Effects of Inclusion of Polyunsaturated Fatty Acids and Antioxidants on Spermatozoa Morphology of Potchefstroom Koekoek Cockerels (original) (raw)
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Journal of Advanced Agricultural Technologies
The aim of the study was to investigate the effect of dietary fish oil supplementation on the semen characteristics of the Potchefstroom Koekoek cockerels. Thirty cockerels were divided into five groups, kept in individual cages and received five different diets: Commercial layer diet (CL), commercial cockerel diet (CC), modified layer diet (ML), modified layer diet supplemented with 5% fish oil (MLP) and a modified layer diet supplemented with 5% fish oil, 200mg/kg vitamin E and 5mg/kg selenium. Semen was collected twice a week on Mondays and Fridays. Semen characteristics were evaluated. Supplementation of fish oil did not significantly improve semen volume and sperm concentration. However, supplementation with polyunsaturated fatty acids and antioxidants increased the percentage of live and normal, as well as total motility of spermatozoa. The percentage of spermatozoa with mid-piece abnormalities and static motility were reduced in cockerels fed the MLPA diet. The supplementation of polyunsaturated fatty acids and antioxidants was associated with improved semen characteristics. Similarly, the supplementation of antioxidants provided enhanced protection against midpiece abnormalities
Effects of Inclusion of Polyunsaturated Fatty Acids and Antioxidants on Spermatozoa
2015
This study was conducted to determine the effects of increasing dietary polyunsaturated fatty ac-ids (PUFA) and antioxidants (Se and vitamin E) on spermatozoa morphology. At 24 weeks of age, Potchefstroom Koekoek cockerels (n = 60) were selected from the base population. Cockerels were randomly allocated into five dietary treatments with 12 cockerels per dietary treatment. The diets consisted of commercial layer diet (CL), commercial cockerel diet (CC), modified layer diet (ML), modified layer diet +PUFA (MLP), and modified layer +PUFA+ antioxidants (MLPA) diet. At 36 weeks of age, six cockerels with ≥ 75 % sperm motility rate following analysis by Computer Aided Sperm Analysis (CASA) were selected from the base population in each dietary treatment group. Semen volume, sperm cells concentration and dead cells did not differ significantly among any of the groups. However, live and normal spermatozoa were significantly higher in the MLPA group. The bent mid-piece, bent mid-piece plus ...
Archives of Animal Nutrition, 2003
Three hundred and twenty 30-week old White Leghorn cockerels were housed in individual cages and distributed in a completely randomized factorial design of 5 6 3, with five oil sources (sunflower, soybean, canola, linseed and fish/soybean) and three levels of antioxidant (30, 200 and 400 mg of vitamin E/kg). The aim of this study was to evaluate the effect of the ratio of o6 : o3 fatty acids by the inclusion of different oil sources and of dietary supplementation with vitamin E on the reproductive performance of cockerels. The use of the fish/soybean combination determined the lowest total antioxidant status of the semen. However, the addition of vitamin E to the fish/soybean-oil-based diet resulted in a linear increase in semen volume, motility and sperm vigour in the 38th week and again in the 52nd week for motility and for sperm vigour and fertility rate in the periods from 50-53 and 41-53 weeks of age. The use of canola oil in the diet resulted in the highest fertility rate during 50-53 and 41-53 weeks of life. Animals receiving the soybean oil based diet showed the smallest fertility rate in the range from 50-53 weeks of age and concomitantly the highest level of cholesterol in the spermatozoa in the range from 47-51 weeks. An interaction between the vitamin E level and soybean oil was verified by the linear increase in motility and sperm vigour at 38 weeks of age. Later, the contrary was shown by a linear reduction in fertility in the periods from 44-46, 47-49 and 41-53 weeks of age. Cockerels that had been fed on the sunflower-oil-based diet showed the highest content of saturated fatty acids in the spermatozoa from 48-51 weeks. An interaction effect was observed between the vitamin E level and sunflower oil shown by a linear increase in the content of saturated fatty acids in the spermatozoa and a linear reduction in the C18 : 1o9, C18 : 2o6 and PUFA (C18 : 2o6 + C20 : 4o6) contents in the spermatozoa at 48-51 weeks and in sperm volume at 52 weeks of age.
