Changes in Morphology and Presence of Pinopodes in Endometrial Cells during the Luteal Phase in Women with Infertility Problems: A Pilot Study (original) (raw)
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Reproductive sciences (Thousand Oaks, Calif.), 2017
Endometriosis is frequently associated with infertility and it is believed that the impairment of endometrial receptivity may be one of the mechanisms involved in this condition. Pinopodes are endometrial cycle-dependent structures that seem to participate in embryo implantation, and alterations in their presence and/or morphology during the window of implantation could affect the endometrial receptivity and be involved in the disease-related infertility. However, the data on pinopodes in these women are scarce and inconclusive. This pilot study aimed to evaluate the cell pattern, including the presence and developmental stage of pinopodes, in eutopic endometrium of infertile patients with and without endometriosis during the window of implantation, using scanning electron microscopy (SEM). Endometrial biopsies were performed using a Pipelle catheter, and 12 samples classified in the window of implantation (6 infertile women with endometriosis and 6 infertile controls) were analyzed...
Fertility and Sterility, 2003
Objective: To assess the temporal and morphologic characteristics of pinopod expression on the surface of endometrium across the secretory phase, in LH-timed endometrial samples in normal, healthy women. Design: Prospective, randomized study. Setting: Academic teaching hospital. Patient(s): Sixty-eight healthy volunteers with proven fertility. Intervention(s): Urinary LH-timed endometrial and blood sampling was performed on each subject on a randomly selected day of the secretory phase. Main Outcome Measure(s): Histologic dating, assessment of pinopods using scanning electron microscopy, and comparison with serum P levels. Result(s): Eighty-six endometrial tissue samples obtained from 68 subjects were evaluated under scanning electron microscopy. Pinopods were first observed on luteal day 5, corresponding with the onset of the midluteal phase increase in serum P levels. Pinopods persisted for the entire duration of the secretory phase, but their morphology changed as the cycle advanced. Conclusion(s): The present findings demonstrate that pinopods are a characteristic feature of the mid to late secretory phase endometrial epithelium, exhibit cycle-dependent changes in morphology, and are most prominent during the putative implantation interval.
Human Reproduction, 1996
In the light and electron microscopical study reported here, we document the structure of the nucleus, nucleolus and nuclear channel system (NCS) in the uterine glandular epithelium in both fertile and infertile women during the early luteal phase. Nuclear volume was found to be larger in the infertile group at day 5 after the luteinizing hormone surge (LH + 5) compared to daymatched fertile subjects. A two-way analysis of variance performed on nucleolar volume data from fertile and infertile women biopsied on days LH + 4, +5 and +6 revealed a significant effect of condition but no effect of day or interaction. Nucleolar volume decreased from day LH + 2 to day LH + 6 in the fertile group, the sharpest decrease occurring between day LH + 3 and day LH + 4. The largest mean volume of the NCS was found at day LH + 5 in the fertile group and day LH + 6 in the infertile group. The results suggest a delay in the development of this organelle in the infertile women. The present study has documented alterations in the nuclei of uterine glandular cells from infertile patients. In these infertile women, there is also a delayed elaboration of the secretory apparatus and this delay correlates well with the delayed/reduced expression of a luteal-specific glycoprotein.
The presence of pinopodes in the human endometrium does not delineate the implantation window
Fertility and Sterility, 2007
Objective: To assess pinopode formation in human endometrium during the luteal phase of the menstrual cycle and in the first trimester of pregnancy. Design: Prospective clinical study. Setting: Outpatient infertility clinics and outpatient family planning clinic. Patient(s): Thirty-two regularly cycling infertile women, 15 regularly cycling fertile women, 9 women receiving elective termination of pregnancy, and 1 woman receiving GnRH agonist and hormone therapy addback. Intervention(s): Endometrial tissue was collected by suction pipelle and examined by scanning electron microscopy for pinopode formation. Main Outcome Measure(s): Endometrial tissue was scored (0, 1, 2, 3, or 4) depending on the percentage of the surface covered in pinopodes (from 0% to Ͼ20% of 100 fields).
Histopathological study of endometrium in cases of infertility in tertiary care hospital
International Journal of Clinical and Diagnostic Pathology, 2019
A study of 513 cases of primary and secondary infertility with 211 control cases was carried out in the department of pathology GMERS Medical College, Junagadh, Gujarat, India. Endometrial specimens were evaluated in the light of menstrual history to find out the incidence of various endometrial changes in cases of infertility. All cases underwent endometrial biopsy ware premenstrual. Haematoxylin and eosin staining was done for dating of endometrium and diagnosis of corpus luteal defect and anovalutory cycles. Periodic Acid Schiff (PAS) was also done. Menstrual problems were seen in 41% of patients. Anovulatory infertility was present in 29% cases. Luteal phase defect was seen in 19% cases. Cystoglandular hyperplasia was seen in 5% cases & tuberculous endometritis was present in 2% cases. Glycogen deficiency was seen in 25.34% cases of luteal phase defect.
