The Comparison of Extracts (Ethanol And Aquos Solvents) Muntingia calabura Leaves on Total Phenol, Flavonid And Antioxidant (Ic50) Properties (original) (raw)
Related papers
Quantitative and Qualitative Phytochemicals Analysis of Muntingia calabura
Journal of Biology Agriculture and Healthcare, 2014
Muntingia calabura is widely cultivated and has become one of the common roadside trees in Indonesia. The present study investigates the qualitative and quantitative analysis of the major bioactive constituents of plant Muntingia calabura in some solvents (water, methanol, ethanol, chloroform, ether, and citrid acid). Phytochemical screening method was used to identify qualitative analysis of bioactive component. While the quantitative analysis was analysed by UHLPC. The results showed the highest percentage extract concentration was resulted by methanol followed by water, ethanol, chloroform, ether and citric acid 13.15%, 11.93%, 9.57%, 9.04%, 4.52% and 3.69% respectively. The bioactive component ie., saponins, flavonoids and tannin were found in all those solvent. The higher concentration of flavonoid, saponin and tannin were resulted from polar solvent ie., water, methanol and ethanol. While non polar solvent has lower in concentration. Epigallocatechin Gallate (EGCG) and Genistein content of extract Muntingia calabura from methanol solvent were 135.15 µg/g and 136.29 µg/g respectively.
Phytochemical Screening of Muntingia Calabura Fruit for Antioxidant and Cytotoxic Activities
Journal of Advanced Research in Applied Sciences and Engineering Technology
Muntingia calabura (M. calabura) is an indigenous fruit species that is widely distributed in Malaysia and traditionally believed for constituting medicinal properties, yet little-known and underutilized. Hence, this study aimed to perform an extraction of M. calabura fruit to identify phytochemicals potential by carrying out several analysis such as Total Phenolic Content, Fourier Transform Infrared Spectrum (FTIR), High Performance Liquid Chromatography (HPLC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Besides, the antioxidant and cytotoxicity properties of M. calabura were also identified from Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and brine shrimp lethality tests. The finding suggested that further study on the modification of M. calabura extraction should be carried out to explore maximal potential of M. calabura as natural medicine and commercial use .
International Journal of Pharmaceutical Sciences Review and Research, 2021
The present study was carried out to determine the possible LC-MS and anti-microbial activity of plant extracts prepared from Muntingia calabura and phytochemical screening was demonstrated. The extracts from the plant parts like leaf were prepared in different solvents like petroleum ether, chloroform and ethanol. The highest antimicrobial potentials were observed for the ethanolic extracts against (Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida albicans) Its efficacy was comparable to the standard drug, amikacin. Significant amount of tannins, alkaloids, steroids and flavonoids were found. The present study provides evidence that solvent extract of M. calabura contains medicinally important bioactive compounds and this justifies the use of plant species as traditional medicine for treatment of various diseases. Likewise, the leaf extract of Muntingia calabura showed more zone of inhibition with fungus Candida albicans. This shows i...
Open Access Macedonian Journal of Medical Sciences, 2022
BACKGROUND: Seri (Muntingia calabura L.) leaves are a plant that is often found and have not been used in various treatments even though it is reported to have various groups of bioactive compounds such as phenolic, flavonoids, tannins, saponins, steroids, and triterpenoids. AIM: This study aimed to determine the total phenolic content, antioxidant activity and identify the content of potential bioactive compounds contained in the ethyl acetate fraction from M. calabura leaves. METHODS: M. calabura L. leaves fraction was carried out by maceration method using ethanol followed by partition starting with n-hexane, chloroform, and finally ethyl acetate as solvent. The ethyl acetate fraction was continued for phytochemical screening for the content of bioactive compounds using standard reagents, determination of total phenol content by colorimetric method, determination of antioxidant activity using the DPPH method, and analysis of bioactive compounds using gas chromatography–mass spect...
Context: Different parts of Muntingia calabura L. (Elaeocarpaceae), or 'kerukup siam' in Malay, have been reported to possess medicinal values, supported by a number of scientific studies. Objective: To gather all information related to the ethnomedicinal uses, phytochemical compositions, and pharmacological activities of M. calabura and present them as a comprehensive and systematic review article. Materials and methods: The literature has been retrieved from a number of databases (e.g. Pubmed, Science Direct, Springer Link. etc.). General web searches were also carried out using Google and Yahoo search engines by applying some related search terms (e.g. Muntingia calabura, phytochemical, pharmacological, extract, and traditional uses). The articles related to agriculture, ecology, and synthetic works and those using languages other than English or Malay have been excluded. The bibliographies of papers relating to the review subject were also searched for further relevant references. Results and discussion: The literature searched conducted using the abovementioned internet search engines only lead to the identification of 36 journals published as early as 1987. From the articles reviewed, M. calabura possessed various pharmacological activities (e.g. cytotoxic, antinociceptive, antiulcer, anti-inflammatory etc.), which supported the folklore claims and could be attributed to its phytoconstituents.
