Quantitative trait loci and differential gene expression analyses reveal the genetic basis for negatively associated β-carotene and starch content in hexaploid sweetpotato [Ipomoea batatas (L.) Lam.] (original) (raw)
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Molecular Breeding, 2011
Development of orange-fleshed sweetpotatoes (OFSP) is desired for the improvement of the food supply and nutritional status of millions of people in developing countries, particularly in sub-Saharan Africa. However, sweetpotato [Ipomoea batatas (L.) Lam] breeding is challenging due to its genetic complexity, and marker-assisted breeding tools are needed to facilitate crop improvement. We identified quantitative trait loci (QTL) for dry-matter, starch, and β-carotene content in a hexaploid sweetpotato mapping population derived from a cross between Tanzania, a white-fleshed, high dry-matter African landrace, and Beauregard, an orange-fleshed, low dry-matter sweetpotato cultivar popular in the USA. Two parental maps were constructed using a population of 240 clones. Strong correlations were observed between starch and dry-matter content (r > 0.8, P r = –0.6, P
Genetic Control of Beta-carotene, Iron and Zinc Content in Sweetpotato
Journal of Plant Studies, 2016
Micronutrients deficiency is a major contributor to poor health in developing countries. It can be alleviated by biofortification or enrichment of staple crops with micronutrients. Sweetpotato is a major staple crop in numerous tropical countries and is naturally biofortified. In spite of extensive promotion of orange-fleshed sweetpotatovarieties (OFSPs), they are poorly utilized as staple food in most parts of West Africa because of their low dry matter and high sugar content. Beta-carotene is positively correlated with iron and zinc content in sweetpotato. Development of sweetpotato cultivars with end-user preferred traits and higher content of beta-carotene, iron and zinc will alleviate their deficiencies. Knowledge on the genetic control of these traits is critical for their improvement in sweetpotato. This study used diallel mating design to estimate general combining ability (GCA) and specific combining ability (SCA) of storage root beta-carotene, iron and zinc content to dete...
QTL analysis and GWAS of agronomic traits in sweetpotato (Ipomoea batatas L.) using genome wide SNPs
Breeding Science
While sweetpotato (Ipomoea batatas L.) improvement has generally been done by field-based selection, molecular genetic studies on traits of interest, i.e., molecular markers are needed for enhancing the breeding program of this world's 7 th most important crop, as such markers facilitate marker-assisted selection. Here, we performed a combined approach of QTLs analyses and GWAS of storage root β-carotene content (BC), dry-matter (DM) and starch content (SC) using the genetic linkage maps constructed with 5,952 and 5,640 SNPs obtained from F 1 progenies between cultivars 'J-Red' and 'Choshu'. BC was negatively correlated with DM (r =-0.45) and SC (r =-0.51), while DM was positively correlated with SC (r = 0.94). In both parental maps, a total of five, two and five QTL regions on linkage groups 7 and 8 were associated with BC, DM and SC, respectively. In GWAS of BC, one strong signal (P = 1.04 × 10-9) was observed on linkage group 8, which co-located with one of the above QTL regions. The SNPs markers found here, particularly for βcarotene, would be useful base resources for future marker-assisted selection program with this trait.
Breeding Science
Molecular markers are needed for enhancing the development of elite sweetpotato (Ipomoea batatas (L.) Lam) cultivars with a wide range of commercially important traits in sub-Saharan Africa. This study was conducted to estimate the heritability and determine trait correlations of storage root yield, dry matter, starch and βcarotene content in a cross between 'New Kawogo' × 'Beauregard'. The study was also conducted to identify simple sequence repeat (SSR) markers associated with these traits. A total of 287 progeny and the parents were evaluated for two seasons at three sites in Uganda and genotyped with 250 SSR markers. Broad sense heritability (H 2) for storage root yield, dry matter, starch and β-carotene content were 0.24, 0.68, 0.70 and 0.90, respectively. Storage root β-carotene content was negatively correlated with dry matter (r =-0.59, P < 0.001) and starch (r =-0.93, P < 0.001) content, while storage root yield was positively correlated with dry matter (r = 0.57, P = 0.029) and starch (r = 0.41, P = 0.008) content. Through logistic regression, a total of 12, 4, 6 and 8 SSR markers were associated with storage root yield, dry matter, starch and β-carotene content, respectively. The SSR markers used in this study may be useful for quantitative trait loci analysis and selection for these traits in future.
