Biofilm formation, antimicrobial susceptibility and virulence genes of Uropathogenic Escherichia coli isolated from clinical isolates in Uganda (original) (raw)
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Journal of Evolution of Medical and Dental Sciences, 2012
BACKGROUND: Microorganisms growing in multilayered cell clusters embedded in a matrix of extracellular polysaccharide (slime) which facilitates the adherence of these microorganisms to biomedical surfaces and protect them from host immune system and antimicrobial therapy. There are various methods to detect biofilm production like Tissue Culture Plate (TCP) ,Tube method (TM) ,Modified Congo Red Agar Method (MCRA),bio luminescent assay ,piezoelectric sensors and fluorescent microscopic examination. OBJECTIVES : This study was conducted to compare three methods for the detection of biofilms and compare with antibiotic sensitivity pattern, in uropathogenic Escherichia coli. METHOD: This study was carried out at the Department of Microbiology Dr. B. R. Ambedkar Medical College from Dec 2011 to June 2012. Total number of 107 clinical Escherichia coli isolates were randomly selected from all age groups were subjected to biofilm detection methods and their antibiotic resistance pattern was compared. Isolates were identified by standard phenotypic methods. Biofilm detection was tested by TCP, TM and MCRA methods. Antibiotic susceptibility test of uropathogenic E coli was performed using Kirby-Bauer disc diffusion method according to CLSI guidelines. RESULTS: From the total of 107 clinical isolate 74 (69.1 %) isolates showed biofilm formation by all the TCP, TM, CRP methods. Biofilm forming isolates from catheter associated UTI showed drug resistance to more than 6 drugs. Only 2(13.3%) isolates from Asymptomatic UTI showed biofilm by TM & MCRA methods & were sensitive all drugs. Biofilm forming isolates from symptomatic UTI showed mixed drug resistance pattern. CONCLUSION: We conclude from our study that biofilm formation is more common in catheterized patients. TCP method is more quantitative and reliable method for the detection of biofilm forming microorganisms as compared to TM and MCRA methods. So TCP method can be recommended for screening of biofilm as virulence marker in drug resistant E coli isolates.
Jundishapur Journal of Microbiology
Background: Uropathogenic Escherichia coli (UPEC) strains, encoding superficial and secretory virulence factors, can lead to colonization and facilitation of bacterial growth in the host urinary tract, causing Urinary Tract Infection (UTI). Objectives: This study determined the ability of biofilm formation by the Congo red agar (CRA) method, the presence of virulence genes using the multiplex polymerase chain reaction (PCR) method, and the relationship between biofilm formation and antibiotic resistance patterns and virulence genes in E. coli clinical isolates in Yasuj. Methods: This cross-sectional study was performed on 144 UPEC isolates collected in 2017. Biofilm formation was detected by the CRA phenotypic assay and virulence factors by the multiplex PCR method. Antibiotic resistance tests were performed by the Kirby-Bauer method. Results: Out of 144 isolates of E. coli, 22 (19.4%) isolates showed to be strong biofilm producers, 27 (23.8%) moderate biofilm producers, and 64 (56....
Journal of Laboratory Physicians
BACKGROUND: Escherichia coli accounts for 70%–95% of urinary tract infections (UTIs). UTI is a serious health problem with respect to antibiotic resistance and biofilms formation being the prime cause for the antibiotic resistance. Biofilm can restrict the diffusion of substances and binding of antimicrobials. In this context, the present study is aimed to perform in vitro detection of biofilm formation among E. coli strains isolated from urine and to correlate their susceptibility pattern with biofilm formation. MATERIALS AND METHODS: A total of 100 E. coli strains isolated from patients suffering from UTI were included in the study. The identification of E. coli was performed by colony morphology, Gram staining, and standard biochemical tests. The detection of biofilm was carried out by Congo Red Agar (CRA) method, tube method (TM), and tissue culture plate (TCP) method. Antimicrobial sensitivity testing was performed by Kirby–Bauer disc diffusion method on Muller–Hinton agar plat...
Polish Journal of Microbiology
To explore the prevalence of multidrug-resistant community-associated uropathogenic Escherichia coli (UPEC) and their virulence factors in Western Saudi Arabia. A total of 1,000 urine samples were examined for the presence of E. coli by selective plating on MacConkey, CLED, and sheep blood agar. Antimicrobial susceptibility patterns were determined using Vitek® 2 Compact (MIC) and the disc diffusion method with Mueller-Hinton agar. Genes encoding virulence factors (kpsMTII, traT, sat, csgA, vat, and iutA) were detected by PCR. The overall prevalence of UTI-associated E. coli was low, and a higher prevalence was detected in samples of female origin. Many of the isolates exhibited resistance to norfloxacin, and 60% of the isolates showed resistance to ampicillin. No resistance to imipenem, meropenem, or ertapenem was detected. In general, half of the isolates showed multiple resistance patterns. UPEC exhibited a weak ability to form biofilms, where no correlation was observed between ...
