Heterogeneity of rat macrophages recognized by monoclonal antibodies: an immunohistochemical and immunoelectron microscopic study (original) (raw)
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Journal of Leukocyte Biology, 1987
An anti.macrophage monoclonal antibody designated TRPM 3 was produced using thioglycoltate-ellclted rat peritoneal macrophages as immunogen. By the immunoper. oxidase method, TRPM-3 was found to be specific for certain macrophage populations, such as marginal zone macrophages and marginal metallophils of the spleen, sinus macrophages of the lymph nodes, and omentum macrophages. No epithelial cells, peripheral blood monocytes, granulocytes, or lymphocytes had reacted with TRPM.3. immunoelectron microscopically, reaction to TRPM.3 was clearly demonstrated on the plasma membrane of splenic marginal zone macrophages, lymphatic sinus macro phages, omentum macrophages, and pentoneal macrophages. Accordingly, TRPM-3 was considered to have recognized the particular membrane antigen expressed by restricted macrophage populations.
Journal of Experimental Medicine, 1983
The immune response genes of the major histocompatibility gene complex code for surface antigens (Ia) now believed to be involved in the interaction between T cells and accessory or "antigen-presenting" cells (1, 2). Whilst some groups suggest that Ia antigens present on subpopulations of mononuclear phagocytes are involved in T cell activation (1-4), others have presented evidence for the involvement of a separate Ia + cell population (5-8) now generally referred to as dendritic cells. The in vivo correlate of the isolated antigen-presenting dendritic cell is not yet clear. The availability of a specific monoclonal antibody against mouse dendritic cells (9) will be helpful in clarifying this question. One obvious candidate is the Ia + "interdigitating cell" that has been described in thymus and in T cell-dependent areas of lymphoid organs (10-15). However, whilst the isolated splenic dendritic cell has virtually none of the functional characteristics of a mononuclear phagocyte (6), it has been suggested that interdigitating cells are members of the mononuclear phagocyte system and are related to epidermal Langerhans cells and similar cells .found in afferent lymphatics .
A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes
Cell and Tissue Research, 1986
A monoclonal antibody (BM8) raised in the rat against cultured mouse bone marrow-derived macrophages reacted only with macrophages and not with granulocytes, mast cells, platelets, lymphocytes, fibroblasts, and endothelial cells. BM8 did not detect blood monocytes. In cultured bone-marrow cells, expression of BM8-antigen was found on a few macrophages after one day of culture and reached its maximum level with 80% positive macrophages after 7-10 days of culture. The antibody BM8 belonged to the IgG2a subclass, was non-cytotoxic and directed against a 125 kD membrane antigen. In cryostat sections of normal mouse tissues (spleen, lymph node, thymus, liver, skin) BM8 detected tissue-fixed macrophages and Langerhans cells in the skin. In spleen and lymph node, BM8 reacted with macrophages in the red pulp and in the medullary cords, respectively, but not with heavily phagocytosing marginal-zone macrophages, as revealed by in-vivo phagocytosis of colloidal carbon. In granulomata induced by complete Freund's adjuvant, BM8 detected inflammatory macrophages but not epithelioid cells. Thus, antibody BM8 detected a differentiation antigen expressed only on mature, tissue-fixed macrophages.
The Journal of …, 1983
The immune response genes of the major histocompatibility gene complex code for surface antigens (Ia) now believed to be involved in the interaction between T cells and accessory or "antigen-presenting" cells (1, 2). Whilst some groups suggest that Ia antigens present on subpopulations of mononuclear phagocytes are involved in T cell activation (1-4), others have presented evidence for the involvement of a separate Ia + cell population (5-8) now generally referred to as dendritic cells. The in vivo correlate of the isolated antigen-presenting dendritic cell is not yet clear. The availability of a specific monoclonal antibody against mouse dendritic cells (9) will be helpful in clarifying this question. One obvious candidate is the Ia + "interdigitating cell" that has been described in thymus and in T cell-dependent areas of lymphoid organs (10-15). However, whilst the isolated splenic dendritic cell has virtually none of the functional characteristics of a mononuclear phagocyte (6), it has been suggested that interdigitating cells are members of the mononuclear phagocyte system and are related to epidermal Langerhans cells and similar cells .found in afferent lymphatics .
Cell & Tissue Research, 1994
We describe ER-HR3, a monoclonal antibody directed against bone marrow-derived haemopoietic reticulum cells. ER-HR3-positive cells have the electronmicroscopic and enzyme-histochemical characteristics of macrophages. Additionally, they are able to phagocytoze. The ER-HR3 antigen is expressed by a majority of blood monocytes and is present on a subpopulation of resident macrophages in multiple organs. ER-HR3-positive cells are abundant in the bone marrow, the splenic red pulp, the mesenteric lymphoid paracortex and the interfollicular areas of the Peyer's patch. Few ER-HR3positive cells have been observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Moreover, epidermal Langerhans cells express the antigen. No cross-reactivity with other cell types has been found. It is concluded that ER-HR3 has a unique distribution pattern distinct from other macrophage-specific antibodies.
