BIOREMEDIATION OF EFFLUENT FROM LOCAL TEXTILE INDUSTRY USING Bacillus licheniformis (original) (raw)
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Microbial Treatment of Complex Dyes Used in Local Textile Industries in Nigeria
This study investigated the ability of microorganisms in treating complex dyes used in local textile industries also referred to as cottage industries. Microorganisms used were isolated from soil contaminated with dye effluent from these industries. The isolates were tested for their decolorization ability on single dyes and combination of dyes by preparing simulated dye bath. Gas Chromatography-Mass Spectroscopy (GC-MS) analysis and Brine shrimps cytotoxicity test were carried out using cell free supernatant of the combination of dyes to determine the degradative ability and the detoxification potential of the isolates. Nine bacteria and eight fungi were isolated from the contaminated soil sample. The decolorization ability of the isolates on the singles dyes ranged from 90.11% in Aspergillus fumigatus to 94.95% in Acinetobacter sp. The isolates were able to decolorize combination of dyes having a range of 12.11%-68.01% with Aspergillus ustus having the highest decolorization ability. The untreated combination of dyes had a total of sixteen compounds which were totally broken down into straight chain hydrocarbons. Brine shrimps cytotoxicity test revealed a decrease in the toxicity of the treated combination of dyes. These findings revealed the ability of microorganisms in decolorizing, degrading and detoxifying complex dyes used in textile industries.
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During the last few years it has been demonstrated that several bacterial strains decolorize the synthetic textile dyes. In the present study, the decolorization potential of two textile dyes (malachite green and indigo carmine) by the Bacillus sp. isolated from dye contaminated soil of the local dying houses in Nagpur-India was determined. Decolorization rate was monitored by spectrophotometry. Different parameters such as pH, time and temperature were optimized for the present study. It was found that Bacillus sp. decolorized 95.12% of malachite green (100mg/L) at pH 9.5 and 66.6% of indigo carmine (100mg/L) at pH 6 at 370C within 96 hours under shaking condition. According to the ability of Bacillus sp. to decolorize the textile dyes, this bacterial strain could be used to decolorize and degrade different dyes of textile industry.
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A country like Bangladesh where textile industries are the main source of developing economy, pollution problem from such industries creates a huge risk for the environment. Textile industries discharge a huge amount of effluent containing various harmful chemicals including synthetic dyes that are very stable and threat to the living organisms. This study deals with the potential decolorization and biodegradation of Bemacron Yellow HP-2R (BY), Bemacron Red RS (BR) and Bemacron blue RS 01 (BB) dyes using bacteria isolated from textile effluent. The effluent and soil samples were collected from different locations of discharge point. Only two isolates were screened out after primary screening using dye supplemented nutrient agar media. Following colony morphology, physiology and biochemical analysis, they were presumptively identified as Bacillus sp. and Staphylococcus aureus. They were subjected to decolorization of 0.002 g/l BY, BR and BB dyes. Bacillus sp. showed superior decolori...
Decolourisation of reactive textile dye effluent by using Bacterial strain
The study was investigated the decolorization potentials of certan selected microbial species (bacterial) to an actual textile dyeing industry effluent. All the microbes tested namely (bacterial) Bacillus sp, pseudomonas sp, Acineto bacter, Legionella and Staphylococcus (bacterial) showed greater potential in decolorizing the dye effluent. Bacterial decolorization of textile effluents were critically analysed and discussed. Bioremediation is an option that offers the possibility to destroy harmless various pollutants using natural biological activity.
International Journal of Advanced Scientific Research and Management
Pseudomonas aeruginosa and Brevibacillus choshinensis were enriched and isolated from effluent sample of a textile processing unit using Sterile Minimal medium containing 10% Glucose and incubated at 28 ± 2° C for 7 days. The isolates were identified using standard biochemical tests carried out by KEM Hospital, Pune, India. These isolates were checked for decolourisation of textile dyes and biological stain solutions. It was found that both the bacterial isolates were able to degrade the textile dyes upto 250 ppm concentration. Pseudomonas aeruginosa was further studied for decolourisation of biological stain solutions and was found to decolorize upto 100 ppm of the stain solution. Phytotoxicity of the textile dyes was estimated by measuring the relative changes in seed germination of three plants: Triticum spp. (Wheat), Vigna radiate (Moong seeds) and Brassica juncea (Mustards seeds). Radicle and Plumule growth (length) were recorded after 6 days of exposure to different concentrations of dyes. Toxicity of the degraded dye was also checked which indicated that after decolourization the toxicity of the dye reduced. This property of these bacterial isolates shows a potential that can be utilized for the bioremediation of various textile industrial effluents thus saving the ecosystem from harmful effects of various dyes.
