Production and performance of larvae and spat of pure and hybrid species of Mytilus chilensis and M. galloprovincialis from laboratory crosses (original) (raw)
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Gayana
Erroneous identifi cation of the mussel, Mytilus galloprovincialis (Lamarck 1819) as the specie, Mytilus chilensis (Hupe 1854) in the Bay of Concepcion, Chile Identifi cación errónea del mejillón, Mytilus galloprovincialis (Lamarck 1819) como la especie, Mytilus chilensis (Hupe 1854) en la Bahía de Concepción, Chile ABSTRACT This communication informs that presence of the Chilean mussel, Mytilus chilensis (Hupe 1854) in the coast of the Bay of Concepcion (Chile) reported in the scientifi c literature is an erroneous identifi cation, being Mytilus galloprovincialis (Lamarck 1819) the right species.
Aquaculture, 2019
Choosing an alternative species of mussel for the aquaculture industries, will be possible if it has equal or greater yield than the mussel cultivated. This study compares the growth of the chilean native mussel Mytilus chilensis with the non-native Mediterranean mussel Mytilus galloprovincialis and evaluate if environmental variables and cultivation depth affects mussels growth. Mussel were seeded in summer, with the same mean total weight and shell length for both species. At the end of the experiment, shell length (SL), condition index and gonadosomatic index were compared over
Effect of temperature on survival, growth and development of Mytilus galloprovincialis larvae
Aquaculture Research, 2011
Mussel aquaculture is widely prevalent worldwide, but generally relies on natural seed collection, which does not always meet the needs of the producers. Thus, development of mussel hatcheries is of economic interest in some parts of the world, such as Europe; it provides opportunities not only on annual reliability of seed but also on genetic improvements. To broaden knowledge on mussel larval physiology, we carried out temperature treatments (17, 20 and 24 1C) on Mytilus galloprovincialis larvae under laboratory conditions. The trials ended when 30% of the larval population was in the post-larval stage. The temperature coe⁄cient Q 10 indicated a strong relationship between temperature and increase in growth from 17 to 20 1C, but not between 20 and 24 1C. Exposure of M. galloprovincialis larvae to 17 1C resulted in poor growth, low survival and a delayed development and was considered to be inadequate for M. galloprovincialis larval culture. Rearing the larvae at 20 or 24 1C produced better growth, higher survival rates and faster metamorphosis as compared with 17 1C. The temperature region within 20 and 24 1C was suggested as adequate for the mussel M. galloprovincialis larval culture, and implications of these results on the development of commercial hatcheries were discussed.
Ciencias Marinas, 2012
D-veliger larvae from Mytilus chilensis broodstocks from natural banks of Punta Arenas and Chiloé (Chile) were grown at 9 ± 0.5 ºC and 15 ± 0.5 ºC to compare results under two culture temperatures. During this experiment, larvae were fed Isochrysis galbana (clone T-ISO). The larvae were grouped into four groups depending on origin of broodstock and the culture temperature. The growth rate was statistically higher in the group from Punta Arenas at 15 ºC, while settlement length was smaller in the group from Chiloé at 9 ºC. In both cases, the remaining groups did not differ significantly. Settlement survival showed no significant differences between populations and temperatures. Both populations showed a better use of accumulated thermal units (ºC day-1) during growth at 9 ºC than at 15 ºC. Despite the genetic differentiation of the Punta Arenas population, the productive outcome of M. chilensis larvae from broodstocks from different latitudes is similar.
Genetic composition of Mytilus species in mussel populations from southern Chile
Mussels are one of the most cultivated and commercialized bivalves worldwide and in southern Chile its culture represent an important economic activity. The species identification within the Mytilus genera, by morphological features, is unreliable, so we used a polymorphism RFLP in the gene encoding the polyphenolic adhesive protein as a species-specific genetic marker to describe Mytilus species diversity in southern Chile, and evaluate possible applications in traceability, food quality and safety. Using Me 15-16 marker most mussels were M. chilensis, finding no other pure individuals; however, putative hybrids of M. chilensis x M. trossulus and M. chilensis x M. galloprovincialis were detected. There was no evidence of M. edulis. The presence of the M. trossulus allele, faraway from its distribution area, demands further analysis with different genetic markers to allow a better understanding of its origin. In addition, the correspondence between markers that distinguishes northern from southern hemisphere M. galloprovincialis, with those who discriminates between M. chilensis and M. galloprovincialis would contribute to the taxonomic status of Chilean blue mussels. In Chile, the genetic composition of Mytilus indicates that geographical origin of mussels and its traceability cannot be established merely from the identification of the species. The use of other markers would be required.
Aquaculture, 2009
The method currently used for accurate identification of mussel larvae is based on the study of morphological traits under an optical microscope, which is a tedious and time-consuming procedure. It also requires considerable taxonomic experience, because of the similarities in the larvae of different bivalves present in the plankton. The introduction of specific monoclonal antibodies (mAbs) directed against mussel larvae, such as M22.8 and M36.5 mAbs developed by our group, may allow an easier and more specific identification. Handling conditions and sample preservation were optimized for using these antibodies in the monitoring of mussel larvae in the Galician rías. Bivalve larvae can be isolated very efficiently from plankton samples by centrifugation in sugar solution. Samples can be maintained at 4°C on the boat and during transport to the laboratory, and then preserved for longer periods at − 80°C or in liquid nitrogen until staining. In an attempt to minimize the time required for immunodetection, different incubation periods were tested, which showed that only 5 min of incubation with the primary monoclonal antibody and 60 min with the secondary antibodies are sufficient to stain over 98% of the larvae. Here, we show that the use of mAbs allows a rapid and specific recognition of mussel larvae, with clear advantages over the traditional method, particularly for large-scale field studies.
Gayana (Concepción), 2012
Erroneous identifi cation of the mussel, Mytilus galloprovincialis (Lamarck 1819) as the specie, Mytilus chilensis (Hupe 1854) in the Bay of Concepcion, Chile Identifi cación errónea del mejillón, Mytilus galloprovincialis (Lamarck 1819) como la especie, Mytilus chilensis (Hupe 1854) en la Bahía de Concepción, Chile