Archiv für Tierernährung, 2003
Three hundred and twenty 30-week old White Leghorn cockerels were housed in individual cages and distributed in a completely randomized factorial design of 5 x 3, with five oil sources (sunflower, soybean, canola, linseed and fish/soybean) and three levels of antioxidant (30, 200 and 400 mg of vitamin E/kg). The aim of this study was to evaluate the effect of the ratio of omega6: omega3 fatty acids by the inclusion of different oil sources and of dietary supplementation with vitamin E on the reproductive performance of cockerels. The use of the fish/soybean combination determined the lowest total antioxidant status of the semen. However, the addition of vitamin E to the fish/soybean-oil-based diet resulted in a linear increase in semen volume, motility and sperm vigour in the 38th week and again in the 52nd week for motility and for sperm vigour and fertility rate in the periods from 50-53 and 41-53 weeks of age. The use of canola oil in the diet resulted in the highest fertility ra...
Dietary fish and evening primrose oil with vitamin E effects on semen variables in cockerels
British Poultry Science, 2005
1. Our aim was to determine the effect of n-3 (2%, wt/wt, fish oil rich diet) and n-6 (2%, wt/wt, evening primrose oil rich diet) fatty acid dietary supplementation and their combination with two concentrations of vitamin E (40 vs 200 mg/kg) on semen variables and on fatty acid and vitamin E profiles of spermatozoa in broiler breeders at 32, 42 and 52 weeks of age. 2. The inclusion of fish oil in the cockerel diets increased the docosahexaenoic acid proportion in the sperm phospholipid fraction, which was almost threefold higher compared to the other two groups irrespective of vitamin E supplementation. 3. In contrast, an increase in the proportion of total n-6 polyunsaturates, mainly 22:4n-6, was observed in the evening primrose oil group compared to the control only when the dietary content of vitamin E was increased to 200 mg/kg. 4. Sperm concentration was decreased in the fish and evening primrose oil groups if vitamin E was 40 mg/kg, but such an effect was prevented in the fish, not the evening primrose oil group, by increasing the vitamin E to 200 mg. 5. The proportion of motile spermatozoa was improved by the increased supplementation of vitamin E in all oil treatments.
The effect of enriched diets on Solea senegalensis sperm quality
Aquaculture, 2015
The development of specific broodstock diets could represent an important step toward the obtainment of good quality spawns in the Solea senegalensis. Thus, in the present work we tested the effects of two experimental diets in S. senegalensis males reproductive performance. One broodstock was fed with an experimental diet (Exp1) that, among other components, had a higher docosahexaenoic acid (DHA) content to improve sperm plasma membrane resistance. A second broodstock was fed with the other experimental diet (Exp2), similar to Exp1, but with vitamin E (vitE) and selenium (Se) to control for the increase in the lipid peroxidation caused by the higher proportion of unsaturated fatty acids. In addition, a control broodstock was fed with a diet resembling standard commercial broodstock diets. During the spawning season fish were sampled for blood and semen. Glutathione peroxidase activity, measured as an indirect contribution of Se, was significantly higher in blood plasma of males fed on Exp2 diet. There was a significant improvement in the sperm motility data in the broodstock fed on the Exp2 diet comparatively with the control. Both percentage of progressive spermatozoa and sperm velocity, usually positively related with fertilization rates, were significantly higher in fish fed on Exp2 diet, whereas fish fed on Exp1 did not show any improvement. Sperm plasma membrane integrity before and after motility activation was not affected by the experimental diets. Conversely, lipid peroxidation values (malondialdehyde determination) were significantly higher in sperm of males fed on Exp1 diet. This value was positively correlated with the amount of lysophosphatidyl-choline and sphingomyelin determined by HPLC. There was an increase in cholesterol and in several unsaturated fatty acids in sperm from males fed on Exp2 diet; at the same time, there was a decrease in the amounts of saturated fatty acids, both factors normally associated with improved male reproductive performance. The addition of some PUFA (such as DHA) and antioxidants in Exp2 diet and their synergic effects on the sperm membranes lipidic composition seem to be related with the improved S. senegalensis sperm quality. This study represents a clear step for the design of specific S. senegalensis broodstock diets and the development of this species farming.
Annals of Animal Science
This study aimed to investigate the effect of dietary n-3 fatty acids and ginger (Zingiber officinale) supplementation on semen quality, sperm fatty acids, and reproductive performance of roosters. Seventy-two roosters (30 weeks old) were randomly allocated into 4 dietary treatments including 1) basal diet as the control group (F0G0), 2) basal diet supplemented with 20 g/kg fish oil (F2G0), 3) basal diet supplemented with 30 g/kg ginger powder (PG) (F0G3), and 4) basal diet supplemented with 20 g/kg fish oil and 30 g/kg ginger powder (F2G3) for 10 consecutive weeks. The levels of sperm linolenic acid, EPA, and DHA were higher in fish oil-fed roosters (P<0.05). A lower percentage of sperm linoleic and arachidonic acids were recorded in F2G0 and F2G3 groups (P<0.05). At 38 weeks of age, a higher sperm volume was found in F0G3 roosters than in the F0G0 and F2G0 groups. From week 34 to the end of the experiment, sperm viability, sperm abnormality, and sperm motility were significa...