Decolorization And Physico Chemical Analysis Of Textile AZO DYE By Bacillus
International Journal on Applied Bio-Engineering, 2011
Water pollution control is at present one of the major areas of scientific activity. The textile wastewaters are characterized by extreme fluctuations in many parameters such as chemical oxygen demand (COD), biochemical oxygen demand (BOD), pH, colour and salinity. Biotreatment offers a cheaper and environmentally friendlier alternative for colour removal in textile effluents. The ubiquitous nature of bacteria makes them invaluable tools in effluent biotreatment. Potential decolourization and COD removal of the simulated effluent by each isolate were investigated. The gram staining test showed the isolate to be non-motile, gram positive, spore forming, rod-shaped bacteria. Biochemical characterization of the isolate revealed it to be negative for Indole, Methyl Red test. The potential strain was phylogenetically identified as Bacillus. Only effluent-adapted isolates of Bacillus species have relatively high COD removal activities while all the non-adapted isolates have high COD removal capabilities. This study discovered effluent adapted strains of Bacillus with potentials for colour removal and strains of Bacillus with potential use for COD removal. The results suggest that the non-adapted adapted isolates. Reports however indicate that though several microorganisms may seem to have a potential for dye degradation, very few strains can withstand the conditions of dyeing effluents thus the effluent-adapted strains may be better candidates for potential bioremediative uses.
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Textile industries are responsible for one of the major environmental pollution problems in the world, because they release undesirable dye effluents. Textile wastewater contains dyes mixed with various contaminants at a variety of ranges. Therefore, environmental legislation commonly obligates textile factories to treat these effluents before discharge into the receiving watercourses. The present study was an attempt to examine the Decolorization ability of Prominent isolates obtained from the Industrial Effluent Sample (Kocuria rhizophila and Bacillus cereus) for two different commercial Textile Grade Dyes that are ‘Perssian blue’ and ‘Reactive orange 13’. The primary parametric study was done and results were recorded. The isolates showed significant decolorization of both the dyes was identified by bacterial morphology, biochemical tests and also with of Automated Microbial Identification System (VITEK 2) instrument. Various physico-chemical parameters with respect to incubation...
Journal of Applied Sciences and Environmental Management, 2011
The evaluation of some microbial species for the decolourization and degradation of textile dye has been investigated. Six microbial strains were isolated from soil contaminated with textile waste effluents using the spread plate technique and the isolates were identified as bacterial isolates (Pseudomonas fluorescence, Pseudomonas nigificans, and Pseudomonas gellucidium) and fungal isolates ( Aspergillus niger, Proteus morganii and Fusarium compacticum} based on gram staining, morphological and biochemical tests. They were evaluated for their capability to remove colour and degrade dye, reduce chemical oxygen demand (COD) and biological oxygen demand (BOD) levels of textile waste effluents. The results revealed that all the bacterial and fungal isolates have a good potential to remove colour and degrade dye, reduce the COD and BOD levels of the textile waste effluents with percent colour removal, COD and BOD reductions between 39 and 48%, 74 and 97% and 77 and 95%, respectively. Binary mixed culture of Pseudomonas fluorescence and Aspergillus niger was efficiently utilized for the removal of different initial concentration (10, 15, 20, 25 and 30 mg/l) of dye from textile waste effluents. It had a higher percent decolourization than individual isolates. The stirred tank bioreactor was found to be very effective for efficient biotreatment of textile waste effluents. @JASEM
Biodecolourization of Textile Dyes by Effluent Adapted Bacteria
The discharge of textile azo dyes to the environment is an issue of health concern and can harm especially the aquatic ecosystem. The use of microorganisms has been reported to be effective approach for remediation. Five bacterial isolates with the capability of decolourizing textile dyes were isolated from textile effluent and identified as Bacillus thuringiensis (Isolate A2, B6), Bacillus badius (Isolate B5, B9), Bacillus aneurinolyticus (Isolate C2) by different morphological, physiological and biochemical tests. Physicochemical parameters such as temperature, pH and inoculum concentration were optimized for the decolourization process. The optimum temperature, pH and inoculum size for the decolourization of three experimental dyes (Novacron Orange FN-R, Novacron Red FN-R, Terasil Green) were found 30 to 35 °C, 7.0 to 8.0, and 10% (v/v) respectively. The selected bacterial isolates showed different decolourization activities for three experimental dyes. The isolate B5 (Bacillus badius) decolourized 98% of initially added Terasil Green after 48 h of incubation at 35 °C in neutral pH. The present study suggests that the isolated Bacillus sp. can be utilized to treat reactive dyes containing waste water.