Scanning Electron Microscope StudyofSaccharomyces cerevisiae Spheroplast Formation (original) (raw)

This study presents a detailed examination of spheroplast formation in the yeast Saccharomyces cerevisiae NCYC366 using scanning electron microscopy with a resolution of 4 nm. Key morphological features were identified, including warty structures at bud scars, a beady appearance of the cell wall during digestion, and the presence of distinct indentation and furrows on the plasma membrane. Compared to previous studies with transmission electron microscopy, this research enhances understanding of spheroplast topography and membrane stability during wall digestion.