characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, UV, Fourier transform infrared spectroscopy, and X-ray diffraction. In order to find the effects of various factors, the optimum pH was achieved in the first experiment by the precipitation method; after finding out that in experiments with pH 8 to 9, the particles are homogeneous in shape and regular. The other experiments were repeated with the same pH but changing the factors. In the second experiment, the effect of synthesis temperature at 40°C, 60°C, 80°C, and 100°C was investigated. To find out the effect of capping agent, two experiments were done, one using the oleic acid and the other using 2-(dodecyloxy) acetic acid. In the case of investigating the annealing temperature, the black cobalt oxides were annealed at 400°C, 500°C, and 600°C. The last one was done using two different cobalt salts, cobalt nitrate and cobalt sulfate. Finally, cobalt oxide nanoparticles were characterized.
Biosynthesis of Cobalt Oxide Nanoparticles - A Short Review
Volume 5, Issue 2, 2019
This short review article presents a summary of ecofriendly ways to synthesize cobalt oxide (Co3O4) nanoparticles using biomaterials like plant extract, bacterium and fungus. Currently, researchers have focused their concentration on the biosynthesis of nanoparticles using biomaterials containing phenols, carbonyls, amines, carbohydrates, proteins and amino acids which act as bio-templates, reducing agents, stabilizing agents, capping agents and chelating agents for nanoparticles. This article also discussed the reports of characterization of cobalt oxide nanoparticles by UV-Vis spectroscopy, diffuse reflectance spectroscopy (DRS), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), selected area electron diffraction (SAED), scanning electron microscopy (SEM), high resolution scanning electron microscopy (HRSEM), transmission electron microscopy (TEM), high resolution transmission electron microscopy (HRTEM), atomic force microscopy (AFM) and Brunauer-Emmett-Teller (BET) analyzer which were carried out by various researchers. The applications of these biosynthesized cobalt oxide nanoparticles in a wide range of potential zones are listed including antibacterial activity, photo catalyst, sensor and supercapacitor.
Agronomy, 2021
The development of sustainable, ecofriendly, and cost-effective methods for the synthesis of nanomaterials is an important aspect of nanotechnology these days. The present study was aimed at synthesizing cobalt oxide (Co3O4) nanoparticles by using plant extracts of Aerva javanica, bacterial isolates from rhizospheric soil of Potentilla atrosanguinea, Swertia petiolata, Senecio chrysanthemoides, and from fungus Fusarium oxysporum. X-ray diffraction spectroscopy (XRD) and scanning electron microscopy (SEM) techniques were used in the characterization of the synthesized nanoparticles. The bacterial strain, Bacillus subtilis, isolated from rhizosphere of Potentilla atrosanguinea (N1C1), Fusarium oxysporum, methanolic and aqueous extracts of Aerva javanica reduced the cobalt salts to cobalt oxide nanoparticles. The nanoparticles, synthesized from bacterial isolate N1C1 (Bacillus subtilis) and from Fusarium oxysporum had average particle size of 31.2 nm and 33.4 nm, respectively, whereas,...
Evaluating the antibacterial effect of cobalt nanoparticles against multi-drug resistant pathogens
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This study aimed to estimate the effect of cobalt nanoparticles (Co NPs) with different concentrations against multidrug-resistant (MDR) pathogenic bacteria. Three isolates of (gram-positive), . (gram-negative), and (gram-negative) bacteria were extracted from various clinical examples utilizing routine methods on bacteriological culture media. The antibacterial sensitivity of commercial antibiotics such as Ciprofloxacin, Cefotaxime, Gentamycin, and Amoxicillin was broken down on a Muller Hinton agar plate and evaluated using the disk diffusion method. The study results demonstrated the antibacterial effect of the Co NPs against the bacterial isolates with three different concentrations utilized in the study. The results indicated that the Co NPs showed the highest antibacterial activity when utilizing 100 μg/ml against followed by and with zones of inhibition measured as 22.2±0.1 mm, 20.3±0.15 mm, and 15.8±0.1 mm; respectively. Co NPs at a 100 μg/mL concentration showed higher inhi...
Comparative Effects of Particle Sizes of Cobalt Nanoparticles to Nine Biological Activities
International Journal of Molecular Sciences
The differences in the toxicity of cobalt oxide nanoparticles (Co-NPs) of two different sizes were evaluated in the contexts of the activities of bacterial bioluminescence, xyl-lux gene, enzyme function and biosynthesis of β-galactosidase, bacterial gene mutation, algal growth, and plant seed germination and root/shoot growth. Each size of Co-NP exhibited a different level of toxicity (sensitivity) in each biological activity. No revertant mutagenic ratio (greater than 2.0) of Salmonella typhimurium TA 98 was observed under the test conditions in the case of gene-mutation experiments. Overall, the inhibitory effects on all five bacterial bioassays were greater than those on algal growth, seed germination, and root growth. However, in all cases, the small Co-NPs showed statistically greater (total average about two times) toxicity than the large Co-NPs, except in shoot growth, which showed no observable inhibition. These findings demonstrate that particle size may be an important phy...