Phytochemical Screening, In-vitro and In-vivo Anti-diabetic Activity of Nelumbo nucifera Leaves against Alloxan-Induced Diabetic Rabbits (original) (raw)
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International Journal of Pharmacy and Pharmaceutical Sciences, 2017
Objective: The main objective of the study was to investigate the effect of ethanol seed extracts of Nelumbo nucifera Gaertn (Nymphaeaceae) as antihyperglycemic, anti-hyperlipidemic and antioxidant activity in streptozotocin-induced diabetic rats. Methods: Diabetes was induced in wistar albino rats by administration of streptozotocin (single intraperitoneal dose of 60 mg/kg B. W). The ethanol extract of N. nucifera seed at a dose of 200 and 400 mg/kg body weight was administrated at a single dose per day to STZ induced diabetic rats for a period of 42 d. The different pharmacological parameters were evaluated. The effect of ethanol seed extract of N. nucifera on insulin, blood glucose, urea, creatinine, HbA1C, serum protein, albumin, globulin, serum enzymes, serum lipid profiles, lipid peroxides (LPO) and other antioxidant enzymes like catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and reduced glutathione (GSH) were measured in the diabetic rats. Results: In the acute toxicity study ethanol seed extract of Nelumbo nucifera were non-toxic at 2000 mg/kg in rats. The ethanol extract of Nelumbo nucifera seed showed significant reduction in blood glucose (p<0.05), serum enzymes (SGPT, SGOT, ALP) (p<0.05), lipid parameters (TC, TG, VLDL, LDL) (p<0.05) except HDL and significantly increased insulin (p<0.05), HDL (p<0.05), GPx, GSH, SOD and CAT (p<0.05) at the dose of 400 mg/kg when compared with the diabetic-induced control. Conclusion: The present study suggested that the NNSE has significant (p<0.05) antihyperglycemic, antihyperlipidemic and antioxidant activity in STZ induced diabetic rats. These results clearly indicate that Nelumbo nucifera is effective against free radical-mediated diseases, thus replacing the synthetic ones.
Unveiling the Therapeutic Potential: Antidiabetic Activity of Nelumbo nucifera
Texila International Journal of Public Health, 2024
The escalating prevalence of diabetes mellitus on a global scale has spurred an urgent need for innovative and effective therapeutic interventions, Nelumbo nucifera, commonly known as the sacred lotus, has been revered for centuries in various cultures for its symbolic significance and aesthetic appeal. Beyond its cultural importance, various parts of the Nelumbo nucifera plant, including its seeds, leaves, and rhizomes, are rich repositories of bioactive compounds. These compounds, ranging from alkaloids and flavonoids to polysaccharides, exhibit diverse pharmacological activities and its been a subject of interest for researchers due to their rich phytochemical composition. Among its various bioactive properties, the alpha-glucosidase and amylase inhibition capabilities stand out for their potential in managing diabetes mellitus. These enzymes play crucial roles in carbohydrate metabolism, and their inhibition can effectively control postprandial hyperglycemia, a critical aspect of diabetes management This study is done using different concentrations of samples and standard drugs showing amylase and alpha-glucosidase inhibition properties and analysed and calculated using percentage inhibition formula and it showed the percentage of alpha amylase inhibition by standard concentration 400ug is 53.3 % and sample 500ug is 50%, The percentage inhibition of concentration alpha glucosidase inhibition for standard concentration 400ug is 72.2% and sample 500ug is 71.4%. The study concluded that Nelumbo nucifera possesses both alpha-glucosidase and amylase inhibition properties and is a good natural source of remedy for diabetes, that can be combined with other drugs.
Journal of Diabetes Mellitus
The study was carried out to evaluate the hypolipidemic and hypoglycemic effect of Nelumbo nucifera leaf powder. In rats, hyperlipidemia was induced by feeding High Fat Diet (Lab diet: Dalda: Coconut oil = 4:3:1) and type 2 diabetes was built inside by injecting Alloxan. The sample N. nucifera leaf powder was added in different percentages with the regular Lab Diet for 21 days feeding. In case of hyperlipidemia developed groups, feeding of Nelumbo nucifera leaf powder at, 30% (70% Lab diet + 30% Sample), 20% (80% Lab diet + 20% Sample) and 10% (90% Lab diet + 10% Sample) showed varied but in a nutshell significant (p < 0.05) decrease in serum total cholesterol, triglyceride and LDL-Cholesterol levels when compared to control group while HDL-Cholesterol level was augmented significantly (p < 0.05). Daily feeding of Nelumbo nucifera leaf powder for 21 days resulted significant decrease in the blood glucose levels of alloxan-induced diabetic rats. Both the percentages of Nelumbo nucifera leaf powder having 20% with 80% Lab Diet and 10% with 90% Lab Diet significantly (p < 0.05) decreased blood glucose level up to 44% and 33% respectively where the higher percentage of Nelumbo nucifera powder was found to be exerted to the more prominent effects in lowering the blood glucose level. Nelumbo nucifera leaf powder had 75% efficacy rate in lowering the blood glucose level in comparison to the drug control group treated with glibenclamide which was found with the decreasing capability up How to cite this paper:
Effect of Nelumbo nucifera rhizome extract on blood sugar level in rats
Journal of Ethnopharmacology, 1997
Oral administration of the ethanolic extract of rhizomes of Nelumbo nucifera markedly reduced the blood sugar level of normal, glucose-fed hyperglycemic and streptozotocin-induced diabetic rats, when compared with control animals. The extract improved glucose tolerance and potentiated the action of exogenously injected insulin in normal rats. When compared with tolbutamide, the extract exhibited activity of 73 and 67% of that of tolbutamide in normal and diabetic rats, respectively.