Journal of Experimental Medicine, 1975
Culture fluids rich in mononuclear phagocytes have a powerful effect on various in vitro reactions of lymphocytes (1-9). The culture fluids contain a mitogen for thymocytes and mature lymphocytes . Also found are active principles which differentiate memory B cells into antibody-secreting cells and which increase helper activities of T lymphocytes (2, 3). In a previous report we obtained from experiments in which lymphocytes were depleted from the cell preparations, strong evidence that the cellular source of the active component (s) was the macrophage (3). The activity secreted into the medium, however, was variable from experiment to experiment, perhaps related to the state of macrophage activation. In this paper we present results of experiments in which we studied the relation between macrophage activation and/or stimulation with the secretion of the various activities. We have found two ways in which to generate high levels of lymphostimulatory activities in macrophage cultures: one is to add a series of materials that are readily taken up by the macrophages; the second is to add a small number of activated T cells to the macrophage-rich cultures.
Heterogeneity of Mouse Thymic Macrophages: I. Immunohistochemical Analysis
Archives of Histology and Cytology, 1997
As the first step toward understanding the identity and functions of thymic macrophages in situ, we examined the phenotypic heterogeneity of mouse thymic macrophages in tissue sections by the immunohistochemical double staining method with four monoclonal antibodies (F4/80, Mac-2, anti-CD32/16 and anti-I-A antibodies) as macrophage markers. Morphologically, three types of macrophages were identified: dendritic, round and flat-shaped. Dendritic macrophages were scattered throughout the thymus, and most of them were stained by all four markers. Among these macrophages, those at the cortico-medullary region (CMR) expressed a high intensity of CD32/16 antigen. Round macrophages were also distributed throughout the thymus; most of them, however, were localized in the cortico-medullary region to the medulla. These cells were F4/80-negative, Mac-2-positive, CD32/ 16-negative and I-A-positive. In contrast, round macrophages located at the cortex expressed F4/80. Flatshaped macrophages were localized at the subcapsular region of the cortex where active lymphopoiesis was observed. This type was positive for F4/80 and CD32/16, but negative for Mac-2. Furthermore, most of the three types of thymic macrophages showed intense reactions of the I-A antigen within the cytoplasm in addition to the expression of I-A antigen on the cell membrane. These results indicate that morphological characteristics of thymic macrophages at different locations reflect phenotypic variations detected in immunohistochemistry, and suggest that these different type macrophages may play distinct roles at various locations in thymocyte development in the thymus.
European Journal of Immunology, 1976
Studies on the relationships between the immunogenicity and catabolism of antigens and their binding to the surface of macrop hages * The Lautenberg Center for General and Tumor Immunology, Hebrew University-Hadassah Medical School, Jerusalem The relationship between immunogenicity of Shigella paradysenteriae, the branched synthetic polypeptide poly-L (Tyr, Glu)-poly LPro-poly LLys [(T, G)-Pro--L] and human serum albumin (HSA) interacting with macrophages and the kinetics of antigen degradation and degree of binding t o the cell surface was studied. Following thioglycollate inoculation into C57BL/6 mice, the peritoneal-stimulated maciophages had higher leve!s of hydrolases as compared to unstimulated cells. The lysates of the stimulated macrophages catabolized the three labeled antigens faster than did the lysates of unstimulated cells. However, when degradation of labeled antigens by macrophage cells was assessed, n o direct correlation could be demonstrated between the level of cell hydrolases and rate of (T, G)-Pro--L or HSA catabolism.
Journal of Cell Science, 1984
The immune response genes of the major histocompatibility gene complex code for surface antigens (Ia) now believed to be involved in the interaction between T cells and accessory or "antigen-presenting" cells (1, 2). Whilst some groups suggest that Ia antigens present on subpopulations of mononuclear phagocytes are involved in T cell activation (1-4), others have presented evidence for the involvement of a separate Ia + cell population (5-8) now generally referred to as dendritic cells. The in vivo correlate of the isolated antigen-presenting dendritic cell is not yet clear. The availability of a specific monoclonal antibody against mouse dendritic cells (9) will be helpful in clarifying this question. One obvious candidate is the Ia + "interdigitating cell" that has been described in thymus and in T cell-dependent areas of lymphoid organs (10-15). However, whilst the isolated splenic dendritic cell has virtually none of the functional characteristics of a mononuclear phagocyte (6), it has been suggested that interdigitating cells are members of the mononuclear phagocyte system and are related to epidermal Langerhans cells and similar cells .found in afferent lymphatics (14). Apart from T cell activation during an immune response, macrophages have also been implicated in the control of hematopoiesis in the bone marrow (16-20), the differentiation of thymocytes in the thymus (21),. proliferation and keratinization of squamous epithelial cells (Langerhans cells [22-24]), and the antibody response of B lymphocytes to T-independent antigens (25). In order to determine the roles of mononuclear phagocytes and other cells in these systems, it is necessary to have reliable surface markers analogous to those that have delineated subclasses of T lymphocytes (26). F4/80 is a hybridoma that secretes a noncytotoxic rat IgG2b directed against a 160-kdalton plasma membrane antigen of mouse mononuclear phagocytes (27). The antigen is similar in * Supported in part by the Medical Research Council, U. K.