Association between oncogenic human papillomavirus type 16 and Killian polyp (original) (raw)
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Ent updates, 2018
Özet: Normal ve tümöral orofaringeal dokuda in situ hibridizasyon ve p16 ekspresyonu ile human papilloma virüsü varl›¤›n›n de¤erlendirilmesi ve klinikopatolojik önemi Amaç: Human papilloma virüs (HPV) pozitif orofaringeal hücreli kanser olgular›nda son y›llarda görülen art›fl, bu virüsün tespitinin klinik önemini art›rmaktad›r. Bu çal›flmada amac›m›z orofaringeal kanser hastalar›m›z›n HPV pozitiflik oranlar›n› bulmak, farkl› HPV tespit yöntemleri olan p16 immünohistokimya (IHC) ve in situ hibridizasyonunun (ISH) etkinli¤ini karfl›laflt›rarak boyanma paternlerini göstermektir. Yöntem: Retrospektif dosya taramas› ile bulunan 23 hasta ve 10 kontrol çal›flmaya dahil edilerek hastalar›n patoloji arflivinden bulunan parafin bloklar›nda p16 IHC ve HPV ISH çal›fl›ld›. Bulgular: Yirmi üç olgunun 7'si p16 pozitif idi. Bunlar›n alt›s› yüksek p16 ekspresyonu gösterirken biri düflük p16 ekspresyonu göstermekteydi. 23 olgunun alt›s› ISH pozitif idi. Yüksek p16 ekspresyonu gösteren tüm olgular HPV ISH pozitif iken düflük ekspresyon gösteren bir olgu HPV ISH negatif idi. Tüm p16 pozitif olgular diffüz p16 ekspresyonu göstermekteydi, dolay›s›yla tümör heterojenitesi göstermemekteydi. Sonuç: Yüksek p16 ekspresyonu (>%70) HPV pozitifli¤inin güvenilir bir göstergesidir ve p16 IHC ile kombine etmek spesifitesini art›rmak-tad›r. Olgular tümör heterojenitesi göstermemekte, dolay›s›yla al›nan az miktarda biyopsi parças›nda bile p16 ekspresyonunun gözlenmesi bize tüm tümöral dokuda p16 ekspresyonu oldu¤unu göstermektedir.
1994
Cancer-free tissues from various anatomical subsites in the head and neck were examined by the polymerase chain reaction (PCR) for the incidence of human papillomavirus (HPV) types 16 and 18. We detected HPV-16 DNA in 9 of 103 samples (8.7%), including specimens from the paranasal sinuses, tonsil, hypopharynx and larynx. However, no HPV-16/18 DNA was detected by Southern hybridization in these 9 samples. The significance of the presence of HPV-16 DNA in non-cancer tissues is still unknown, but PCR detection only of high-risk HPV DNA in head and neck cancer should be evaluated cautiously because of its ubiquity in this region.
Journal of Craniofacial Surgery, 2014
Carcinogenesis concerns several changes that eventually result in the inactivation of tumor suppressor genes and activation of protooncogenes, leading to loss of cell cycle control. Inactivation of p16 seems to be an early event in this process and occurs in approximately 80% of squamous cell carcinoma cases. The aims of this study were to evaluate the immunohistochemical expression of p16 protein in oral and oropharyngeal squamous cell carcinoma cases, with both the tumoral area itself and its surgical margin being analyzed (dysplastic areas and histologically normal epithelium adjacent to carcinoma), and to verify the presence of human papillomavirus (HPV) and its relation to p16 expression. Paraffin-embedded biopsy tissues from 26 patients, 13 with oral squamous cell carcinoma and 13 with oropharyngeal squamous cell carcinoma, comprised the analyzed samples. To detect HPV, a nested polymerase chain reaction test using PGMY 09/11 and GP5 + /GP6 + primers and visualization of the product on a 2% agarose gel was performed. Demographic data were obtained from medical records. The results showed low expression of p16 in the tumor area (38.46%), compared with surgical margins in the histologically normal epithelium (84.6%) and dysplastic areas (57.7%). These findings indicate the inactivation of p16 in the process of malignant transformation. The association described in the literature between expression of p16 and presence of HPV could not be verified in this study, because none of the cases was HPV positive.
High-risk human papillomavirus infection and p16INK4a protein expression in laryngeal lesions
Pathology - Research and Practice, 2008
A total of 88 samples of laryngeal lesions (23 vocal cord nodules (VCNs), 23 papillomas (PAs), 18 dysplasias (DYs), and 24 carcinomas (CAs)) were analyzed for p16 INK4a protein (p16) expression by immunohistochemistry and for high-risk human papillomavirus (HR-HPV) infection using chromogene in situ hybridization (CISH) and polymerase chain reaction (PCR).
