Immunomodulatory effects of Doxycycline in cystic fibrosis airway epithelial cells (original) (raw)

Abstract

Purpose: Cystic Fibrosis is characterized by an intrinsic hyper-immune state. This primary inflammatory condition prior to bacterial infection is supported by clinical observations in CF neonates and young children. Mounting evidence also suggests an exaggerated and prolonged inflammatory response to foreign invaders of the airways. The aim of this work was to evaluate the anti-inflammatory activity of doxycycline on the CF phenotypic bronchial airway epithelium. Methods: The CF compound heterozygote IB3-1 and rescued isogenic S9 cell lines were used to characterize doxycycline's effects on lung parenchyma inflammation and remodeling. Biological markers IL-8 and MMP-9 were analyzed by ELISA and qRT-PCR following stimulation with IL-1β, TNF-α, or Pa-LPS at varying time points. Cytotoxicity was quantified by LDH assays. Results: Diminution of IL-8 release upon doxycycline treatment in a dose dependent manner was observed in both IB3 and S9 cell lines after immune activation with I...

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