American Journal of Animal and Veterinary Sciences
The bird is one of the most important sources of animal protein for people and smallholders in developing countries. However, it is also important to look at their reproductive activity to maintain a sustainable population. Omega-3, omega-6 fatty acid and synthetic cholesterol may improve reproductive characteristics in animals including quail. Several treatments can be used to improve the quality of reproduction of the quail. The research was conducted to know the effect of the use of omega-3, omega-6 fatty acid and synthetic cholesterol on sperm quality, testosterone level, the testes and the uropygium size of male quails (Coturnix coturnix japonica). This research used 175 male quails at 6-w old. These divided into seven feed treatment groups. These are; R-0 (control), R-1 (0.163 mg omega-3/100g body weight (BW), R-2 (0.362 mg omega-3/100g BW), R-3 (0.163 mg omega-6/100g BW), R-4 (0.326 mg omega-6/100g BW), R-5 (20 mg cholesterol/100g BW) and R-6 (40 mg cholesterol/100g BW). The treatments were given orally for three different periods, within ten days. The data on testes size, uropygium sizes, and the testosterone level were analyzed statistically. The sperm quality data (sperm motility, sperm morphology, sperm viability, and sperm concentration) were analyzed with the Duncan Multiple Range Test (DMRT). The usage of omega-3, omega-6 fatty acid with a double dose (0.326 mg) showed the best response, which shows a significant increase of the testes weight (2.64 g), testes circumference (94.70 cm), testes volume (2.00 ml) and uropygium weight (2.75 g). Also, there was a significant increase in the level of testosterone (4.92 ng/ml). The use of double dose of (0.326 mg) omega-3 and omega-6 fatty acid or 0.163 mg omega-3 and omega-6 gave a good quality of sperm, including improvements in sperm motility (78.30% and 78.30%), sperm viability (73.19% and 86.06%) and sperm concentration (250.00 × 10 9 /ml and 255.30 × 10 9 /ml).
Cell journal, 2014
The effects of dietary fish oil on semen quality and sperm fatty acid profiles during consumption of n-3 fatty acids as well as the persistency of fatty acids in ram's sperm after removing dietary oil from the diet were investigated. In this experimental study, we randomly assigned 9 Zandi rams to two groups (isoenergetic and isonitrogenous diets): control (CTR; n=5) and fish oil (FO; n=4) for 70 days with a constant level of vitamin E in both groups. Semen was collected at the first week and at the last week of the feeding period (phase 1). After the feeding period, all rams were fed a conventional diet and semen samples were collected one and two months after removal of FO (phase 2). The sperm parameters and fatty acid profiles were measured by computer assisted semen analyzer (CASA) and gas chromatography (GC), respectively. The completely randomized design was used and data were analyzed with SPSS version 16. Dietary FO had significant positive effects on all sperm quality a...
Cell Journal, 2013
Objective: The effects of dietary fish oil on semen quality and sperm fatty acid (FA) profiles during consumption of n-3 FA as well as the persistency of FA in ram's sperm after removing dietary oil from the diet were investigated. Materials and Methods: Nine Zandi's rams were randomly assigned to two groups (isoenergetic and isonitrogenous diets): Control (CTR; n=5) and Fish oil (FO; n=4) for 70 days with a constant level of vitamin E in both groups. Semen was collected at the first week and at the end week of feeding period (phase 1). After feeding period, all rams were fed a conventional diet and semen samples were collected one and two months after removing FO (phase 2). The sperm parameters and FA profiles were measured by computer assisted semen analyzer (CASA) and gas chromatography (GC), respectively. The completely randomized design was used and data were analyzed using the SPSS 16. Does the conventional diet contain any fats/lipids? If so, what is the concentration of these fats given to the control group compared to treatement group? No, it dosnt. This diet typically have 2.5-3 % ether extract or crude fat originated from forages and some garin such as barley. Results: Dietary FO had significant positive effects on all sperm quality and quantity parameters compared with CTR during feeding period (P<0.05). The positive effects of FO on sperm concentration and total sperm output were observed one and two months after removing FO (P<0.05), whereas, other sperm parameters were unaffected. Before feeding, C14 (Myristic acid), C16 (Palmitic acid), C18 (Stearic acid), C18:1 (Oleic acid) and C22:6 Cell Journal (Yakhteh