Modeling of growth and energy metabolism of pichia pastoris producing a fusion protein (original) (raw)

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Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in< i> Pichia pastoris</i> fed-batch cultures Cover Page

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Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures Cover Page

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Characterization of methanol utilization negative Pichia pastoris for secreted protein production: New cultivation strategies for current and future applications Cover Page

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Fermentation and Oxygen Transfer Characteristics In Recombinant Human Growth Hormone Production by Pichia Pastoris In Sorbitol Batch and Methanol Fed-Batch … Cover Page

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Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter Cover Page

Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement

Journal of industrial microbiology & biotechnology, 2003

Fed-batch fermentation of a methanol utilization plus (Mut(+)) Pichia pastoris strain typically has a growth phase followed by a production phase (induction phase). In the growth phase glycerol is usually used as carbon for cell growth while in the production phase methanol serves as both inducer and carbon source for recombinant protein expression. Some researchers employed a mixed glycerol-methanol feeding strategy during the induction phase to improve production, but growth kinetics on glycerol and methanol and the interaction between them were not reported. The objective of this paper is to optimize the mixed feeding strategy based on growth kinetic studies using a Mut(+) Pichia strain, which expresses the heavy-chain fragment C of botulinum neurotoxin serotype C [BoNT/C(Hc)] intracellularly, as a model system. Growth models on glycerol and methanol that describe the relationship between specific growth rate ( micro ) and specific glycerol/methanol consumption rate ( nu(gly), nu...

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Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement Cover Page

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Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris Cover Page

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A rational approach to improving productivity in recombinantPichia pastoris fermentation Cover Page

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A quantitative study of methanol/sorbitol co-feeding process of a Pichia pastoris Mut+/pAOX1-lacZ strain Cover Page

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Enhancement of thermostable β-glucosidase production in a slow methanol utilization strain of Pichia pastoris by optimization of the specific methanol supply rate Cover Page