Journal of Herbmed Pharmacology
Introduction: Muntingia calabura is used for many medicinal advantages. So far, limited study has been done for the bioactivities of M.calabura fruit. The study aimed to investigate the enzyme inhibitory, antioxidant, and antibacterial activities of M.calabura fruit. Methods: Ethanol extract of M.calabura fruit was tested for its inhibitory enzyme activities against key enzymes linked to human pathologies, such as diabetes (α-glucosidase and α-amylase), hyperuricemia (xanthine oxidase), and obesity (lipase). The antioxidant properties were investigated using different in vitro assays (DPPH, CUPRAC, reducing power, phosphomolybdenum, metal chelating and DNA-Damage protection assays). The fruit was also evaluated for its antibacterial activity against several gram positive and negative bacteria. Results: The total phenolic and flavonoid contents of the extract were 10.85 mgGAE/g and 3.30 mg QE/g, respectively. The fruit extract showed good inhibition against α-glucosidase and α-amylas...
Natural Resources for Human Health
The study aimed to identify bioactive compounds in Muntingia calabura leaf and root methanolic extracts. The Gas Chromatography and Mass Spectroscopy (GC-MS) technique were used to identify bioactive compounds. GC-MS analysis revealed 38 compounds in the leaf and 15 compounds in the root methanolic extracts of M. calabura. The prime potent compound found in leaf extract is 2-{3-[(E)-2-(1H-indol-3-yl)ethenyl]-1,2,4-oxadiazol-5-yl}phenol with 5.78% peak area and cholest-4-en-6-on-3-ol is found in root extracts, has the highest 63.7% peak area and another potent compound Lupeol has 7.3% peak area. The bioactive compounds identified in M. calabura have antibacterial activity against various bacterial strains such as gram-positive and gram-negative bacteria, which showed the efficacy of in vivo plant extracts. These findings validate the therapeutic potentiality of M. calabura leaf and root samples. Furthermore, these screened potential bioactive compounds can be used effectively for bio...
Journal of Fundamental and Applied Pharmaceutical Science, 2022
Muntingia calabura is one of the plants employed to produce herbal-based treatments. Muntingia calabura leaves are traditionally used as an alternative medicine due to their secondary metabolites. The maceration method extracted Muntingia calabura leaves using 96% ethanol solvent for 3 x 24 hours. The fractionation process was carried out using a separating funnel method with different polarities, such as n-hexane, ethyl acetate, and ethanol-water. Thin-layer chromatography (TLC) was used to confirm the phytochemical screening. TLC conditions under UV light 254 and 366 nm using solvents, such as chloroform: methanol (alkaloids), butanol: acetic acid: water (flavonoids), chloroform:methanol: water (saponins), and chloroform: methanol (phenolic). The phytochemical screening results of extracts and Muntingia calabura fractions contained secondary metabolites, such as alkaloids, flavonoids, tannins, saponins, and phenolics. TLC results showed that n-hexane fraction contained flavonoid a...
Identification of Active Compounds on Muntingia calabura L.Leaves using Different Polarity Solvents
Indonesian Journal of Chemical Science and Technology (IJCST), 2020
Plant of Muntingia calabura L are often known as “kersen”, "seri or "cherry". Leaves of Muntingia calabura L. contains many benefits but its properties are still little known to the public. It contains secondary metabolites which have many uses. This study was aimed to determine the content of secondary metabolites in this leaf. Leaves extracts were obtained by maceration extraction for 3 times 24 hours using polar, semi-polar and non-polar solvents to determine the solubility of secondary metabolite compounds in each solvent. The solvents used were ethanol, ethyl acetate and n-hexane. The leaveswere dissolved a lot in polar solvents, marked by the formation of a dark green color in ethanol-series extracts, the color fades more in semi-polar and non-polar solvents. The three leaves extracts were tested for secondary metabolite contents by phytochemical screening tests. Phytochemical screening was an initial selection stage to detect classes of chemical compounds conta...