Therapeutic Efficacy of Lotus (Nelumbo nucifera) Leaves Extract to Manage Diabetes mellitus
Journal of Advanced Zoology
Natural bioactive compounds that target pancreatic cells for the prevention and treatment of diabetes mellitus are gaining popularity. The purpose of this research was to investigate the physiologic effects of Lotus leaves on insulin and sugar levels in order to improve diabetes control. The Soxhelt apparatus was used to extract the Lotus ethanol extract, which was diluted with distilled water at two concentrations: 5 mg/ml (5 percent) and 10 mg/ml (10 percent).. Thirty female white albino rats were randomly separated into three groups: G1(n=10) was given distilled water 1ml, G2(n=10) and G3(n=10) were given a single dosage of 5% and 10% extract solution, respectively, daily for ten days. The results showed significant decrease of glucose level in serum after 10 days of treatment G2= 87.36±1.5 and G3=69.7±1.7 and significant increase of insulin level in serum G2= 78.92±1.03 and G3= 89.36±1.46.
Invivo Antidiabetic evaluation of Neem leaf extract in alloxan induced rats
Journal of Applied …, 2011
Azadirachta indica has been used medicinally throughout history by many different cultures. Many compounds have been found in the exudates of the, Azadirachta indica plant that have been used medically by humans. We have examined the pharmacological hypoglycemic action of Azadirachta indica in diabetic rats. After treatment for 24 hrs, Azadirachta indica 250mg/kg (single dose study) reduced glucose (18%), cholesterol (15%), triglycerides (32%), urea (13%), creatinine (23%), and lipids (15%). Multiple dose study for 15days also reduced creatinine, urea, lipids, triglycerides and glucose. In a glucose tolerance test in diabetic rats with neem extract 250 mg/kg demonstrated glucose levels were significantly less compared to the control group. , Azadirachta indica significantly reduce glucose levels at 15th day in diabetic rats. Azadirachta indica serves as an important alternative source in the management of diabetes mellitus involved in reducing increased blood glucose during diabetes which should be examined further by oral hypoglycemic therapy.
The blood glucose lowering effects of the aqueous leaf extracts of Cassia alata, Acalypha torta and Breynia nivosa, and aqueous root extracts of Daniellia oliveri and Nauclea latifolia traditionally employed in Nigeria in the management of diabetes mellitus were compared in alloxan-induced diabetic Wistar rats. The same dose, 100.0 mg/kg body wt. of each extract was used. Alloxan (150.0 mg/kg body wt.), extracts, and the reference drug, glibenclamide (20.0 mg/70kg body wt.) were solubilized in normal saline and administered intraperitoneally. Investigation into the mechanisms of action of the most potent extract was carried out by determining it's effects on lipid-peroxidation, superoxide dismutase (SOD), catalase and glucokinase. The extracts caused significant (p< 0.01) reductions in the blood glucose concentrations of the diabetic animals, thereby validating their antidiabetic properties. The order of potency was Nauclea latifolia (79.9%) > Acalypha torta (66.5%) > Breynia nivosa (50.4%) > Cassia alata (39.2%) > Daniellia oliveri (31.9%). The most active extract, Nauclea latifolia, was more potent than glibenclamide. N. latifolia extract decreased malonylaldehyde concentration and superoxide dismutase activity, although these effects were not statistically significant, whereas significant reduction in catalase activity was noted. Alteration of body's oxidant-antioxidant balance may be enhancing the antidiabetic activity of Nauclea latifolia root. Glucokinase activity was also significantly (p<0.05) elevated.
Biological trace element research
The seeds of Nelumbo nucifera (Lotus) have been used in the traditional system of medicine for various ailments including diabetes. The present study was aimed at analyzing the levels of biologically important trace elements in the lotus seeds by atomic absorption spectroscopy and evaluating the hypoglycemic properties of seed ash on streptozotocin-induced diabetes in rats. Diabetic rats treated with lotus seed ash at a concentration of 200 mg/kg body weight orally for 30 days exhibited significant hypoglycemic activity. The presence of trace elements in appreciable amounts in the seeds may play a direct or indirect role on insulin secretion or its action in a synergetic manner. The hypoglycemic activity of the ash was comparable with glyclazide. The role of trace elements in disorders related to diabetes is also discussed briefly.
Antidiabetic potential of medicinal plants
Acta poloniae pharmaceutica
It is the fact that diabetes can't be cured and it has never been reported that someone had recovered totally from diabetes. The rapidly increasing incidence of diabetes mellitus is becoming a serious threat to mankind health in all parts of the world. Moreover, during the past few years some of the new bioactive drugs isolated from plants showed antidiabetic activity with more efficacy than oral hypoglycemic agents used in clinical therapy. The traditional medicine performed a good clinical practice and is showing a bright future in the therapy of diabetes mellitus. The present paper reviews natural medicines with their mechanism of action and their pharmacological test results. Many studies have confirmed the benefits of medicinal plants with hypoglycemic effects in the management of diabetes mellitus. The effects of these plants may delay the development of diabetic complications and correct the metabolic abnormalities. WHO has pointed out this prevention of diabetes and its ...