Production of Lipid and Fatty Acids during Growth of Aspergillus terreus on Hydrocarbon Substrates (original) (raw)
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Baghdad Science Journal, 2021
In the present study, the growth and total lipid contents of two oleaginous fungal isolates Aspergillus terreus, Aspergillus fumigatus were compared in different nitrogen and organic carbon sources. Artificially the fungi were cultured on media consisting of various mono-or di-or polysaccharides and peptone or yeast extract as elementary sources for carbon and nitrogen, respectively. Media containing sucrose /yeast extract or glucose/ yeast extract were the most effective for lipid production from fungal, during two weeks incubation period, the highest biomass of dry weight was (19.6 , 18.8) g / L , (25.8 , 30.5) g /L and lipid yield (1, 0.97)g/L, (0.65, 0.65) g/ L for two isolates Aspergillus terreus and Aspergillus fumigatus respectively then followed maltose/ yeast extract then starch / yeast extract. Analysis of fatty acid produced by two fungal isolates using Gas Chromatography showed the existence of a wide range of fatty acids, these fatty acids were together saturated and unsaturated. The results revealed that the unevenness in the fatty acids composition mainly depends on the type of carbon and nitrogen sources. The existence of saturated and mono saturated fatty acids in A.fumigatus, A. terreus prove that they are good candidate for biodiesel production.
Grasas y Aceites, 2020
Oleaginous fungi have recently gained increasing attention among different microorganisms due to their ability for lipid production for the preparation of biofuel. In the present study, a locally isolated fungus E45, identified genetically as Aspergillus sp. strain EM2018, was found to produce 25.2% of the total lipids content of its dry cell weight (DCW). Optimization of culture conditions was performed and lipid accumulation increased by about 2.4 fold (from 25.2% to 60.1% of DCW) when the fungus was grown for seven days in the potato dextrose (50 g/L) liquid medium at pH 5.0, incubation temperature at 30 ºC and inoculum size of 2 × 106 spore/mL. Supplementation of the medium with yeast extract and NaNO3 at a concentration of 0.05% as organic and inorganic nitrogen sources, respectively, increased lipid production (53.3% lipid/dry biomass). Gas chromatography analysis of fungal lipids revealed the presence of saturated (mainly palmitic acid C16:0 (33%) and lignoceric acid C24:0 (...
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