Genetic analysis of yield and flesh colour in sweetpotato
African Crop Science Journal, 2016
Pre-breeding information on the inheritance mechanism of important sweetpotato (Ipomoea batatas L.) agronomic traits is still limited. This study aimed at assessing the inheritance of five sweetpotato agronomic traits, viz. marketable fresh root yield (MFRY) and number (MNR), total fresh root yield (TFRY) and number (TNR) and root β-carotene content (RBCC). A 5 x 5 full diallel was performed and F 1 progenies, evaluated in two environments alongside the parents. The data were subjected to ANOVA and DiallelSAS-05 Griffing's method 1. Simple Sequence Repeat (SSR) based genetic distance and cluster analysis were performed on the parental lines using Jaccard's coefficient and the unweighted pair group method with arithmetic averages (UPGMA). Significant differences (P<0.01) were detected among the genotypes for MFRY, MNR, TFRY, TNR and RBCC. Significant general and specific combining ability (P<0.01) effects were observed for all five traits. Additive gene action was predominantly involved in the inheritance of these traits. High broad sense heritability values were observed for the four yield parameters and for RBCC. The Jaccard's similarity coefficient indicated moderate to low genetic similarity distances among the parents, implying high diversity. The knowledge on the inheritance and diversity of the parental genotypes enables more effective choice of parents in breeding improved varieties.
International Journal of Current Microbiology and Applied Sciences, 2021
Sweet potato (Ipomoea batatas (L.) Lam) is a vegetatively propagated, highly heterozygous and outcrossing dicotyledonous crop (Woolfe, 1992). Over 80% of global sweet potato production occurs in the developing world, particularly in Africa and Asia. The importance of sweet potato as a food, feed, and nutrition and income security crop is widely recognized (FAOSTAT, 2017). Sweet potato breeding has relied on the ability of breeders to identify parental genotypes possessing desirable traits and to combine these parents through hybridization schemes such as the polycross and controlled cross International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 10 Number 01 (2021) Journal homepage: http://www.ijcmas.com
Genetic control of dry matter, starch and sugar content in sweetpotato
Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, 2016
Sweetpotato (Ipomoea batatas L. (Lam)) is a nutritious food security crop for most tropical households, but its utilisation is very low in Ghana compared to the other root and tuber crops due to lack of end-user-preferred cultivars. Knowledge on the genetic control of important traits such as dry matter, starch and sugar content of sweetpotato storage roots in a breeding population is critical for making breeding progress in developing sweetpotato varieties preferred by farmers and consumers. This study used diallel mating design to elucidate general combining ability and specific combining ability, to determine the gene action controlling storage root dry matter, starch and sugar content in sweetpotato and the heterotic potential of the traits to facilitate the crop's improvement for increased utilisation. A general model for estimating genetic effects, GEAN II, was used to analyse the data. Genetic variability was seen for dry matter, starch and sugar content of sweetpotato and much of this genetic variation was additive in nature. The study also revealed significant heterosis in sweetpotato which offers opportunity for breeding non-sweet, high dry matter sweetpotato varieties that are preferred by farmers and consumers in Ghana.
HortScience, 2018
Orange-fleshed sweetpotato (OFSP) is an effective, low-priced, and sustainable source of β-carotene (provitamin A). However, most OFSP varieties have low storage root dry matter content (DMC), which influences their acceptance by small-scale farmers and hence needs to be improved. The objective of the study was to determine the combining ability, type of gene action, heterosis and heritability of storage root DMC, β-carotene content, and yield-related traits of selected sweetpotato clones for further evaluation and breeding. Crosses were conducted using a 7 × 7 half-diallel mating design and a total of 28 genotypes (seven parents and 21 crosses) were evaluated at four locations in Ethiopia using a 7 × 4 alpha lattice design with two replications. The performance of the genotypes was significantly different (P < 0.01) across the four locations for storage root DMC, β-carotene content, sweetpotato virus disease (SPVD) reaction, storage root yield, and harvest index (HI). The genera...