Bioleaching of Manganese Oxides at Different Oxidation States by Filamentous Fungus Aspergillus niger (original) (raw)

Oxalate formation by Aspergillus niger on minerals of manganese ores

American Mineralogist, 2021

Microscopic fungi (micromycetes) play an important role in rock alteration, often leading to the formation of insoluble biogenic oxalates on their surface. Oxalate crystallization under the influence of fungus Aspergillus niger (one of the most active stone destructors) was studied in vitro conditions on following Mn,Ca-bearing minerals of manganese ores: todorokite (Na0.36,Ca0.09,K0.06,Sr0.03, Ba0.02)0.56(Mn5.53,Mg0.47)O12∙3–4H2O and kutnohorite (Ca0.77,Mn0.23)(Mn0.74,Fe0.14,Mg0.11)(CO3)2. The underlying minerals and the products of their alteration were investigated via powder and single-crystal X-ray diffraction, optical microscopy, SEM and EDX methods. It was shown that more intense leaching of Ca-ions (compared to Mn-ions) from todorokite and kutnohorite leads to an earlier crystallization of calcium oxalates (predominantly whewellite) compared to manganese (lindbergite, falottaite). Crystallization of manganese oxalates on the surface of kutnohorite occurs in a more acidic (co...

Oxalate formation by Aspergillus niger on manganese ore minerals

2021

Microscopic fungi (micromycetes) play an important role in rock alteration often leading to formation of insoluble biogenic oxalates on rock/mineral surfaces. Oxalate crystallization under the influence of fungus Aspergillus niger (one of the most active stone destructors) was studied in vitro on two Mn,Ca-bearing minerals of manganese ores: todorokite (Na0.36,Ca0.09,K0.06,Sr0.03,Ba0.02)0.56(Mn5.53,Mg0.47)O12∙3-4H2O and kutnohorite (Ca0.77,Mn0.23)(Mn0.74,Fe0.14,Mg0.11)(CO3)2. The underlying minerals and the products of their alteration were investigated using powder and single crystal X-ray diffraction, optical microscopy, SEM and EDX methods. It was shown that a more intense leaching of Ca-ions (compared to Mn-ions) from todorokite and kutnohorite leads to an earlier crystallization of calcium oxalates (predominantly whewellite) compared to manganese oxalates (lindbergite, falottaite). Crystallization of manganese oxalates on the surface of kutnohorite occurs in more acidic (compar...

Colonization, penetration and transformation of manganese oxide nodules by Aspergillus niger

Environmental Microbiology

In this study, the ability of the geoactive fungus Aspergillus niger to colonize and transform manganese nodules from the Clarion-Clipperton Zone in both solid and liquid media was investigated. Aspergillus niger was able to colonize and penetrate manganese nodules embedded in solid medium and effect extensive transformation of the mineral in both fragmented and powder forms, precipitating manganese and calcium oxalates. Transformation of manganese nodule powder also occurred in a liquid medium in which A. niger was able to remove the fine particles from suspension which were accumulated within the central region of the resulting mycelial pellets and transformed into manganese oxalate dihydrate (lindbergite) and calcium oxalate dihydrate (weddellite). These findings contribute to an understanding of environmental processes involving insoluble manganese oxides, with practical relevance to chemoorganotrophic mineral bioprocessing applications, and, to the best of our knowledge, represent the first demonstration of fundamental direct and indirect interactions between geoactive fungi and manganese nodules.

Colonization, penetration and transformation of manganese oxide nodules by Aspergillus niger

Environmental Microbiology, 2019

In this study, the ability of the geoactive fungus Aspergillus niger to colonize and transform manganese nodules from the Clarion-Clipperton Zone in both solid and liquid media was investigated. Aspergillus niger was able to colonize and penetrate manganese nodules embedded in solid medium and effect extensive transformation of the mineral in both fragmented and powder forms, precipitating manganese and calcium oxalates. Transformation of manganese nodule powder also occurred in a liquid medium in which A. niger was able to remove the fine particles from suspension which were accumulated within the central region of the resulting mycelial pellets and transformed into manganese oxalate dihydrate (lindbergite) and calcium oxalate dihydrate (weddellite). These findings contribute to an understanding of environmental processes involving insoluble manganese oxides, with practical relevance to chemoorganotrophic mineral bioprocessing applications, and, to the best of our knowledge, represent the first demonstration of fundamental direct and indirect interactions between geoactive fungi and manganese nodules.