Antimicrobial Resistance & Infection Control, 2016
Background: Uropathogenic Escherichia coli O-Serogroups with their virulence factors are the most prevalent causes of UTIs. The present research performed to track common uropathogenic E.coli serogroups, antibiotic resistance pattern of strains and prevalence of virulence genes in isolations having the ability to constitute biofilm. Methods: In this research 130 E.coli isolation from patients having UTI symptoms were collected and antimicrobial resistance pattern was performed by Kirby-Bauer method. Polymerase chain reaction was done using primer pairs to identify common serogroups of uropathogenic E.coli and studying virulence genes in isolations creating biofilm. Results: Among 130 E.coli isolates, 80 (61.53 %) were able to make biofilm that 15 isolates (18.75 %) indicated strong reaction, 20 (25 %) of medium and 45 (56.25 %) of weak biofilm reaction. Among isolations creating biofilm, the highest resistance reported to Ampicillin (87.5 %) and the lowest to Nitrofurantoin (3.75 %). The frequency of fimH, pap, sfa and afa genes in isolations having the ability to create strong biofilm reported 93.33 %, 86.66 %, 86.66 % and 66.66 %, respectively. Conclusions: The findings indicated the importance of virulence genes in serogroups producing uropathogenic E.coli biofilm. It is recommended that strains producing biofilm before antibiotic use should be studied.
Diseases
Urinary tract infections (UTIs) are among the most common infections requiring medical attention worldwide. The production of biofilms is an important step in UTIs, not only from a mechanistic point of view, but this may also confer additional resistance, distinct from other aspects of multidrug resistance (MDR). A total of two hundred and fifty (n = 250) Escherichia coli isolates, originating from clean-catch urine samples, were included in this study. The isolates were classified into five groups: wild-type, ciprofloxacin-resistant, fosfomycin-resistant, trimethoprim-sulfamethoxazole-resistant and extended spectrum β-lactamase (ESBL)-producing strains. The bacterial specimens were cultured using eosine methylene blue agar and the colony morphology of isolates were recorded. Antimicrobial susceptibility testing was performed using the Kirby–Bauer disk diffusion method and E-tests. Biofilm-formation of the isolates was carried out with the crystal violet tube-adherence method. n = 7...
GMS hygiene and infection control, 2015
The Escherichia coli (E. coli) bacterium is one of the main causative agents of urinary tract infections (UTI) worldwide. The ability of this bacterium to form biofilms on medical devices such as catheters plays an important role in the development of UTI. The aim of the present study was to investigate the possible relationship between virulence factors and biofilm formation of E. coli isolates responsible for urinary tract infection. A total of 100 E. coli isolates isolated from patients with UTI were collected and characterized by routine bacteriological methods. In vitro biofilm formation by these isolates was determined using the 96-well microtiter-plate test, and the presence of fimA, papC, and hly virulence genes was examined by PCR assay. Data analysis was performed using SPSS 16.0 software. From 100 E. coli isolates isolated from UTIs, 92% were shown to be biofilm positive. The genes papC, fimA, and hly were detected in 43%, 94% and 26% of isolates, respectively. Biofilm fo...
Jundishapur Journal of Microbiology, 2014
Background: The emergence of antimicrobial resistant strains of Escherichia coli has raised considerable interest in understanding the diversity and epidemiology of E. coli infections in humans. Virulence factors of E. coli determine the specific infections caused by this microorganism. Objectives: This study aimed to determine the prevalence of eight E. coli virulence factors and their association with antimicrobial resistance in bacteria isolated from patients with urinary tract infections (UTI). Patients and Methods: One thousand patients with UTI were enrolled in this cross-sectional study. Antimicrobial susceptibility was examined by disc diffusion method according to CLSI guidelines. After DNA extraction, the materials were probed by PCR for eight virulence factors genes, namely fimH, hly, iucC, ibeA, sfa/foc, neuC, papC, and afa genes.
Purpose: Escherichia coli strains are the most frequent cause of urinary tract infections. Biofilm formation allows the strains to persist a long time in the genitourinary tract and interfere with bacterial eradication. We determined the possible relationships between the different urinary tract infections, and in vitro biofilm formation, the presence of urovirulence factors and nalidixic acid resistance. Materials and Methods: A total of 151 E. coli strains collected from patients with cystitis (44 strains), pyelonephritis and prostatitis (32) were analyzed for in vitro biofilm formation, the phylogenetic group, the presence of several urovirulence factors and resistance to nalidixic acid. Results: E. coli strains causing prostatitis produced biofilm in vitro more frequently than those causing other urinary tract infections and had a higher frequency of hemolysin (p ϭ 0.03 and 0.0002, respectively). However, only hemolysin was independently associated with prostatitis. On the other hand, strains forming biofilm presented a significantly higher frequency of hemolysin and type 1 fimbriae expression. Conclusions: Although hemolysin is the main virulence factor by which E. coli causes acute prostatitis, the association between hemolysin and biofilm formation may result in increased ability of E. coli strains to persist in the prostate.
Journal of Evidence Based Medicine and Healthcare, 2021
BACKGROUND Urinary tract infections are some of the most common community-acquired as well as nosocomial infections with E. coli being the most common pathogen. There is increased antimicrobial resistance among bacteria worldwide. One of the important mechanisms of resistance and virulence of bacteria is biofilm formation. This study was conducted to find out the association between antibiotic resistance pattern and biofilm formation in E. coli in non-catheterised patients of UTI in a tertiary care hospital. We further wanted to determine the association between the ability of E. coli to form biofilm and their ability to produce extended-spectrum beta-lactamases (ESBLs) and carbapenemase in non-catheterised patients. METHODS Urine samples collected from 300 non-catheterised patients who had symptoms of UTI were inoculated into MacConkey’s agar and blood agar media. Then identification and antibiotic susceptibility tests were done. Phenotypic detection of ESBL production was done by ...