BJOG: An International Journal of Obstetrics and Gynaecology, 1990
This study compared endometrial development in the lutcal phase of women with unexplained infertility ( n = 30) with that in wornen with normal fertility (n = 70) by the use of quantitative histological techniques (morphometric analysis) on endometrial specimens which were precisely timed from the luteinizing hormone surge. When overall endometrial development (histological dating) was considered, the proportion of women with unexplained infertility who had retarded endometrial development (20%) was found to be significantly higher than for women with normal fertility (3%) (P<1).01). When individual histological features were considered, women with unexplained infertility were found to have significant deviation from the normal range established fromfertile women in only five of the 14 histological features measured. All of these five features related to the glandular but not to the stromal component of the endometrium; four oi these five features are related to glandular secretory activity.
2013
Abstract. To evaluateendometrial tissues of fertile women and the women with recurrent implantationfailure (RIF) analyzed by using transmissionelectron microscopy (TEM) and MUC1 immunohistochemistry. In this study, 23 patients with RIF and 11 fertile women participated. Endometrial tissuesamples were examined by light and electron microscopes. Additionaly, MUC1immunoreactivity was evaluated. Electron microscopic examination of theendometrial surface epithelial cells of the recurrent implantation failurerevealed lots of cytoplasmic extensions containing secretory vacuoles andirregular microvilli. Furthermore, most of the endometrial cells exhibitedsecretory vacuoles in the supranuclear region. Immunoreactivity of MUC1 wasdetected weakly in the luminal epithelium of the control group whereas thestaining intensity was conspicuous in RIFgroup. Significant differences between the groups with respect to glandularepithelium were found. Endometrial tissues of both fertile women and women w...
A histomorphological pattern analysis in endometrial biopsies of infertile woman
IP innovative publication pvt. ltd, 2019
Introduction: Infertility is an agonising problem to many couples, giving them a sense of hopelessness. The hormonal alterations gives rise to various histological patterns in endometrium. Aims and Objective: To know the various morphological patterns of endometrium in females presenting with infertility (primary/secondary) Materials and Methods: A cross-sectional study in which 237 cases of endometrial biopsies were reviewed along with clinical data, in infertile woman (primary/secondary) from January 2016 to December 2018. The results were analysed and interpreted. Results: Of the 237 biopsies, 196 cases (82.7%) were of primary infertility and 41 cases (17.3%) of secondary infertility. Overall mean age was 28.3 years. The most common endometrial pattern was secretory phase (35.86%) followed by proliferative phase (30.8%). Disordered proliferative phase was seen in 17.7% cases. There were 23 cases of simple cystic hyperplasia and one case of complex hyperplasia. 5 cases each of polyp and tuberculous endometritis were diagnosed and two coincidental cases of endometrial carcinoma. One case was reported as Arias-Stella reaction. Conclusion: Secretory phase of endometrium was the most common morphological pattern which suggests that despite of ovulation there could be other causes that might have led to this ailment. This study also proposes that endometrial biopsies can be used as a primary diagnostic tool especially in cases of unexplained infertility that could be due to tuberculosis or polyps.
Histopathological Study of Endometrium in Infertility: Experience in A Tertiary Level Hospital
BIRDEM Medical Journal, 2018
Background: Infertility refers to inability to achieve conception even after one year of unprotected coitus by a couple. It is a global health problem and affects 8-10% couple worldwide. Infertility can be primary or secondary and there are many causes of infertility involving both male and female partner. A wide range of investigations can be done to find out the causes of infertility. Endometrial biopsy or curettage or aspiration followed by histopathological study is a safe procedure. It not only shows the hormonal response of endometrium but also diagnose other endometrial pathology causing infertility. The study was performed to find out the morphological pattern of endometrium in infertile women in a tertiary care hospital to find out the causes of infertility and subsequent treatment of the patients.Methods: It was a cross sectional prospective study, conducted in the Department of Histopathology and Cytopathology in a tertiary care hospital in Dhaka for a period of two years...
Endometrial pinopodes indicate a shift in the window of receptivity in IVF cycles
Human Reproduction, 1999
The formation of endometrial pinopodes detected by scanning electron microscopy may be a specific marker for uterine receptivity. Aiming to assess the effects of ovarian stimulation on pinopode formation, we examined sequential endometrial biopsies from 17 oocyte donors. Seven normally menstruating women served as controls. Up to four samples were taken from each woman at 24-72 h intervals between days 14 and 24, giving a total of 69 samples. The day of oocyte retrieval was designated day 14 in ovarian stimulation cycles and the day of luteinizing hormone surge was designated day 13 in natural cycles. Endometrial morphology and pinopode numbers were similar in both groups. Fully developed pinopodes appeared in only one sample per cycle, indicating their short life span. However, the cycle day these structures appeared varied up to 5 days between women and the distribution was as follows: day 18 (n⍧2), day 19 (n⍧7), day 20 (n⍧4), day 21 (n⍧3), day 22 (n⍧1) in ovarian stimulation cycles, and day 20 (n⍧2), day 21 (n⍧2), day 22 (n⍧3) in natural cycles. Furthermore, accelerated pinopode formation in ovarian stimulation cycles was positively correlated with day 13 progesterone. Our findings show that ovarian stimulation does not affect endometrial pinopode formation in terms of quantity and life span. The cycle days when pinopodes form are specific to the individual, being on average 1-2 days earlier in ovarian stimulation than in natural cycles. These changes in pinopode expression may reflect shifts in the window of receptivity, resulting in ovo-endometrial asynchrony and limiting implantation success in in-vitro fertilization.