Evaluation of dentin formed in autogenous tooth transplantation in the dog: a comparison between one- and two-stage surgical techniques (original) (raw)

2012, Dental Traumatology

Autogenous tooth transplantation could be an alternative way to restore the arch if there is a suitable donor tooth available (1, 2). Successful tooth transplantation depends upon the optimal and uneventful healing of the periodontium (3, 4). It depends upon the vitality of remaining periodontal ligament cells in the donor root, the shape and the site of the recipient socket and the vascularity of the recipient bed (5-8). Immature teeth are more useful for transplantation than fully mature teeth (4). To improve nutrition and preserve cell activity in these tissues, Nethander et al. (6), Katayama et al. (7) and Ferreira et al. (9) suggested that teeth should be transplanted to the sockets with regenerative tissues, which may reduce the root resorption and the circulation and innervations recover the original pulp tissue, and dentin development continues after transplantation of immature teeth. The aim of this study was to examine, by an experimental study in dogs, the disturbance in formation and calcification of dentin in teeth transplanted to a recipient bed, prepared immediately before transplanta-tion (the one-stage method) or in which the tissue was under regeneration during 7 days (the two-stage method). Material and methods Before the start of the experiment, application was made to the Direcc¸a˜o de Servic¸os de Meios de Defesa da Sau´de, Bem-estar e Alimentac¸a˜o Animal da Direcc¸a˜o Geral de Veterina´ria (Portugal). The animal experimental procedures were performed in Estac¸a˜o Zoote´cnica Nacional (EZN)-Instituto de Tecnologia Biome´dica, Santare´m, Portugal, in accordance with the International Guiding Principles for Animal Research. The animals were acquired from Universidade de Co´rdova, Espanha, maintained in the EZN during the experimental period and were observed daily by the doctor and technicians of the EZN. Twelve non-carious and periodontally sound incisors and twelve non-carious and periodontally sound premolars from three male Beagles (age 5 months; body weight