Biarsenical labeling of tetracysteine-tagged proteins for tracking existing and newly synthesized pools of proteins (original) (raw)

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) contains the major glycoprotein, GP5, as well as three other minor glycoproteins, namely, GP2a, GP3, and GP4, on the virion envelope, all of which are required for generation of infectious virions. To study their interactions with each other and with the cellular receptor for PRRSV, we have cloned each of the viral glycoproteins and CD163 receptor in expression vectors and examined their expression and interaction with each other in transfected cells by coimmunoprecipitation (co-IP) assay using monospecific antibodies. Our results show that a strong interaction exists between the GP4 and GP5 proteins, although weak interactions among the other minor envelope glycoproteins and GP5 have been detected. Both GP2a and GP4 proteins were found to interact with all the other GPs, resulting in the formation of multiprotein complex. Our results further show that the GP2a and GP4 proteins also specifically interact with the CD163 molecule. The carboxy-terminal 223 residues of the CD163 molecule are not required for interactions with either the GP2a or the GP4 protein, although these residues are required for conferring susceptibility to PRRSV infection in BHK-21 cells. Overall, we conclude that the GP4 protein is critical for mediating interglycoprotein interactions and, along with GP2a, serves as the viral attachment protein that is responsible for mediating interactions with CD163 for virus entry into susceptible host cell.

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References (57)

  1. GP2a-EcoRI-For .......................................................................ATATATGAATTCGCCACCATGAAATGGGGTCCATGC GP2a-SphI-Rev..........................................................................ATATATGCATGCTCACCGTGAGTTCGAAGG GP2a-NheI-For..........................................................................ATATGCTAGCGCCGCCACCATGAAATGGGGTCCATGC GP2a-FLAG-EcoRI-Rev..........................................................ATATGAATTCTCACTCGAGCTTGTCATCGTCGTCCTTGTAGTCCATCCGTG AGTTCGAAGGAAAAATTGC
  2. GP3-EcoRI-For .........................................................................ATATAGAATTCGCCACCATGGCTAATAGCTGTGC GP3-BamHI-Rev .......................................................................TATATGGATCCCTATCGCCGCGCGGC GP4-EcoRI-For .........................................................................ATATAGAATTCGCCACCATGGCTGCGCCCCTTC GP4-BamHI-Rev .......................................................................TATATGGATCCTCAAATTGCCAGTAAGATG GP5-EcoRI-For .........................................................................GCCGGAATTCGGAGCCGCCGCCACCATGTTGGGGAGATGCTTGAC GP5-HindIII-XhoI-Rev ............................................................ATCACTCGAGAAGCTTCTAAAGACGACCCCATTGTTC CD163-HindIII-For...................................................................ATATAAGCTTATGGACAAACTCAGAATGGTGCTAC CD163-XhoI-Rev ......................................................................ATATCTCGAGTCATTGTACTTCAGAGTGGTCTCCTG a Primer sequences are in the 5Ј to 3Ј direction. Restriction enzyme sites in the primers are underlined. VOL. 84, 2010 PRRSV GP AND CD163 INTERACTIONS 1733 on April 23, 2012 by UNIV OF NEBRASKA-LINCOLN http://
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  57. 1740 DAS ET AL. J. VIROL. on April 23, 2012 by UNIV OF NEBRASKA-LINCOLN http://