Bioleaching of manganese by Aspergillus sp. isolated from mining deposits

Chemosphere, 2017

A comprehensive study on fungus assisted bioleaching of manganese (Mn) was carried out to demonstrate Mn solubilization of collected low grade ore from mining deposits of Sanindipur, Odisha, India. A native fungal strain MSF 5 was isolated and identified as Aspergillus sp. by Inter Transcribed Spacer (ITS) sequencing. The identified strain revealed an elevated tolerance ability to Mn under varying optimizing conditions like initial pH (2, 3, 4, 5, 6, 7), carbon sources (dextrose, sucrose, fructose and glucose) and pulp density (2%, 3%, 4%, 5% and 6%). Bioleaching studies carried out under optimized conditions of 2% pulp density of Mn ore at pH 6, temperature 37 °C and carbon dosage (dextrose) resulted with 79% Mn recovery from the ore sample within 20 days. SEM-EDX characterization of the ore sample and leach residue was carried out and the micrographs demonstrated porous and coagulated precipitates scattered across the matrix. The corresponding approach of FTIR analysis regulating ...

A comparative study for Bioleaching of various manganese ores

A native microorganism, Penicillium citrinum was isolated from the top soil of a manganese mine. Based on its efficiency for manganese solubilisation, it was utilized for the leaching of a low-grade manganese ore. The effects of various parameters such as pulp density, particle size, sucrose concentration, inoculum size and bioleaching duration on manganese ore were studied. The optimised conditions for maximum solubilization of the manganese ore (64.58% Mn) were : a particle size of 45µm; a pulp density of 2% (w/v); a sucrose concentration of 10% (w/v); an inoculum dosage of 10% (v/v); and a 30 day duration.

Manganese: Its Speciation, Pollution and Microbial Mitigation

International Journal of Applied Sciences and Biotechnology, 2013

Manganese is known to be one of the essential trace elements and has plenty of applications. Inspite of its essential nature, concerns are arising due to its toxic nature at higher concentration. Several methods of removing manganese from environment have been proposed during the last few decades. However, the most favourable option based on cost-effectiveness, performance, and simplicity is still under investigation. The current review summarizes updated information on various technical aspects on manganese, including chemical nature, speciation, toxicity and remediation strategies. The review starts with covering the major sources of manganese, its interaction with biological biomolecules causing toxicity. This is followed by its speciation in environment, describing both biotic and abiotic processes. The biotic processes describe the role of microorganisms in the oxidation/ reduction of various oxidation states of manganese. Whereas, abiotic processes mainly describes the role of...

The Structure of manganese oxide formed by the fungus Acremonium sp. strain KR21-2

Geochimica et Cosmochimica Acta, 2009

Manganese oxides are observed to form by the oxidation of aqueous solutions of Mn(II) catalyzed by the action of microorganisms. In contrast to the widely studied material produced by bacteria, manganese oxide phases produced by the action of fungi have received only limited attention. A detailed study of the MnO x material produced by the action of the fungus Acremonium KR21-2, utilizing X-ray diffraction, XANES, EXAFS and transmission electron microscopy is reported. The MnO x material is produced as small crystalline particles which adopt a todorokite-like tunnel structure, in striking contrast to previously reported microbial MnO x materials which adopt layered birnessite-type structures. ICPMS measurements reveal there are no templating metal ions present in the fungally mediated MnO x material, in contrast to analogous bacterially mediated material, suggesting these cations play a critical role in determining the structure of the material precipitated. A phylogenetic analysis places KR21-2 with other Acremonium species in the Hypocreales.

Bioleaching of manganese (IV) oxide and application to its recovery from ores

Biotechnology Letters, 1990

Bioleaching of manganese (IV) oxide with Thiobacillus thiooxidans has been studied in media with and without sulfur, ferrous sulfide and ferrous sulfate. The knowledge of the role played by the bacteria and the reducing substances suggest that the leaching of manganese (IV) ores through the use of thiobacteria is only justified when suitable amounts of sulfur or metal sulfides are present.