Plant Genetics @Bullet Original Paper (original) (raw)

Journal of applied genetics

Abstract

Comparative functional analysis of two wheat Na + /H + antiporter SOS1 promoters in Arabidopsis thaliana under various stress conditions Abstract The bread wheat TaSOS1 has been previously shown to be induced by salt stress treatment. To further investigate the regulation of the TaSOS1 gene, the two geno-mic fragments Pr SOS1-AB and Pr SOS1-D have been isolated and sequenced. Pr SOS1-AB and Pr SOS1-D are the promoter regions of SOS1 alleles, which are localised on genomes A and/or B, and on genome D, respectively. Sequence analysis of these two promoters revealed the presence of cis-regulatory elements which could be required for abiotic stress and abscisic acid (ABA) responsiveness. Histochemical assays of stably trans-formed Arabidopsis T3 plants showed that Pr SOS1-AB and Pr SOS1-D are active in this heterologous system, and their activities were almost the same at early developmental stages (4-, 8-and 12-day-old transgenic Arabidopsis seedlings). Nevertheless, β-glucuronidase (G...

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