The characteristics of human papillomavirus DNA in head and neck cancers and papillomas
Journal of Clinical Pathology, 2005
To determine the prevalence, type, physical state, and viral load of human papillomavirus (HPV) DNA in cases of head and neck cancer and recurrent respiratory papillomatosis (RRP). Methods: The prevalence and type of HPV DNA was determined in 27 fresh frozen tissue specimens from patients with head and neck cancers and 16 specimens from 10 patients with RRP by MY09/MY11 and GP5+/ GP6+ nested polymerase chain reaction (PCR) and subsequent restriction enzyme cleavage. The physical state of HPV DNA was analysed by E1, E2, and E1E2 specific PCRs and Southern blot hybridisation (SBH). Results: HPV DNA was detected in 13 of 27 cancers and 10 of 10 papillomas. Both low risk HPV-6 and HPV-11 and high risk HPV-16 were present in cancers in low copy numbers, whereas papillomas exclusively harboured low risk HPV-6 and HPV-11. E1E2 PCRs failed to determine the physical state of HPV in cancers except one case where HPV-6 DNA was integrated. In contrast to cancers, all papillomas showed the episomal state of HPV DNA and a relatively higher viral load. Conclusions: Based on the prevalence, type, physical state, and copy number of HPV DNA, cancers and papillomas tend to show a different HPV DNA profile. The 100% positivity rate of low risk HPV types confirms the role of HPV-6 and HPV-11 in the aetiology of RRP.
P16(INK 4a) and Ki-67 expression in human papilloma virus-related head and neck mucosal lesions
Investigación clínica, 2015
Human papilloma virus (HPV) is postulated as a risk factor in the etiology of some specific mucosal pathologies in the head and neck regions. Despite the frequent use of p16(INK4a) as a surrogate marker for HPV-infection, there is still controversy with respect to its reliability. This study has been undertaken to assess the potential role of p16(INK 4a) and Ki-67 expression in HPV-related lesions. The study was conducted on 71 specimens of oral, tonsillar and laryngeal lesions which comprised 25 dysplasia and 46 papilloma specimens. Specimens were immunohistochemically stained for p16(INK4A) and Ki-67 proteins. HPV DNA was determined by one step multiplex polymerase chain reaction. HPV DNA was detected in 33.8% of all lesions. Tonsil and larynx lesions showed significant differences with oral lesions for HPV positivity (p < 0.001). p16(INK 4a) over-expression was seen in 56.5% of papilloma and 60% of dysplasia specimens. HPV status showed a positive correlation with p16(INK 4a) ...
Journal of Histochemistry & Cytochemistry, 2003
S U M M A R Y Human papillomavirus (HPV) is believed to promote the oncogenic process, and the correlation between viral oncoproteins and dysfunction of p16 INK4A tumor suppressor protein in oral lesions is controversial. To test the hypothesis that anogenital HPV types participate in disruption of the regulation of p16 INK4A suppressor protein in oral lesions, we analyzed 46 oral biopsy specimens for the presence of HPV 6/11 and 16/18 by in situ hybridization (ISH) and for p16 INK4A expression by immunohistochemistry (IHC). Eighteen (39%) of the 46 oral lesions were HPV-positive and 28 (61%) were HPV-negative. HPV 6/11 DNA was found in 5 (11%) and HPV 16/18 in 13 (28%) of 46 biopsies. Nine of the 18 HPV-positive oral lesions (50%), assessed by catalyzed signal amplification coupled to ISH (CSA-ISH), gave high-intensity p16 INK4A immunostaining. Focal and diffuse patterns were observed in 11/13 (77%) lesions with HPV 16/18, focal immunopositivity in 3/5 (80%) with HPV 6/11, and negative or sporadic p16-labeling in 18/28 (64%) without the presence of HPV DNA. These results showed a strong association between overexpression of p16 protein and malignant oral lesions, mainly those infected by HPV 16/18. We can conclude that high-risk HPV types are associated with p16 overexpression, and p16 may serve as a biomarker in oral cancer related to high-risk HPV infection.
INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH, 2020
Aim of this study is to assess the expression of p16 as an immunohistochemical marker (which is a predictor or surrogate marker of high risk HPV infection) in oropharyngeal, head and neck squamous cell carcinoma and its correlation with different histomorphological features. Our study was an observational, descriptive study with cross-sectional design, done in the Department of Pathology, in collaboration with the Department of Plastic Surgery. It was conducted on a group of patients admitted in Department of plastic Surgery, R G KarMedical College & Hospital, and Kolkata. The samples included 54 specimens of, oropharyngeal & head neck SCC. Here, in this study, the expression of P16 in oropharyngeal & head neck SCC was shown& correlated with their histomorphological features. In present study median age of p16 positive patients were 54 years and male were more commonly affected than female. According to Socio economic status p16 positive cases were most commonly found in Lower statu...